Protective effects of β‐casofensin, a bioactive peptide from bovine β‐casein, against indomethacin‐induced intestinal lesions in rats

Bessette, Claudine; Benoit, Bérengère; Sekkal, Soraya; Bruno, Jérémie; Estienne, Monique; Léonil, Joëlle; Ferrier, Laurent; Théodorou, Vassilia; Plaisancié, Pascale

doi:10.1002/mnfr.201500680

Cited by 36 publications

(19 citation statements)

References 37 publications

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“…e duodenal mucosal lesions were induced with indomethacin (IND) according to the method described by Bessette et al [19] with minor modifications. A total of 50 rats were randomly divided into five groups (each group consisting of 10 animals); the treatment schedule was as follows.…”

Section: Induction Of Duodenal Ulcermentioning

confidence: 99%

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Song

Zeng

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Background. Administration of nonsteroidal anti-inflammatory drugs (NSAIDs) often causes small intestinal ulcers in patients, but few effective drugs are currently available to manage such serious adverse events of NSAIDs. Li-Zhong decoction (LZD), a well-known traditional Chinese medicine (TCM) formula, is commonly prescribed for treatment of gastrointestinal diseases. The present study aimed to investigate the anti-ulcerogenic activity of LZD on indomethacin- (IND-) induced duodenal ulcer in rats. Mechanistic studies of action of LZD were focused on involvement of TLR-2/MyD88 signaling pathway. Methods. Fifty male Sprague-Dawley (SD) rats were randomly and evenly divided into five groups: normal control, ulcer control (IND, 25 mg/kg), IND + esomeprazole (ESO, 4.17 mg/kg), and IND + low and high doses of LZD (3.75 and 7.50 g/kg). Macroscopic and histopathological examinations were performed for evaluation of ulcer index (UI), curative index (CI), and microscopic score (MS). Levels of duodenal inflammatory biomarkers and cytoprotective mediators including interleukin-4 (IL-4), IL-10, tumor necrosis factor-α (TNF-α), and prostaglandin E2 (PGE2) were measured by ELISA. Expression levels of TLR-2 and MyD88 mRNA were assessed by qRT-PCR. The expression and distribution of TLR-2 and MyD88 proteins were analyzed by western blot and immunohistochemistry, respectively. Results. Gross and microscopic examinations of the IND-treated rats revealed severe duodenal hemorrhagic necrosis, inflammatory infiltration, villus destruction, and crypt abscess, while LZD-treated rats manifested these pathological events to a markedly lesser degree. LZD significantly decreased UI and MS, increased CI, preserved the integrity of the villus and crypt, and normalized the tissue architecture of the duodenum of rats. The elevated TNF-α levels in the IND-treated rats were markedly diminished in the LZD-treated rats, while lower levels of IL-4, IL-10, and PGE2 observed in IND-treated rats were significantly increased in LZD-treated rats. Interestingly, improvement of immune function in duodenal mucosa by reduction of mRNA and protein expression levels of TLR-2 and MyD88 was also observed in rats treated with LZD. Consistently, immunohistochemical analyses revealed a lower co-localization of TLR-2 and MyD88 proteins in the duodenal mucosa of LZD-treated rats as compared to the IND-induced rats. Conclusions. Our data demonstrate that LZD protects the duodenal mucosa from IND-caused lesions, which is at least partially attributable to the interaction of its potential cytoprotective and anti-inflammatory mechanisms together with enhancement of the mucosal immunity through TLR-2/MyD88 signaling pathway.

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Section: Induction Of Duodenal Ulcermentioning

confidence: 99%

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Song

Zeng

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Intestinal recovery, after a potential damage, includes several processes which contribute to repair the intestinal epithelial structure by reducing cell‐free gaps (Söderholm and Perdue, ; Bessette et al., ). Butyrate stimulated such processes in human and rats (Kinoshita et al., ; Dalmasso et al., ; Qiao et al., ).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary butyrate supplementation on intestinal integrity of heat‐stressed cockerels

