Protective Immunity against
            <i>Eimeria acervulina</i>
            following In Ovo Immunization with a Recombinant Subunit Vaccine and Cytokine Genes

Ding, Xicheng; Lillehoj, Hyun S.; Quiróz, Marco A.; Bevensee, Erich; Lillehoj, Erik P.

doi:10.1128/iai.72.12.6939-6944.2004

Cited by 104 publications

(54 citation statements)

References 49 publications

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“…Therefore, with the progress of molecular biology, recombinant subunit vaccines or DNA vaccines that elicit specific immunity are eminently preferable as alternatives . Recent studies have demonstrated various levels of protection by vaccination with recombinant antigen or DNA vaccines (Ding et al , 2004Kopko et al 2000;Wu et al 2004;Xu et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

Ding

Bao

et al. 2008

Parasitol Res

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Tubulins are heterodimeric molecules responsible for the polymerization of microtubules in apicomplexan parasites. The alpha-tubulin, a subcellular structural protein of Eimeria acervulina, was cloned and expressed in Escherichia coli as an alpha-tubulin-GST fusion protein. Immunogenicity of the recombinant protein was studied in chickens by subcutaneous injection of 50, 100, or 150 microg of the protein with or without Freund's adjuvant. Immunization with 150 microg alpha-tubulin-GST protein in combination with Freund's adjuvant conferred partial protection against E. acervulina oocyst challenge, as shown by a 36% reduction in oocyst shedding, a marked decrease in intestinal lesion score and a significant increase in body weight gain in comparison with the nonimmunized controls. The results suggest that alpha-tubulin protein may be used as an effective vaccine antigen for the control of Eimeria infection.

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Section: Introductionmentioning

confidence: 99%

Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

Ding

Bao

et al. 2008

Parasitol Res

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“…Cell-mediated immunity plays a key role in the protection against E. tenella in chickens, but the role of humoral immunity in E. tenella infected chickens is ambiguous (Jang et al 2010;Ding et al 2004;Wallach 2010;Abdul Hafeez et al 2006). Chickens in the subunit vaccine plus ginsomes groups had higher antibody titers than those of the subunit vaccine group before challenge.…”

Section: Discussionmentioning

confidence: 96%

“…Profilin is expressed in all sexual stages of E. tenella, stimulates cell-mediated immunity, and contains a putative conserved domain for the actin-regulatory protein profilin (Ding et al 2004;Jang et al 2010;Lee et al 2010b;Song et al 2000;Lillehoj et al 2000;Zhao et al 2011). In the present study, we investigated the adjuvant effect of ginsomes on the immune response and protection efficacy induced by recombinant profilin antigen plus ginsomes after oral infection with E. tenella sporulated oocysts.…”

Section: Discussionmentioning

confidence: 99%

“…Profilin contains an open reading frame encoding 170 residues of the amino acid from the carboxyl terminus of the E. tenella protein and expresses at Eimeria invasive stages, sporozoite and merozoite. Immunization with profilin recombinant protein or profilin DNA vaccine intranasal, intraocular, in vivo, or in ovo can induce cell-mediated immunity and protect chickens from infection of live Eimeria species Fetterer et al 2004;Song et al 2000;Min et al 2002;Ding et al 2004;Lillehoj et al 2005;Ma et al 2011;Lee et al 2010b). The induced immunity can be boosted by adding of adjuvant (Ding et al 2004;Ma et al 2011;Song and Hu 2009;Fetterer et al 2004;Min et al 2002;Song et al 2000;Lillehoj et al 2005;Lee et al 2010b), which was regarded a promising vaccine candidate (Ma et al 2011;Song et al 2009a).…”

Section: Introductionmentioning

confidence: 96%

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Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens

