Protective effects of the postbiotic Lactobacillus plantarum MD35 on bone loss in an ovariectomized mice model

Abstract

Postmenopausal osteoporosis is caused by estrogen deficiency; it impairs the homeostatic balance of the bone, resulting in bone loss owing to increased osteoclastic resorption without a corresponding increase in osteoblastic activity. Postbiotics have several pharmaceutical properties, including anti-obesity, anti-diabetic, anti-inflammatory, and anti-osteoporotic activities. However, the beneficial effects of postbiotic MD35 of Lactobacillus plantarum on bone have not been studied. In this study, we demonstrated that the postbiotic Lactobacillus plantarum MD35, isolated from young radish water kimchi influenced osteoclast differentiation in mouse bone marrow-derived macrophage (BMM) culture. In addition, it was effective in estrogen deficiency-induced bone loss in ovariectomized (OVX) mice, an animal model of postmenopausal osteoporosis. In BMM cells, postbiotic MD35 inhibited the receptor activator of nuclear factor-kappa B of NF-κB ligand (RANKL)-induced osteoclast differentiation by significantly suppressing resorption activity and downregulating the expression of RANKL-mediated osteoclast-related genes; this was achieved by attenuating the phosphorylation of extracellular signal-related kinase. In the animal model, the oral administration of postbiotic MD35 remarkably improved OVX-induced trabecular bone loss and alleviated the destruction of femoral plate growth. Therefore, postbiotic MD35 could be a potential therapeutic candidate for postmenopausal osteoporosis by suppressing osteoclastogenesis through the regulation of osteoclast-related molecular mechanisms.

New insights into dairy management and the prevention and treatment of osteoporosis: The shift from single nutrient to dairy matrix effects—A review

“…For instance, the supernatant of Ligilactobacillus salivarius MG4265 significantly inhibited the activity of tartrate‐resistant acid phosphatase (TRAP), which is a marker of osteoclast differentiation and maturation (Jung et al., 2021). L. plantarum cell lysate inhibits RANKL‐induced osteoclast differentiation and downregulates the expression of osteoclast‐associated genes, thereby reducing bone resorption activity (Myeong et al., 2023). During Lactobacillus cultivation, the bacterial components are capable of naturally enriching calcium ions, acting as calcium delivery carriers, and promoting calcium ion adsorption and conversion.…”

Assessment of Multifunctional Activity of a Postbiotic Preparation Derived from <em>Lactobacillus paracasei</em> Postbio-P6

“…Especially in recent years, there has been growing evidence that postbiotics are useful tools in the fight against human diseases, and the focus of current research is gradually shifting from live probiotics to the postbiotics. For instance, in vitro and in vivo experiments have shown that postbiotics can play a role in boosting immunity [7], preventing colon cancer [8], maintaining oral health [9], preventing osteoporosis [10]and improving allergies [11]. Multifunctional active postbiotics are preferred over probiotics because of its safety and stability, reduced risk of microbial translocation, infection or enhanced inflammatory response [12].…”