Protective effects of thymoquinone on experimental testicular ischaemia-reperfusion injury: an apoptotic, proliferative and biochemical study

Erboğa, Mustafa; Aktaş, Cevat; Kurt, Ömer; Uygur, Ramazan; Çağlar, Veli; Turan, Bünyamin Cüneyt; Topçu, Birol; Erboğa, Zeynep Fidanol; Gürel, Ahmet; Ozen, Oguz Aslan

doi:10.1111/and.12436

Cited by 23 publications

(19 citation statements)

References 48 publications

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“…For histopathological examination, MTBS method was utilised for the evaluation of seminiferous injuries and MSTD was performed for measuring the seminiferous tubules diameter, both of which were frequently used in testicular damage models for histopathological examinations (Erboga et al., ; Kanter, Aktas, & Erboga, ). In our study, both MTBS and MSTD values were decreased in ethanol group.…”

Section: Discussionmentioning

confidence: 99%

The protective effects ofArctium lappaL. Extract on testicular injuries induced by ethanol in rats

et al. 2018

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The aim of this study is to assess the antioxidant activity and also protective effects of Arctium lappa L. (AL) extract on ethanol-induced testicular injuries in rats. In addition, the total phenol and flavonoid contents of the methanolic extracts were analysed and the antioxidant activity of the extract was investigated. The studied extract possessed strong antioxidant activity (IC ; 0.113 ± 0.007 mg/ml) and exhibited more active than the ascorbic acid (IC ; 0.142 ± 0.002 mg/ml). The histological examinations revealed that the co-administration of ethanol and AL extract inhibits the testicular injuries, alterations in superoxide dismutase (SOD) activity, and H O and malondialdehyde (MDA) levels were observed in ethanol-treated rats in comparison to the control group. The obtained results demonstrated strong pharmaceutical activities of AL and its potential capacity to be developed as a new health-care supplement against ROS-related substances.

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Section: Discussionmentioning

confidence: 99%

The protective effects ofArctium lappaL. Extract on testicular injuries induced by ethanol in rats

et al. 2018

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“…The favourable effect on the sperm parameters was due to the excellent ability of N. sativa to inactivate free radicals and prevent oxidative stress in the semen of infertile subjects (Kolahdooz et al, 2014). To support this assumption, thymoquinone, the major pharmacologically active component of N. sativa oil was also tested in different animal models and was found to re-establish spermatogenesis and protect against testicular ischaemia-reperfusion injury and chemically induced testicular injury through an antioxidant mechanism (Erboga et al, 2016;Kanter, 2011;Salahshoor, Haghjoo, Roshankhah, Makalani, & Jalili, 2018). Apparently, there are yet no clinical trials conducted in infertile men treated with thymoquinone except the only one study that evaluated the effects of N. sativa oil in childless men (Kolahdooz et al, 2014).…”

Section: Ni G Ell a Sativamentioning

confidence: 99%

Plants in the management of male infertility

Onuah

Singh

2020

Andrologia

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This review attempts to collate existing data and provide the perspectives for future studies on the effects of plants on the male gonads. For many of these medicinal plants such as Lepidium meyenii, Rupus coreanus, Tribulus terrestres, Panax ginseng, Petasites japonicas, Apium graveolens, Eurycoma longifólia, Pedalium murex, Corchorus depressus, Mucuna pruriens, Astragalus membranaceus, Nigella sativa, Crataegus monogyna, Fagara tessmannii, Phaleria macrocarpa, Anacyclus pyrethrum, Cynomorium songaricum and Morinda officinalis, the mechanism of actions of their active principles and crude extracts has been shown in both laboratory animals, in vitro, and human studies, and includes their antioxidant, anti‐inflammatory, spermatogenesis‐inducing, aphrodisiac, smooth muscle relaxing and androgenic properties. Several active chemical leads including glucosinolates, anthocyanins, protodioscin, ginsenosides, sesquiterpenes, phyto‐oestrogens, quassinoids, diosgenin, thymoquinone, proanthocyanidins and bajijiasu isolated from these plants are known to have target effects on the testis, but efforts have been limited in their application at the clinical level. There still appear to be many more extracts of medicinal plants that have not been characterised to determine the phytochemicals unique to them that have target effects on the gonads. Further, collaborative efforts at isolating pro‐drug candidates from medicinal plants for studies at the molecular, cellular and clinical level towards elucidating their mechanisms of action on the testes are therefore warranted in the light of the current male fertility crisis.

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“…catalase (CAT), glutathione peroxidase (Gpx), superoxide dismutase (SOD), and nitrite nitrate (NIT)] and decreasing lipid peroxidation (Kanter et al ., ; Awad et al ., ). Over the last decade, studies of I/R injury have reported that TQ reverses the toxic effects ROS in the affected organ (Bayrak et al ., ; Erboga et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Comparison of combined antioxidants and thymoquinone in the prevention of testis ischemia – reperfusion injury

et al. 2016

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SUMMARYWe aimed to compare the preventive effects of combined antioxidants (CA1, 2) with a single antioxidant drug (thymoquinone; TQ) on experimental testis Ischemia/Reperfusion (I/R) injury. Thirty-five adult male Wistar rats were divided into five groups of seven rats each: control, testis I/R, testis I/R + CA1, testis I/R + CA2, and testis I/R + TQ. After 1 h of testicular ischemia, reperfusion was achieved by detorsion for 4 h. Antioxidants were intraperitoneally administered for 30 min prior to reperfusion. All rats were sacrificed 4 h after reperfusion to evaluate the tissue levels of malondialdehyde (MDA) and total antioxidant status (TAS) and the immunohistochemical evaluation of tissue inducible and endothelial nitric acid synthase (iNOS, eNOS) and apoptosis protease-activating factor 1 (APAF-1). MDA levels were lower and TAS values were higher in the I/R + antioxidant groups than in the I/R group (p < 0.05). iNOS and eNOS levels in the I/R + antioxidant groups were also lower than those in the I/R group (p < 0.05). There were no significant differences between the CA groups and the TQ group according to aforementioned parameters. In addition, tissue APAF-1 values were significantly higher in the I/R group than in the other groups. However, there was a significant difference between the TQ and CA groups in APAF-1 levels, which were highest in the TQ group (p < 0.05). Although TQ alone increased TAS values and reduced tissue iNOS and eNOS levels, combined antioxidant treatment may more effectively reduce apoptosis and increase preventive effects in testis I/R injury.

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Protective effects of thymoquinone on experimental testicular ischaemia-reperfusion injury: an apoptotic, proliferative and biochemical study

Cited by 23 publications

References 48 publications

The protective effects ofArctium lappaL. Extract on testicular injuries induced by ethanol in rats

The protective effects ofArctium lappaL. Extract on testicular injuries induced by ethanol in rats

Plants in the management of male infertility

Comparison of combined antioxidants and thymoquinone in the prevention of testis ischemia – reperfusion injury

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