Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats

Beheshtian, Azadeh; Salmasi, Amirali; Payabvash, Seyedmehdi; Kiumehr, Saman; Ghazinezami, Behtash; Rahimpour, Sina; Tavangar, Seyed Mohammad; Dehpour, Ahmad Reza

doi:10.1007/s00345-008-0243-6

Cited by 96 publications

(79 citation statements)

References 23 publications

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“…They reported increased levels of antioxidant enzyme levels, decreased levels of lipid peroxidation, and germ cell apoptosis. 32 Similarly protective effects of low-dose sildenafil on contralateral testis were reported in another study; 15 however, they found high-dose sildenafil had no protective effect on testis histopathologically or biochemically. In another study, vardenafil administration was found to have protective effects in rat testis after 30 minutes of torsion and four hours reperfusion.…”

mentioning

confidence: 63%

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

Ozmerdiven

Çoşkun

Kaygısız

et al. 2017

CUAJ

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Cite as: Can Urol Assoc J 2017;11(1-2):E19-25. http://dx.doi.org/10.5489/cuaj.3872 Published online January 12, 2017 AbstractIntroduction: Nitric oxide (NO) plays an important role in the ischemia and reperfusion process. In this study, we aimed to examine the effect of L-arginine, tadalafil, and their combination for prevention of the ischemia reperfusion injury after testis torsion in rats. Methods: A total of 40 adult, male Sprague-Dawley rats were allocated into five groups. Three hours of left testicular torsion was performed in each group, excluding the control group. While the ischemia reperfusion (I/R) group had no treatment, I/R + Arg group received L-arginine, I/R + Td group received tadalafil and I/R + Arg + Td group received tadalafil and L-arginine 30 minutes before the detorsion. Then the left testis was untwisted for four hours of reperfusion. After bilateral orchiectomy, lipid peroxidation (LPx) and glutathione (GSH) activities were examined in testicular tissue. Spermatogenesis was evaluated with Johnsen's score. Results: LPx levels of the I/R group were found to be significantly higher than for groups that received drugs for both testes (p<0.001). GSH levels of the combination group were higher than I/R group in ipsilateral testis (p<0.01) and it was significantly higher than other groups for contralateral testis (p<0.001 for I/R group, p<0.01 for I/R + Arg, p<0.05 for I/R + Td). Mean Johnsen's score of the I/R group was found to be significantly lower than treatment groups in ipsilateral testis (p<0.001 for I/R + Arg + Td group, p<0.01 for other treatment goups) and contralateral testis (p<0.001). The mean Johnsen score of the combination group was significantly higher than that of other treatment groups in ipsilateral testis (p<0.05) and it was significantly higher than in the I/R + Td group in the contralateral testis (p<0.05). Conclusions: L-arginine, tadalafil, and combination of these two molecules showed protective effect against ischemia/reperfusion injury for both testes after unilateral testis torsion.

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mentioning

confidence: 63%

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

Ozmerdiven

Çoşkun

Kaygısız

et al. 2017

CUAJ

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“…Similar to MDA, SOD activity reflects the oxidative stress in tissue. 12 Alternative medications that may aid in restoration of reperfusion injury after restoration have been investigated. Nevertheless, no consensus could be established on the indications, effectivity, dosage, and duration of these molecules on alleviation of I/R injury.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, no consensus could be established on the indications, effectivity, dosage, and duration of these molecules on alleviation of I/R injury. 5,11,12 Pyrrolidine dithiocarbamate is a low-molecular weight thiol compound used as an antioxidant against the toxic impacts of free radicals. This beneficial effect of PDTC is linked with its thiol group, which acts by neutralizing reactive oxygen species.…”

Section: Discussionmentioning

confidence: 99%

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Kemahlı

Yildiz

Fırat

et al. 2016

CUAJ

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Introduction: The aim of this experimental study was to investigate the histopathological and biochemical effects of pyrrolidine dithiocarbamate, an antioxidant and inhibitor of NF-kβ, on ischemiareperfusion injury in rats. Methods: A total of 21 male Wistar-Albino rats were randomly distributed into three groups as sham group (Group 1), ischemia-reperfusion (I/R) group (Group 2) and I/R with pyrrolidine dithiocarbamate (PDTC) group (Group 3). Left testicles of rats in Groups 2 and 3 underwent testicular torsion of 720° for four hours and 100 mg/ kg of PDTC was administered intraperitoneally prior to detorsion in Group 3. An hour after detorsion process, left orchiectomies were performed and 5 ml of intracardiac blood samples were drawn from rats in all three groups. Histopathological examination of testis tissues performed and measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in blood samples were taken. Results: Elevated levels of MDA and decreased SOD activity, together with decreased Johnson tubular biopsy scores consistent with I/R injury were observed in Group 2 (p<0.05). Group 1 and Group 3 were similar in terms of MDA levels, SOD activity, and Johnson scores (p>0.05). Conclusions: Our results indicated that PDTC may have beneficial effects for alleviation of I/R injury in testicular tissue in rats. Understanding the underlying mechanisms and exploration of its diagnostic and therapeutic potential requires further randomized, controlled trials on a larger scale.

