Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Tavares, Denise Crispim; Cecchi, Andr�a O.; Antunes, Lusânia Maria Greggi; Takahashi, Catarina Satie

doi:10.1002/(sici)1520-6866(1998)18:4<153::aid-tcm1>3.0.co;2-p

Cited by 37 publications

(18 citation statements)

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“…It is reported as a metabolically active molecule for a free radical state that interacts with molecular oxygen to generate superoxide radicals. (27) The results of the present study demonstrated a significant decrease in the frequency of micronuclei in polychromatic erythrocytes, which corroborates the studies of Sagrillo et al, (19) and de Souza Filho, (20) which recognized the protective potential of tucumã in cells submitted to oxidative stress, which is a producer of free radicals. The results obtained in the evaluations of the treatment groups of this study that used A. a. associated with DXR, to test the antigenotoxic effect, indicated a significant reduction of 36.57 ± 1.74% for treatments of 24 hours and 65.18 ± 1, 29% for those of 48 hours.…”

Section: Discussionsupporting

confidence: 92%

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

et al. 2017

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Objective: To obtain the oil of Astrocaryum aculeatum (A.a), and evaluate its genotoxicity/antigenotoxicity activities using the micronucleus test in peripheral blood cells. Methods: The oil of Astrocaryum aculeatum was obtained by hydraulic pressing. The animals used were healthy Swiss male mice, at 6-7 weeks of age; there were six per group. The genotoxic and antigenotoxic activity of concentrations were 500, 1,000 and 2,000 mg/kg per 0.5 mL (oral), followed or not followed by intraperitoneal injection of doxorubicin (0.3 mL-15 mg/kg by body weight), in addition to a negative group (water) and dimethyl sulfoxide (600 μL). Peripheral blood samples were collected 24h and 48h after treatment. Results: A statistically significant reduction was identified in the frequency of micronuclei in polychromatic cells ranging from 34.72% to 38.19% for 24-hour treatments, and from 63.70% to 66.12% for 48 hour. Conclusion: The fixed oil of tucumã presented antigenotoxic potential for the concentrations used in acute treatments. ResumoObjetivo: Obter o óleo do Astrocaryum aculeatum (A.a) e avaliar a genotoxidade/antigenotoxidade pelo teste do micronúcleo em células do sangue periférico. Métodos: O óleo da A.a foi obtido por prensagem hidráulica. Os animais foram camundongos Swiss, machos e saudáveis com 6-7 semanas de idade, 6 por grupo. Teste genotóxico e antigenotóxico as concentrações foram de 500, 1.000 e 2.000 mg/kg por 0,5 mL (via oral), seguidas ou não de injeção intraperitoneal de doxorrubicina (0,3mL -15 mg/kg por peso corporal), além do grupo negativo (água) e dimetilsufóxido (600 µL). As amostras de sangue periférico foram coletadas 24h e 48h após o tratamento. Resultados: Houve redução estatisticamente significativa na frequência de micronúcleos em células policromáticas que variou de 34,72% à 38,19% para os tratamentos de 24h, e de 63,70 à 66,12% para os de 48h. Conclusão: O óleo fixo do tucumã apresentou potencial antigenotóxico para as concentrações em tratamentos agudos.

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Section: Discussionsupporting

confidence: 92%

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

et al. 2017

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“…In contrast, there was no increase in micronucleus polychromatic erythrocytes frequency in either the MCD1or MCD2 group compared to control group. The significant increase in micronucleus polychromatic erythrocytes frequency in the group of doxorubicin-treated animals agrees with literature data [28,29], which show that a 24-hr sampling time after treatment with a single dose of doxorubicin is the optimal time for inducing mutagenicity [29]. The data obtained here by the comet and micronucleus tests for the MCD2 group were controversial.…”

Section: Experimental Groupssupporting

confidence: 89%

“…The comet assay usually detects more defects than the micronucleus test. Positivity to the comet and micronucleus tests is due to different mechanisms; the micronucleus test detects injuries that survive at least one mitotic cycle, while the comet assay identifies reparable injuries or alkali-label, facts that explain the positive result obtained with the comet test and the negative result obtained with the micronucleus test [29].…”