Zhang

Sun

et al. 2012

Parasitol Res

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An experiment was conducted to study the adjuvant effect of ginsomes on the recombinant profilin in coccidian-infected breeding birds. Three-day-old chickens were vaccinated with Eimeria tenella recombinant profilin antigen (10, 50, and 100 μg per chicken) with or without 50 μg ginsomes per chicken. The boost vaccination was carried out 14 days later. Two weeks after the booster, the chickens were challenged with 1.5 × 10(4) homologous sporulated oocysts. The specific antibody response, lymphocyte proliferation, and IL-1 release from lymphocyte were measured at 1-42 days after boost vaccination. Seven days post-challenge, the rate of survival, body weight gains (BWG) were examined then all chickens were sacrificed and lesion scores and oocysts per gram were monitored to evaluate the protective effects of the vaccination after challenge. Compared with the group of vaccinating with profilin only, groups of 50 and 100 μg antigen plus ginsomes significantly enhanced lymphocyte proliferation and IL-1 secretion. The profilin specific antibody level in the four vaccinated groups was significantly higher than in the control group and in groups vaccinated with profilin containing ginsomes than profilin only. In the groups vaccinated with profilin plus ginsomes, the BWG was significantly higher than that of group of profilin only, but there was no significant difference between profilin plus adjuvant ginsomes, diclazuril medicated and uninfected-unmedicated-unvaccinated control groups. The lesion scores in groups immunized with profilin plus ginsomes was significantly lower than that both of groups unimmunized-challenged-unmedicated control and group vaccinated with profilin only. Oocyst excretion in groups vaccinated with 50 or 100 μg profilin plus ginsomes was lower than that of groups vaccinated with profilin only. These results demonstrate that the adjuvant ginsomes can promote subunit vaccine to induce a strong immune response and protective effects.

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“…Ding et al (2004) concluded that it is possible to control this disease using maternal vaccines. Those authors claimed that the antibody transfer from mother to their progeny may be able to provide protection against clinic coccidiosis, reducing the incidence of macroscopic lesions and other clinical manifestations of this disease.…”

Section: ____________________________________________________________mentioning

confidence: 99%

Two Different Breeder Ages and Two Periods of Post-Hatching Fasting on Immunity of Broilers

Vargas¹,

Baratto²,

Bona

et al. 2009

AVS

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In this trial fertile eggs from two different flocks of breeders (aged 30 and 60 weeks) from the same commercial line were incubated under commercial conditions. After hatching, birds were feathersexed and selected. Birds were allocated in four groups. Two of these groups were fed just after placement, while the other two were fed only after 12 hours of housed. Blood samples were collected weekly, starting at day one, and antibodies against Newcastle disease, Infectious Bronchitis and Infectious Bursal Disease virus were measured. The effect of these two variables on the local reaction after a subcutaneous injection with an oil vaccine adjuvant, and the presence and severity of macroscopic lesions in the digestive system organs, at 19 and 30 days of age were also evaluated. The 12 hours fasting period had affected negatively only the Newcastle disease virus antibodies titles at 7 days of age. Both variables had no effect on the severity of macroscopic lesions in the respiratory system and in the digestive system at 19 and 30 days old.Key words: antibodies; immune response;Newcastle R RE ES SU UM MO ONeste experimento ovos férteis oriundos de matrizes de uma mesma linhagem genética, mas com idades distintas (30 e 60 semanas), foram incubados sob condições rotineiras. Após a eclosão, as aves foram sexadas, selecionadas, e alocadas em quatro grupos. Dois destes grupos receberam ração logo após o alojamento, enquanto os outros grupos foram submetidos a jejum de 12 horas. Foram realizadas colheitas de sangue no momento do alojamento e semanalmente no decorrer do experimento. Os títulos de anticorpos para as doenças de Newcastle, Gumboro e Bronquite Infecciosa foram avaliados. Também foi avaliado o efeito destas duas variáveis sobre a resposta local à aplicação subcutânea de um veículo vacinal oleoso e sobre a presença e severidade de lesões macroscópicas nos órgãos do sistema digestório aos 19 e 30 dias de idade. Somente os títulos de anticorpos contra a doença de Newcastle, medidos aos sete dias, foram afetados pelo período de jejum de 12 horas. Nem o período de jejum nem a idade de matrizes afetaram a severidade das lesões macroscópicas no sistema respiratório e digestório aos 19 e 30 dias de idade.Palavras-chave: anticorpos; Newcastle; resposta imune 164 Breeder ages and periods of post-hatching fasting on immunity of broilers ________________________________________________________________________ ________________________________________________________________________

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Protective Immunity against Eimeria acervulina following In Ovo Immunization with a Recombinant Subunit Vaccine and Cytokine Genes

Cited by 104 publications

References 49 publications

Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

Immunoprotection of chickens against Eimeria acervulina by recombinant α-tubulin protein

Adjuvant effect of ginsenoside-based nanoparticles (ginsomes) on the recombinant vaccine against Eimeria tenella in chickens

Two Different Breeder Ages and Two Periods of Post-Hatching Fasting on Immunity of Broilers

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