Protective effects of the hydroalcoholic extract of Fumaria parviflora on testicular injury induced by torsion/detorsion in adult rats

Abstract

This study was designed to determine the effects of daily oral administration (250 mg/kg) of the hydroalcoholic extract of Fumaria parviflora (FP) for 14 days on the sperm parameters, oxidative stress parameters, serum testosterone levels, expression of Bax and Bcl-2 genes, and apoptosis index of germ cells after testicular torsion-detorsion (ischaemia-reperfusion, IR) injury model in rats. Twenty-eight adult male Wistar rats were divided randomly into four groups of seven each: sham operation, torsion-detorsion (TD), TD plus the hydroalcoholic extract FP (TDFP) and only FP without TD application (FP). Testicular torsion was created by rotating the left testis 720° in a counterclockwise direction; then, after 4 hr, detorsion was performed. The Johnson's score, mean seminiferous tubule diameter (MSTD) and height (thickness) of seminiferous tubule epithelium (HST) were significantly increased in TDFP and FP groups as compared to TD group. The gene expression of Bcl-2, level of serum testosterone hormone and antioxidant parameters-GPx and SOD-were significantly higher in TDFP and FP groups than TD group. The index of apoptosis, the gene expression of Bax and the level of MDA were significantly higher in TD group than TDFP and FP groups. Therefore, F. parviflora could decrease oxidative stress induced by testicular torsion-detorsion.

The ameliorative effect of Galega officinalis Extract on Histological Damages, Oxidative Stress Induced by Torsion-Detorsion in Adult Rats’ Ovarian

“…The reproductive life of women or even thereafter can be affected by oxidative stress (5,6,15,16). Oxidative stress has been considered as an inconstancy between the generation of ROS and antioxidant defense capability of the body 122 (4)(5)(6)(7)9,14,15,17). The function of ROS has a similarity to the double-edged sword; one of their activities involves in body physiological processes as key signal molecules, but ROS have the main role in pathological damage in the reproductive system (15,16,18).…”

“…The level of malondialdehyde (MDA) in plasma was assessed based our previous studies (5,6,14). However, 0.2 mL of serum was poured into a microtube which had incubated with 3 mL glacial acetic acid.…”

“…After boiling stage (15 minutes), the absorbance of the product was read at 532 nm. Moreover, the levels of superoxide dismutase (SOD) and GSH peroxidase (GPx) in the plasma were measured according to the manufacturer's instructions (Randox, and Ransod, UK; sensitivity <S1 standard value) using an Elisa reader device (Antus) (4,5).…”

“…Accurate diagnosis and management of ovarian torsion is important, especially for women who are in childbirth age (2,3) Detorsion or opening the twist is one of the interventions that are performed to prevent ovarian tissue loss (3). Torsion followed by detorsion or ischemia-reperfusion is a pathophysiologic event that firstly results to reduction of oxygen in ovarian tissue (ischemia), then, after detorsion (reperfusion), results to elevating inflammatory cytokines that lead to in injury of endothelial cells of vessel and microcirculation disorders that are mostly responsible for the damage to the ovary tissue (3)(4)(5)(6). On the other hand, the ischemia-reperfusion damage led to over generation of reactive oxygen species (ROS) such as hydroxyl free radical species, hydrogen peroxide (H2O2), and superoxide (O 2 -) (3)(4)(5)(6).…”

Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM‐1 and VEGF‐A

“…The most valuable predictors of testicular injury are both of the degree and the period of torsion. The testicular rescue rate is nearly 90% when detorsion was done within 6 hr from starting of torsion and it slumped up to 10% after 24 hr, so emergency management is required (Shokoohi et al, ).…”