Protective effects of SIRT6 against lipopolysaccharide (LPS) are mediated by deacetylation of Ku70

Zhang, Lin; Bai, Li; Ren, Qihui; Sun, Guohui; Si, Yajing

doi:10.1016/j.molimm.2018.07.009

Cited by 15 publications

(10 citation statements)

References 20 publications

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“…SIRT6 overexpression induces massive apoptosis in cancer cells but not in normal cells. SIRT6 can drive apoptosis by deacetylating key cell signaling molecules, such as KU70, Bax, and P53, in response to DNA damage and oxidative stress [70,111,112].…”

Section: Sirtuins and Apoptosis In Cancersmentioning

confidence: 99%

The Roles of Sirtuin Family Proteins in Cancer Progression

Zhao

Hou

et al. 2019

Cancers

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Sirtuin family members are characterized by either mono-ADP-ribosyltransferase or deacylase activity and are linked to various cancer-related biological pathways as regulators of transcriptional progression. Sirtuins play fundamental roles in carcinogenesis and maintenance of the malignant phenotype, mainly participating in cancer cell viability, apoptosis, metastasis, and tumorigenesis. Although sirtuin family members have a high degree of homology, they may play different roles in various kinds of cancer. This review highlights their fundamental roles in tumorigenesis and cancer development and provides a critical discussion of their dual roles in cancer, namely, as tumor promoters or tumor suppressors.

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Section: Sirtuins and Apoptosis In Cancersmentioning

confidence: 99%

The Roles of Sirtuin Family Proteins in Cancer Progression

Zhao

Hou

et al. 2019

Cancers

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“…As an NAD+-dependent lysine deacetylase, sirtuin 1 (SIRT1) plays a key role in metabolism, inflammation, and aging [5]. At the same time, SIRT6 is associated with the treatment of LPS-induced pulpitis [6]. The expression of SIRT6 in human DPSCs was downregulated using LPS treatment.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-506 Is Involved in Regulation of the Occurrence of Lipopolysaccharides (LPS)-Induced Pulpitis by Sirtuin 1 (SIRT1)

Wang

Deng

et al. 2019

Med Sci Monit

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Departmental sources Background: Toothache often occurs with pulpitis. Lipopolysaccharide (LPS) is produced by gram-negative bacteria, and its accumulation is related to clinical symptoms of pain. MicroRNAs (miRNAs) display anti-inflammatory potential due to their direct regulation of cellular protein expression, which can promote inflammatory changes in dental pulp tissues. However, the mechanism of LPS-induced pulpitis is still unclear. Material/Methods: In this study, dental pulp stem cells (DPSCs) were separated and cultured from rat dental pulp tissues; then, LPS was administered to induce inflammation and activate the TLR4 pathway. Results: It was found that miR-506 was upregulated following LPS treatment in DPSCs. The inhibition of miR-506 in LPS-treated DPSCs led to attenuated inflammation and deactivation of the TLR4 pathway. Furthermore, the bioinformatic analysis and dual-luciferase reporter gene assay indicated that miR-506 could target the 3'-UTR of sirtuin 1 (SIRT1). Additionally, SIRT1 decreased in LPS-treated DPSCs, and miR-506 transfection resulted in SIRT1 upregulation. SIRT1 overexpression showed a similar inhibitory effect as that of miR-506 downregulation on inflammation and TLR4 activation in DPSCs. Conclusions: In brief, miR-506 can protect dental pulp in LPS-induced inflammation by inhibiting the SIRT1-mediated TLR4 pathway.

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“…This occurs in healthy dental pulp, and apoptotic cells are more numerous in the crown, mostly in a sub-odontoblastic locations, contributing to regulation of the pulp cell population 5 . Apoptosis of DPCs was reported to be induced by the resin monomer Bis-GMA, 6 lipopolysaccharides, 7 or some capping agents during pulp wound healing after cavity preparation 8 , 9 . Hydrogen peroxide (H 2 O 2 ), a reactive oxygen species (ROS), with a greater lifetime than other intracellular ROS, can easily diffuse across membranes and was proposed to be the predominant ROS involved in cell functions 10 .…”

Section: Introductionmentioning

confidence: 99%

Melatonin enhances hydrogen peroxide-induced apoptosis in human dental pulp cells

Deng

Liu

Zhang

et al. 2019

Journal of Dental Sciences

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Background/purposeMelatonin, at physiological concentrations, was previously found to inhibit proliferation and promote odontogenic differentiation in human dental pulp cells (hDPCs), but its effect on apoptosis is unclear. Our study aimed to investigate the effect of melatonin on the H2O2-mediated viability reduction and apoptosis in hDPCs.Materials and methodshDPCs were treated with H2O2 (0, 250, 500, 1000 μmol/L), melatonin (0, 10−12, 10−10, 10−8 mol/L), and melatonin with H2O2 for 24 h. CCK-8 assays were performed to evaluate cell viability. Apoptosis was measured by DAPI and Annexin V/propidium iodide staining. Intracellular reactive oxygen species (ROS) were measured by CellROX® staining and mitochondrial membrane potential (ΔΨm) was examined by JC-1 staining.ResultsH2O2 obviously decreased the viability of hDPCs in a concentration-dependent manner and melatonin alone also reduced viability by 16–20%. Melatonin was also found to enhance H2O2-induced toxicity in a concentration-dependent manner, and the highest physiological concentration of melatonin (10−8 mol/L) had the most obvious effect (P < 0.001). Treating H2O2-exposed hDPCs with melatonin significantly increased the ratio of apoptotic cells with condensed and deformed nuclei (P < 0.001), as well as the percentage of Annexin V-positive cells (P < 0.01). Furthermore, melatonin significantly increased intracellular ROS levels and induced the loss of ΔΨm in H2O2-exposed cells (P < 0.05).ConclusionOur results indicate that melatonin, at physiological concentrations, can enhance H2O2-induced apoptosis in hDPCs and increase H2O2-mediated ROS production and ΔΨm loss. Further studies are needed to investigate whether melatonin targets the mitochondrial death pathway during the process.

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Protective effects of SIRT6 against lipopolysaccharide (LPS) are mediated by deacetylation of Ku70

Cited by 15 publications

References 20 publications

The Roles of Sirtuin Family Proteins in Cancer Progression

The Roles of Sirtuin Family Proteins in Cancer Progression

MicroRNA-506 Is Involved in Regulation of the Occurrence of Lipopolysaccharides (LPS)-Induced Pulpitis by Sirtuin 1 (SIRT1)

Melatonin enhances hydrogen peroxide-induced apoptosis in human dental pulp cells

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