Abdelqader

Abuajamieh

Hammad

et al. 2017

Animal Physiology Nutrition

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Butyrate modulates intestinal epithelial cell structure and function. Three hundred and sixty Lohmann LSL-Classic layer cockerels were used to investigate the effect of butyrate on heat stress-induced intestinal injury and intestinal integrity. The experiment was conducted from day 85 to 105 of age. The birds were divided randomly into three treatments: control, heat stress (HS), and heat stress provided with butyrate (HSB) at a level of 0.35 g/kg of diet. The control was reared at 21 ± 1 °C throughout the experiment. The HS and HSB treatments were exposed to a cyclic HS (35 ± 1 °C from 09:00 to 13:00 and 21 ± 1 °C from 13:00 to 09:00). Intestinal and mucosal weights, villus height, villus surface area (VSA), absorptive epithelial cell area and intestinal beneficial bacteria were lower in the HS treatment than in the other two treatments (p < 0.05). Heat-stressed cockerels exhibited the highest (p < 0.05) villi injury scores and serum endotoxin levels compared with the other treatments. Dietary inclusion of butyrate increased (p < 0.05) intestinal and mucosal weights, villus height, VSA, absorptive epithelial cell area and intestinal beneficial bacteria counts and reduced (p < 0.05) HS-induced injury in intestinal epithelia as well as intestinal permeability to endotoxin. In conclusion, dietary butyrate exerted protective effects against intestinal damage induced by HS and improved intestinal health and integrity.

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“…[93] Later on, a bioactive -casein f(94-123) peptide, termed as -casofensin, was found to protect against intestinal epithelial injury induced by both the drug indomethacin and neonatal maternal separation, improving the mucosal barrier status of treated rats. [94,95] In addition, the anti-inflammatory properties of caseinomacropeptide have been confirmed in some animal models of intestinal inflammation. [82] The short-term intake of soybean (2 g kg −1 body weight, daily during 7 days) has been found to increase the total antioxidant activity of healthy men and women, and diminish the advanced oxidation protein products in the female group.…”

Section: Oxidative Damage and Inflammationmentioning

confidence: 95%

“…[ 93 ] Later on, a bioactive β‐casein f(94‐123) peptide, termed as β‐casofensin, was found to protect against intestinal epithelial injury induced by both the drug indomethacin and neonatal maternal separation, improving the mucosal barrier status of treated rats. [ 94,95 ] In addition, the anti‐inflammatory properties of caseinomacropeptide have been confirmed in some animal models of intestinal inflammation. [ 82 ]…”

Section: Bioactivity Of Peptides Over Digestive Healthmentioning

confidence: 99%

Gastrointestinal Digestion of Food Proteins under the Effects of Released Bioactive Peptides on Digestive Health

Fernández-Tomé

Hernández-Ledesma

2020

Molecular Nutrition Food Res

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The gastrointestinal tract represents a specialized interface between the organism and the external environment. Because of its direct contact with lumen substances, the modulation of digestive functions by dietary substances is supported by a growing body of evidence. Food-derived bioactive peptides have demonstrated a plethora of activities in the organism with increasing interest toward their impact over the digestive system and related physiological effects. This review updates the biological effects of food proteins, specifically milk and soybean proteins, associated to gastrointestinal health and highlights the study of digestion products and released peptides, the identification of the active form/s, and the evaluation of the mechanisms of action underlying their relationship with the digestive cells and receptors. The approach toward the modifications that food proteins and peptides undergo during gastrointestinal digestion and their bioavailability is a crucial step for current investigations on the field. The recent literature on the regulation of digestive functions by peptides has been mostly considered in terms of their influence on gastrointestinal motility and signaling, oxidative damage and inflammation, and malignant cellular proliferation. A final section regarding the actual challenges and future perspectives in this scientific topic is critically discussed.

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Protective effects of β‐casofensin, a bioactive peptide from bovine β‐casein, against indomethacin‐induced intestinal lesions in rats

Abstract: β-casofensin exerts protective effects in indomethacin-induced enteritis through preservation of goblet cells and improvement in wound healing. β-casofensin could therefore become vital in nutritional programs for the prevention of intestinal diseases.

Cited by 36 publications

References 37 publications

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

Effects of dietary butyrate supplementation on intestinal integrity of heat‐stressed cockerels

Gastrointestinal Digestion of Food Proteins under the Effects of Released Bioactive Peptides on Digestive Health

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