Protective effects of vitamin D against injury in intestinal epithelium

Lee, Carol; Lau, Ethan; Chusilp, Sinobol; Filler, R M; Li, Bo; Zhu, Haitao; Yamoto, Masaya; Pierro, Agostino

doi:10.1007/s00383-019-04586-y

Cited by 36 publications

(26 citation statements)

References 30 publications

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“…In the present study, LPS-induced inflammation was inhibited by vitamin D ( Figure 1 ), which is consistent with the results of Lee et al, who showed that LPS-induced injury in cells was reversed and cell viability was increased by vitamin D treatment [ 29 ]. Moreover, Khare et al confirmed that treatment of A549 cells with vitamin D before infection and after infection did not alter cell viability that developed in the absence of vitamin D exposure [ 30 ].…”

Section: Discussionsupporting

confidence: 93%

Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

Gatera

Lesmana

Musfiroh

et al. 2021

Med Sci Monit Basic Res

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Background Studies have shown that lung inflammation affects lung function, with life-threatening results. Vitamin D may play an important role in inhibiting inflammatory cytokines. Vitamin D deficiency is related to several lung problems, including respiratory distress syndrome, alveolar inflammation, epithelial damage, and hypoxia. Few studies have evaluated the benefits of vitamin D in preventing inflammation in alveolar cells. Material/Methods We developed a cell inflammation model induced by lipopolysaccharide (LPS) treatment. The effects of vitamin D on LPS-induced inflammation in A549 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anti-inflammatory mechanism of vitamin D was evaluated using western blot analysis. Results Our results indicated that vitamin D promoted A549 cell survival following LPS-induced inflammation by downregulating nuclear factor nuclear factor kappa light chain enhancer of activated B cells, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-12. Conclusions Our results indicated that vitamin D has the potential to manage lung inflammation, although further studies are needed.

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Section: Discussionsupporting

confidence: 93%

Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

Gatera

Lesmana

Musfiroh

et al. 2021

Med Sci Monit Basic Res

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“…Multiple studies have shown that vitamin D plays a role in maintaining gut integrity and intestinal homeostasis between host and gut microbiota. Vitamin D signaling is shown to increase the viability of intestinal epithelial cells and and alleviate intestinal epithelial damages from bacterial lipopolysaccharide [ 43 , 44 ]. It promotes mucosal barrier function by enhancing the expression of intracellular pathogen recognition proteins and epithelial membrane junction proteins [ 45 , 46 ].…”

Section: Effects Of Vitamin D On Innate Immunitymentioning

confidence: 99%

Immunologic Effects of Vitamin D on Human Health and Disease

2020

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Vitamin D is responsible for regulation of calcium and phosphate metabolism and maintaining a healthy mineralized skeleton. It is also known as an immunomodulatory hormone. Experimental studies have shown that 1,25-dihydroxyvitamin D, the active form of vitamin D, exerts immunologic activities on multiple components of the innate and adaptive immune system as well as endothelial membrane stability. Association between low levels of serum 25-hydroxyvitamin D and increased risk of developing several immune-related diseases and disorders, including psoriasis, type 1 diabetes, multiple sclerosis, rheumatoid arthritis, tuberculosis, sepsis, respiratory infection, and COVID-19, has been observed. Accordingly, a number of clinical trials aiming to determine the efficacy of administration of vitamin D and its metabolites for treatment of these diseases have been conducted with variable outcomes. Interestingly, recent evidence suggests that some individuals might benefit from vitamin D more or less than others as high inter-individual difference in broad gene expression in human peripheral blood mononuclear cells in response to vitamin D supplementation has been observed. Although it is still debatable what level of serum 25-hydroxyvitamin D is optimal, it is advisable to increase vitamin D intake and have sensible sunlight exposure to maintain serum 25-hydroxyvitamin D at least 30 ng/mL (75 nmol/L), and preferably at 40–60 ng/mL (100–150 nmol/L) to achieve the optimal overall health benefits of vitamin D.

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“…In Caco-2 cells treated with LPS, emulating the barrier damage of necrotizing enterocolitis (NEC), the presence of 1,25-Dihydroxyvitamin D3 -active form of vit D-restored the expression and localization of TJ proteins and reverted LPSinduced down-regulated VDR expression (86). Likely, intestinal damage caused by LPS in IEC-18 line cells and organoids was improved after vit D treatment, restoring permeability and TJ (87). Similar findings have been reported in a model of alcoholic liver disease in Caco-2 challenged with ethanol (88).…”

Section: Vitaminsmentioning

confidence: 98%

Present and Future Therapeutic Approaches to Barrier Dysfunction

et al. 2021

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There is converging and increasing evidence, but also uncertainty, for the role of abnormal intestinal epithelial barrier function in the origin and development of a growing number of human gastrointestinal and extraintestinal inflammatory disorders, and their related complaints. Despite a vast literature addressing factors and mechanisms underlying changes in intestinal permeability in humans, and its connection to the appearance and severity of clinical symptoms, the ultimate link remains to be established in many cases. Accordingly, there are no directives or clinical guidelines related to the therapeutic management of intestinal permeability disorders that allow health professionals involved in the management of these patients to carry out a consensus treatment based on clinical evidence. Instead, there are multiple pseudoscientific approaches and commercial propaganda scattered on the internet that confuse those affected and health professionals and that often lack scientific rigor. Therefore, in this review we aim to shed light on the different therapeutic options, which include, among others, dietary management, nutraceuticals and medical devices, microbiota and drugs, and epigenetic and exosomes-manipulation, through an objective evaluation of the scientific publications in this field. Advances in the knowledge and management of intestinal permeability will sure enable better options of dealing with this group of common disorders to enhance quality of life of those affected.

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Protective effects of vitamin D against injury in intestinal epithelium

Cited by 36 publications

References 30 publications

Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

Vitamin D Inhibits Lipopolysaccharide (LPS)-Induced Inflammation in A549 Cells by Downregulating Inflammatory Cytokines

Immunologic Effects of Vitamin D on Human Health and Disease

Present and Future Therapeutic Approaches to Barrier Dysfunction

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