Protective functions of methanol extract of Cucumis Melo (L. INODOROUS) seeds on Pyloric ligation induced gastric ulceration in male Wistar rats

Abstract

Accumulation of gastric acid and Reactive oxygen species (ROS) have been associated with development the of gastric ulceration in pyloric ligation, Cucumis melo Seeds is said to be possess a strong antioxidant. Thus this study evaluated the antiulcer activities of Methanol extract of Cucumis melo seeds on Pyloric ligation induced gastric ulceration in male Wistar rats. Thirty Male Wistar rats (n=5, 150-200g) rats were randomly groups in to five groups; A (control),B(Pyloric ligation induced gastric ulceration(PYL),C,D,E were administered with 50mg/kg,100mg/kg,200mg methanol extract of Cucumis melo seeds + PYL for 21 days prior to to pyloric ligation induction under ketamine anesthesia(60mg/kg). The animals were sacrifice after six hours, total gastric acidity was measured by titrimetric method, ulcer score and index was assessed, hematological parameters was measure using auto-analyzer while MDA,Catalase and protein was determined by spectrophotometry with histology analysis. Upon Pyloric ligation, the total gastric acidity, ulcer score ,index, lipid peroxidation(MDA level) was increased, while there was a decrease in the catalase, protein level and hematological parameters, with damages on the gastric mucosa . Cucumis melo seed extract reverses these changes induced by pyloric ligation. In conclusion, the gastric mucosa was disrupted as a result of accumulation acid, increases in oxidation after pyloric ligation with decrease in antioxidant and hematological parameters, methanol extract of Cucumis Melo provides a protection against the pyloric ligation induced gastric ulceration.