Protective effects of vitamin C and selenium supplementation on methomyl-induced tissue oxidative stress in adult rats

Abstract

Methomyl (MET) is used worldwide in agriculture and health programs. Besides its advantages in the agriculture, it causes several toxic effects. The objective of this study was to examine the potential ability of vitamin C and/or selenium (Se), to alleviate the oxidative damage parameters, against MET-induced changes in blood biochemical markers and oxidative damage in liver and kidney of male Wistar rats. The animals were randomized into five groups of eight each: group I served as control rats; group II received MET (8 mg/kg body weight (BW)) in drinking water; group III received both MET and vitamin C (200 mg/kg BW; by intraperitoneal injection); group IV received both MET and Se (0.6 mg/100 g BW). Animals of group V were treated with MET, vitamin C and Se. A significant increase in the levels of hepatic markers enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and lactate dehydrogenase) was determined. Furthermore, renal markers such as urea and creatinine were increased in MET-treated rats. Additionally, serum cholesterol and triglycerides were significantly enhanced. Exposure of rats to MET caused significant increase in malondialdehyde levels, thus causing a drastic alteration in antioxidant defense system, particularly in the activities of catalase and glutathione-S-transferase and glutathione peroxidase. However, simultaneous supplementation with vitamin C and Se restored these parameters partially. In conclusion, the results of the current study revealed that MET-induced toxicity caused perturbations of some biochemical parameters, lipid peroxidation and alterations in the antioxidant enzymes in liver and kidney homogenates. Administration of vitamin C and Se exhibited protective effect by inhibiting MET-induced toxicity in liver and kidney.

Methidathion-induced hematological, biochemical and hepatohistological alterations in rat: Ameliorative effects of selenium and zinc

“…MDA is a marker of membrane LPO resulting from the interaction of ROS and the cellular membrane (Guney et al, 2007a;2007b). In our previous studies, pesticides such as diazinon and methomyl Djeffal et al, 2012) caused a significant increase in LPO in a manner similar to the present study and, in addition, treatment with a combination of Se and Zn after the administration of MD led to a significant decrease in LPO levels. The significant drop in GSH levels promoted by MD caused a reduction in the effectiveness of the antioxidant enzyme defense system, sensitizing the cells to ROS (Altuntas et al, 2002a).…”

Selenium Level and Dyslipidemia in Rural Elderly Chinese

“…A recent study reported a nonlinear dose-response relationship between selenium exposure and oxidative stress biomarkers suggesting that higher selenium levels increased oxidative stress [ 42 ]. In addition, there were also findings indicating that selenium can be identified in human lipoproteins [ 43 ] and experimental studies suggesting that selenium may play an important role in lipid peroxidation and lipoprotein metabolism [ 14 – 16 , 44 ].…”

“…As a key component of selenoproteins, selenium plays important roles against oxidative stress, which is also important to lipid metabolism [ 13 ]. Animal studies had indicated that selenium deficiency may modulate lipoprotein metabolism [ 14 – 16 ]. Selenium supplementation has also been reported to reduce non-HDL cholesterol in a randomized trial of elderly volunteers [ 17 ].…”

Antioxidant activity of selenium on bisphenol-induced apoptosis and testicular toxicity of rats

“…The human body has several mechanisms to counteract oxidative/nitrosative stress via the production of antioxidants (Kurutas, 2016). In particular, Selenium (Se) is an essential trace element for humans and animals (Tuormaa et al, 2000;Saito et al, 2003) that can prevent the formation of reactive metabolites induced by many toxicants (Atif et al, 2008;Djeffal et al, 2015). Selenium is well known for its effective antioxidant influence against unfavorable residues and chemicals; furthermore, Se decreases the risk organ injury, such as intestines, heart, lungs, and kidneys (Parízek, 1990;Benstoem et al, 2015) and it exhibits protective effects against cadmium-induced testicular toxicity .…”