Protective effects of Vitamin E on CCl4-induced testicular toxicity in male rats

Abstract

The increased generation of free radicals plays an important role in testicular damage. The present study aimed to investigate the adverse effects of carbon tetrachloride (CCl 4 ) on the reproductive system of male rats as well as to examine whether Vitamin E (VE) is able to ameliorate these effects. The rats were equally divided into three groups: control, CCl 4 -treated, and CCl 4 + VE-treated groups. After 4 weeks of treatment, the decrease in body and testes weights, sperm parameters, and the decrease in serum levels of testosterone, luteinizing hormone, and folliclestimulating hormone of CCl 4 -treated rats were ameliorated by VE treatment. The co-administration of VE with CCl 4 significantly decreased the level of lipid peroxidation production (malondialdehyde) and increased the activity of anti-oxidant enzymes (superoxide dismutase and catalase) when compared with the CCl 4 group. Moreover, VE prevented CCl 4 -induced severe testicular histopathological lesions and deformities in spermatogenesis. The results demonstrate that VE augments the anti-oxidants' defense mechanism against CCl 4 -induced reproductive toxicity suggesting a therapeutic role in free radical-mediated infertility.

Carbon Tetrachloride-Induced Testicular Toxicity and Histopathological Alteration in Male Swiss Albino Mice

“…The significant reduction in body weight may be due to a decrease in food intake, which leads to a decrease in the body weight of the CCl4-treated group. The results of the current study were compatible with other studies [12,[21][22][23] which elucidated that CCl4 compounds caused a decline in the body weight of treated animals compared to the control.…”

Zinc loaded whey protein nanoparticles mitigate the oxidative stress and modulate antioxidative gene expression in testicular tissues in rats

“…The interstitial spaces also showed acidophilic exudates and congested blood vessels. Similar observations were reported by El-Faras et al [52] who found a marked degeneration of the spermatogenic layers and interlobular hemorrhage. Al-Olayan et al [48] reported a prominent inflammation, complete swallowing of seminiferous tubules and degenerated germ cells in the testis or rats treated with CCl 4 .…”

“…Actually, the decrease of antioxidant enzymes in the testicular tissue in CCl 4 -treated animals was reported previously [48] and indicated the decrease in the bioavailability of GSH due to the enhancement of lipid peroxidation and/or the inactivation of the antioxidant enzymes during oxidative stress and the accumulation of superoxide anion O 2 [49]. CCl 4 induced a significant decrease in serum testosterone level, which may be directly through the degeneration of Leydig cells due to the excessive oxidative stress [48] or indirectly through the stimulation of P450, which catalyses the production of estrogen from androgen [50,51] as was reported by several authors [52,53].…”

Adri̇ami̇si̇ni̇n Oluşturduğu Testi̇s Hasari Üzeri̇ne E Vi̇tami̇ni̇ Ve Selenyum’un Koruyucu Etki̇si̇ni̇n İncelenmesi̇

“…Vitamin E'nin bu koruyucu etkisinin, serbest radikalleri dog rudan su pu ru cu o zellig inden kaynaklanıyor olabileceg i ileri su ru lmu ştu r (30). Yapılan bir çalışmada Karbon tetrakloru r ve Vitamin E uygulanan sıçanların vu cut ve testis ag ırlıg ının kontrol grubu du zeyine çekildig i bildirilmiştir (31). Sodyum arsenit uygulamasına maruz kalan sıçanların testis dokusunda oluşan hasara karşı Vitamin E'nin seminifertu bu l çapını arttırarak, seminifertu bu l duvarını kalınlaştırarak ve tu bu l lu menindeki boşlug u azaltarak testislerin morfolojik go ru numu nde du zeltici etki sag ladıg ı go ru lmu ştu r (32) (31), dig er bir çalışmada ise Vitamin E'nin dioksinle oluşturulan testis hasarını du şu rdu g u ve azalan sperm sayısı, testosteron, LH ve FSH seviyelerini antagonize ettig i go sterilmiştir (33).…”