Protective effects of thymoquinone and methotrexate on the renal injury in collagen-induced arthritis

Abstract

The goal of this investigation was to study the protective effects of thymoquinone (TQ) and methotrexate (MTX) on collagen-induced arthritis (CIA) in rats. On day 0 under ether anesthesia, the experimental groups were immunized with 0.5 mg native chick collagen II (CII) solubilized in 0.1 M acetic acid and emulsified in Freund's incomplete adjuvant. Control rats were gavaged with vehicle, whereas CII was administered intradermally. In addition, arthritis treated with TQ group received TQ (10 mg kg(-1) bw by gavage once a week for 3 weeks starting on day 0); and arthritis treated with MTX group received MTX (MTX was suspended in corn oil and administered by gavage at 1 mg kg (-1) bw once a week for 3 weeks starting on day 0). A significant decrease in the incidence and severity of arthritis by clinical and radiographic assessments was found in recipients of therapy, compared with that of controls. The MTX treatment significantly (P<0.01) decreased the elevated serum NO, urea and creatinine in arthritic rats. Likewise, TQ treatment was also able to reduce significantly (P<0.05) serum NO, urea and creatinine levels, but to lesser extent than MTX. The histopathologic abnormalities are consistent with the hydropic epithelial cell degenerations and moderate tubular dilatation in the some proximal and distal tubules. The severity of the degenerative changes in most of the shrunken glomerules and vascular congestion were also observed in arthritic animals. Preventive treatment of TQ and especially MTX significantly inhibited kidney dysfunction and this histopathologic alterations. These studies indicate that TQ can be used similar to MTX as a safe and effective therapy for CIA and may be useful in the treatment of rheumatoid arthritis.

Characterization of N-(Adamantan-1-ylmethyl)-5-[(3 R-aminopyrrolidin-1-yl)methyl]-2-chloro-benzamide, a P2X7 Antagonist in Animal Models of Pain and Inflammation

“…This is illustrated by the fact that methotrexate did not produce a significant effect when dosed in a similar therapeutic dosing paradigm even though methotrexate has clear therapeutic benefit in the clinic. It should be noted that methotrexate administered as a prophylactic exhibits significant efficacy in a CIA model (Budancamanak et al, 2006). This profile contrasts with the strong therapeutic effect seen here with dexamethasone, a frank immunosuppressant, and suggests that P2X 7 antagonism may have an effect more similar to methotrexate.…”

Methotrexate ameliorates pristane-induced arthritis by decreasing IFN-γ and IL-17A expressions

“…NC: normal control group; D: disease group; PL: BSO prevention low dose group; PH: BSO prevention high dose group; TL: BSO treatment low dose group; TH: BSO treatment high dose group; MTX: MTX treatment group BSO groups had declining trends of IL-17A, but no effect on IL-4 and IFN-γ expressions. Budancamanak et al (2006) and Tekeoglu et al (2006) reported that TQ had a treatment effect on adjuvant induced or collagen induced arthritis rat models. This observation may be caused by the use of different arthritis models and different medicinal components.…”

Antioxidative and Anti-inflammatory Effect of Thymoquinone in an Acute <b><i>Pseudomonas</i></b> Prostatitis Rat Model

“…We suggest that the decrease in GPX activity might be due to partial inhibition of lipid peroxidation and the consequent decrease in the reduction of H 2 O 2 . TQ has been proven to possess a potent antioxidant effect in terms of scavenging properties on free radicals such as superoxide anion [8,9,10,15,38]. A number of studies have demonstrated beneficial effects of various antioxidants and their free radical scavenging property in prostatitis [38,39,40,41].…”

“…The seeds of N. sativa have long been used in the Middle and Far East as a traditional medicine for a wide range of illnesses. TQ is a bioflavonoid with antioxidant, anti-inflammatory, neuroprotective, antiallergenic, antiviral, antidiabetic, and anticarcinogenic properties [8,9,10]. TQ may have a protective effect against inflammation, membrane lipid peroxidation, and infection-induced prostate toxicity.…”