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“…2,16,17 Deneysel modellerde oksijen radikal süpürücüleri gibi kimyasal ajanlar, İ/R hasarını engellemede başarılı bulunmuş, fakat ciddi yan etkileri nedeniyle bunların çok azı bu amaçla klinik kullanıma girebilmiştir. 18,19 Deksmedetomidinin testiküler İ/R üzerine olan etkilerinin araştırıldığı bu çalışmada, kolay temin edilmesi, deneysel İ/R modellerinin uygulandığı ve deksmedetomidinin antiinflamatuar etkinliğinin incelendiği çalışmalarda kullanılmış olması nedeniyle deney hayvanı olarak sıçan seçil-miştir. 2,[8][9][10] Deneysel çalışmalarda farklı torsiyon derecelerinin araştırıldığı dikkati çekmiştir.…”

Section: Discussionunclassified

The Effects of Two Different Doses of Dexmedetomidine in a Testicular Ischemia-Reperfusion Model in Rats

Bektaş¹,

Özzeybek²,

Günenç³

et al. 2012

Turkiye Klinikleri J Med Sci

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766 Sıçanlarda Testiküler İskemi ReperfüzyonModelinde İki Farklı Dozda Deksmedetomidinin Etkileri Ö ÖZ ZE ET T A Am ma aç ç: : Yapılan çalışmalarda, deksmedetomidinin antiinflamatuar etkinlik gösterdiği belirlenmiştir. Bu araştırmanın amacı, sıçanda testiküler iskemi/reperfüzyon modelinde, farklı iki dozda uygulanan deksmedetomidinin etkilerini araştırmaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Ağırlıkları 300-350 g arasında değişen 28 adet erkek Albino Wistar sıçan dört gruba ayrılmıştır. Tüm gruplardaki sıçan-larda sol testis ve spermatik kordon sol inguinal insizyonla serbestleştirildi. Grup 1'deki (Kontrol) (n=7) sıçanların sol testisleri, herhangi bir işlem yapılmadan skrotuma yerleştirildi. Diğer gruplara sol testise 1 saat torsiyon, 4 saat detorsiyon uygulandı. Grup 2'ye (İskemi/reperfüzyon) (İ/R, n=7) ilaç uygulaması yapılmadı. Torsiyon işleminin başlangıcının 30. dakikasında Grup 3 (İ/R+50D) (n=7) sıçanlara 50 µg/kg, Grup 4 (İ/R+100D) (n=7) sıçanlara ise 100 µg/kg deksmedetomidin intraperitoneal yolla enjekte edildi. Deney sonunda testis dokusundaki reperfüzyon hasarının derecesi doku malondialdehit (MDA) ve nitrik oksit (NO) düzeyleri ölçümü ile germ hücre hasarı ise alınan testiste histopatolojik olarak Cosentino Skalası değerlendirilerek yapıldı. B Bu ul lg gu ul la ar r: : İ/R grubunda MDA düzeyleri, Kontrol, İ/R+50D ve İ/R+100D gruplarına kıyasla belirgin şekilde daha yüksek bulundu (p<0,001). İ/R+50D ve İ/R+100D grupları arasındaki fark da istatistiksel olarak anlamlı bulundu (p=0,01). NO düzeyleri de İ/R grubunda, Kontrol, İ/R+50D ve İ/R+100D gruplarına kıyasla daha yüksek bulundu (p=0,003). Histopatolojik skorlar ise İ/R grubunda, İ/R+50D ve I/R+100D gruplarındakinden daha yüksek tespit edildi (p=0,001). S So on nu uç ç: : Sıçanda testiküler iskemi reperfüzyon modelinde 50 ve 100 µg/kg'lık dozlarda uygulanan deksmedetomidinin reperfüzyon hasarını doza bağımlı olarak azalttığı kanısına varıldı.A An na ah ht ta ar r K Ke el li im me el le er r: : Deksmedetomidin; reperfüzyon hasarı; spermatik kordonun dönmesi A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Dexmedetomidine is known to have anti-inflammatory activity in both ischemic and toxic inflammatory response models. In this study, the effects of two different doses of dexmedetomidine were investigated in a rat model of testicular ischemia-reperfusion. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Twenty eight rats weighing 300-350 g were divided into four groups. The left testis and spermatical cord were liberated by left inguinal incision in all groups. Sham group (Group 1) (n=7) did not receive any other procedure. One hour of torsion and four hours of detorsion were applied to the other groups. The rats in ischemia/reperfusion group (Group 2) (I/R, n=7), did not receive any drug. Dexmedetomidine 50 µg/kg for Group 3 (I/R+50D, n=7) and 100 µg/kg for Group 4 (I/R+100D, n=7) was injected intraperitoneally after 30 mins of testicular torsion. To grade the testicular reperfusion injury tissue malondialdeh...

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Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats

Abstract: Sildenafil administration during testicular torsion decreased ischemia/reperfusion cellular damage. The results of biochemical studies suggest that, reduction of oxidative stress by sildenafil may have a major role in its cytoprotective effects.

Cited by 96 publications

References 23 publications

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

The protective effect of L-arginine, tadalafil, and their combination in rat testes after ischemia and reperfusion injury

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

The Effects of Two Different Doses of Dexmedetomidine in a Testicular Ischemia-Reperfusion Model in Rats

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