Section: Experimental Groupsmentioning

confidence: 99%

Progression of Lipid Peroxidation Measured as Thiobarbituric Acid Reactive Substances, Damage to DNA and Histopathological Changes in the Liver of Rats Subjected to a Methionine–Choline‐Deficient Diet

Jordão

Zanutto

Domenici

et al. 2009

Basic Clin Pharma Tox

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Methionine-choline-deficient diet represents a model for the study of the pathogenesis of steatohepatitis. Male rats were divided into three groups, the first group receiving a control diet and the other two groups receiving a methioninecholine-deficient diet for 1 month (MCD1) and for 2 months (MCD2), respectively. The livers of the animals were collected for the determination of vitamin E, thiobarbituric acid reactive substances (TBARS), GSH concentration, DNA damages, and for histopathological evaluation. The hepatic TBARS and GSH content was higher (P < 0.05) in the groups receiving the experimental diet (MCD1 and MCD2) compared to control diet, and hepatic vitamin E concentration differed (P < 0.05) between the MCD1 and MCD2 groups, with the MCD2 group presenting a lower concentration. Damage to hepatocyte DNA was greater (P < 0.05) in the MCD2 group (262.80 DNA injuries/100 hepatocytes) compared to MCD1 (136.4 DNA injuries/100 hepatocytes) and control diet (115.83 DNA injuries/100 hepatocytes). Liver histopathological evaluation showed that steatosis, present in experimental groups was micro-and macro-vesicular and concentrated around the centrolobular vein, zone 3, with preservation of the portal space. The inflammatory infiltrate was predominantly periductal and the steatosis and inflammatory infiltrate was similar in the MCD1 and MCD2 groups, although the presence of Mallory bodies was greater in the MCD2 group. The study describes the contribution of a methioninecholine-deficient diet to the progression of steatosis, lipid peroxidation and hepatic DNA damage in rats, serving as a point of reflection about the role of these nutrients in the western diet and the elevated non-alcoholic steatohepatitis rates in humans.

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“…In mouse bone marrow cells treated with the mitomycin or cyclophosphamide, VC decreased the frequency of sister-chromatid exchanges induced by these drugs (Krishna et al, 1986). Similar protection was obtained in rat bone marrow cells treated with VC or olive oil in combination with the antitumoral drug doxorubicin (Antunes and Takahashi, 1998, and in press;Tavares et al, 1998). The reduction in chromosomal damage observed with VC suggests a selection for lethally injured cells or by the protecting effect of VC.…”

Section: Resultssupporting

confidence: 52%

Anticlastogenic effect of vitamin C on cisplatin in vivo

1999

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The ability of vitamin C (VC) to protect against the clastogenic action of the chemotherapeutic agent cisplatin (DDP, cis-diamminedichloroplatinun II) in rat bone marrow cells was evaluated. DDP was administered to Wistar rats either alone or after treatment with VC. The rats were treated with VC (50, 100 or 200 mg/kg body weight) by gavage 10 min before the administration of DDP (5 mg/kg body weight, ip) and then sacrificed 24 h after treatment. VC significantly reduced (by about 70%) the clastogenicity of DDP in rat bone marrow cells. The antioxidant action of VC presumably modulates the clastogenic action of DDP.
Neste estudo foi avaliado o efeito protetor da vitamina C (VC) sobre a ação clastogênica da cisplatina (DDP) em células da medula óssea de ratos. A DDP, um agente quimioterápico, foi injetada em ratos Wistar sozinha ou após o tratamento com a VC. Os animais foram tratados com VC (50, 100 or 200 mg/kg de peso corporal) por gavagem 10 min antes da administração intraperitoneal de DDP (5 mg/kg de peso corporal). Os resultados obtidos mostraram que a VC foi efetiva na redução estatisticamente significativa da clastogenicidade da DDP em ratos Wistar (aproximadamente 70%). O efeito antioxidante da VC foi proposto para explicar a modulação da ação clastogênica da DDP

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Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells

Cited by 37 publications

References 50 publications

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

Efeito da Astrocaryum aculeatum (Tucumã) na toxicidade da Doxorrubicina: modelo experimental in vivo

Progression of Lipid Peroxidation Measured as Thiobarbituric Acid Reactive Substances, Damage to DNA and Histopathological Changes in the Liver of Rats Subjected to a Methionine–Choline‐Deficient Diet

Anticlastogenic effect of vitamin C on cisplatin in vivo

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