Protective efficacy of mycobacterial 71 kilodalton cell wall associated protein using poly (DL-lactide-co-glycolide) microparticles as carrier vehicles

Dhiman, Neelam; Khuller, G. K.

doi:10.1007/bf02873068

Cited by 1 publication

(1 citation statement)

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“…Microparticles with entrapped 71-kDa protein were prepared by the double emulsion solvent evaporation technique as described previously (Dhiman and Khuller 1998) using 50 : 50 poly lactide-coglycolide (PLG) co-polymer (RG506, MW2500; Boehringer Ingellheim, Germany).…”

Section: Poly Lactide-coglycolide Microparticle Preparationmentioning

confidence: 99%

Immunoreactivity of peptides generated by limited proteolysis of 71-kDa cell wall protein of Mycobacterium tuberculosis H37Ra using PLG-microparticles

Dhiman

Khuller

2000

Lett Appl Microbiol

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Peptide mapping by limited proteolysis of a highly protective 71-kDa cell wall-associated protein of Mycobacterium tuberculosis H37Ra was carried out in order to identify key protective determinants within the native protein. The 71-kDa protein, which had an isoelectric point of 4.25, was digested into eight major bands at 48 h using trypsin and pepsin at equal enzyme to protein ratios (pH 5.5). The in vitro lymphocyte reactivity of individual peptides suggested P1, P2 and P5 to be significantly immunoreactive in mice immunized with native 71-kDa-polylactide-coglyeolide (PLG); however, the reactivity was significantly lower than that of the native 71-kDa protein. Immunization of mice with a pooled fraction (upper fraction-71 kDa) of more immunoreactive peptides (consisting of P1 and P2) did not further boost their immunoreactivity. However, P1 and P2 exhibited comparable or even higher lymphocyte proliferation in human tuberculous and control subjects. These data suggest distinct antigenic specificities in humans and mice and further substantiate the use of the 71-kDa protein or its peptides P1 and P2 as potential vaccine candidates for tuberculosis.

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Section: Poly Lactide-coglycolide Microparticle Preparationmentioning

confidence: 99%