Protective effects of various dosage of Curcumin against morphine induced apoptosis and oxidative stress in rat isolated hippocampus

Abstract

These findings have demonstrated that Curcumin can act as an antioxidant and antiapoptotic agent against damage induced by morphine dependence.

Effects of chronic treatment with methylphenidate on oxidative stress and inflammation in hippocampus of adult rats

“…Then in separate process 10, 15, 20, 25 and 30 μl of the mentioned protein extract (homogenized cell solutions) added to multiple wells and Bradford reagent was added to each well. Density of colors of all wells were read by plate reader at 630 nm , finally by using of mentioned standard curve, protein quantity of unknown protein extracts were obtained These homogenized cell solutions were analyzed for the measurement of oxidative stress and inflammatory markers [19,20].…”

The neuroprotective effect of lithium against high dose methylphenidate: Possible role of BDNF

“…The homogenized cell solutions were analyzed for measurement of oxidative stress and apoptosis factors (Motaghinejad et al, 2015a, Motaghinejad et al, 2015b, Motaghinejad and Motevalian, 2016.…”

“…The quantity of this compound was calculated using an extension coefficient of 165 mM -1 cm -1 at 530 nm. Results were expressed as nmol/mg protein (Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b, Serteser et al, 2002.…”

“…The reduced and oxidized forms of glutathione (GSH and GSSG, respectively) were measured using two different kits: Bioxytech GPx-340 and Bioxytech GR-340 (Oxis Research, USA) and the results were reported as nmol/mg protein (Capela et al, 2007, Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b was added to each well. The further incubation was carried out for 1 h at room temperature.…”

“…The absorbance was detected at 450 nm and the findings were expressed as U/ml/mg protein(Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b.<H3>2.5.4 Determination of glutathione peroxidase (GPx) activityThe activity of GPx was measured by Bioxytec GPx-340 kits (Oxis Research, USA). Results were reported as mU/mg protein(Hsieh et al, 2015, Motaghinejad, Karimian, 2015a, Motaghinejad, Karimian, 2015b.<H3>2.5.5 IL-1β and TNF-α measurementConcentrations of IL-1β and TNF-α in supernatant of hippocampal cells were measured using a commercially available ELISA kit (Genzyme Diagnostics, Cambridge, U.S.A.). Briefly, 96 well microtitre plates (Nunc) were coated with sheep anti-rat IL-1β and TNF-α polyclonal antibodies (2 mg/ml in bicarbonate coating buffer; 0.1 M NaHCO3, 0.1 M NaCl, pH 8.2, for 20 h at 48 °C), then washed three times with washing buffer (0.5 M NaCl, 2.5 mM NaH2PO4, 7.5 mM Na2HPO4, 0.1% Tween 20, pH 7.2).…”