Protective immunity against lethal anaphylactic reaction in Toxoplasma gondii-infected mice by DNA vaccination with T. gondii-derived heat shock protein 70 gene

Kikumura, Akitoshi; Fang, Hao; Mun, Hye-Seong; Uemura, Noriko; Makino, Masayuki; Sayama, Yusuke; Norose, Kazumi; Aosai, Fumie

doi:10.1016/j.parint.2010.03.006

Cited by 18 publications

(7 citation statements)

References 33 publications

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“…Chitosan nanospheres were equally effective to FIA in increasing the survival time. These results are in agreement with other workers using temperature-sensitive mutant strain of T. gondii, ts-4 (Khan and Casciotti 1999), DNA vaccines (Yan et al 2011), recombinant T. gondii nucleoside triphosphatehydrolase-II (Tan et al 2011) recombinant surface tachyzoite antigen encapsulated in microparticles (Chuang et al 2013), recombinant Toxoplasma gondii protein disulfide isomerase (rTgPDI) and heat shock protein 70 gene (Kikumura et al 2010). On the contrary, both Spencer et al (2004) who used attenuated ts-4 strain with CPG as an adjuvant as well as Martin et al (2004) who used recombinant GRA4 or ROP2 vaccines with alum, reported no significant difference in survival time.…”

Section: Discussionsupporting

confidence: 92%

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

et al. 2014

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Toxoplasmosis, a zoonotic parasitic disease, is a huge challenge for which there is no effective vaccine up till now. In this study, chitosan nanospheres encapsulated with Toxoplasma lysate vaccine was evaluated for its ability to protect mice against both acute and chronic toxoplasmosis models of infection. Results showed that chitosan nanospheres were equally effective to Freund's incomplete adjuvant (FIA) in enhancing the efficacy of Toxoplasma lysate vaccine. The effectiveness was demonstrated by the delayed death of vaccinated mice following challenge either with virulent RH or avirulent Me49 strains, the significant decrease in parasite density in different organs, significant increase in the humoral and cellular immune response (IgG and IFN c) with a marked reduction of pathological changes in the different organs. However chitosan nanospheres were superior to FIA due to their cost effective preparation and much less necrotic changes induced in the studied organs. The success of chitosan polymer as an alternative to commonly used adjuvants paves the way for the use of other newly developed polymers to be used in the field of vaccine development.

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Section: Discussionsupporting

confidence: 92%

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

et al. 2014

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“…9B). Clinical signs were scored according to criteria reported previously (26). The average scores Ϯ SDs for the WT and IL-17A-deficient mice were 5.81 Ϯ 0.69 and 6.46 Ϯ 0.66, Ϫ B6 mice that were uninfected (uninf.…”

Section: Resultsmentioning

confidence: 99%

Interleukin-17A-Deficient Mice Are Highly Susceptible to Toxoplasma gondii Infection Due to Excessively Induced T. gondii HSP70 and Interferon Gamma Production

et al. 2017

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Interleukin17A (IL-17A) is known to be involved in the host defense against pathogens and the pathogenesis of autoimmune diseases. Previously, we showed that excessive amounts of interferon gamma (IFN-γ) play an important role in the pathogenesis of the lethal effects of by inducing anaphylactic responses. In the study described in this report, we examined the effects of IL-17A deficiency on murine host defense against oral infection. IL-17A-deficient C57BL/6 (B6) mice exhibited higher rates of mortality than wild-type (WT) mice during the acute phase of infection. CD4 T cells in the mesenteric lymph nodes (mLNs) and ileum of -infected IL-17A-deficient mice produced higher levels of IFN-γ than did those of WT mice. In addition, the level of HSP70 (HSP70) expression was also significantly increased in the ileum, mLNs, liver, and spleen of infected IL-17A-deficient mice compared with that in WT mice. These elevated levels of expression of HSP70 and IFN-γ in infected IL-17A-deficient mice were presumably linked to the IL-17A defect since they decreased to WT levels after treatment with recombinant IL-17A. Furthermore, IL-17A-deficient mice were highly susceptible to the anaphylactic effect ofHSP70, and the survival of IL-17A-deficient mice during the acute phase was improved by treatment with an anti-HSP70 monoclonal antibody. These results suggest that IL-17A plays an important role in host survival against infection by protecting the host from an anaphylactic reaction via the downregulation ofHSP70 and IFN-γ production.

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“…We have developed a DNA vaccine encoding a virulent HSP70 molecule specific for tachyzoites (i.e., the pathogenic stage of T. gondii at the acute phase or at acute exacerbation of the chronic phase of infection) targeting epidermal and dermal DC against T. gondii infection (25,31,32). The T. gondii HSP70 gene vaccine is proven to induce in vivo DC activation and successive Th1 cell polarization in the dLN, and the vaccine effects persist to the chronic phase of toxoplasmosis (31).…”

Section: Discussionmentioning

confidence: 99%

Toxoplasma gondii Infection Inhibits Th17-Mediated Spontaneous Development of Arthritis in Interleukin-1 Receptor Antagonist-Deficient Mice

et al. 2012

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ABSTRACTInterleukin 1 receptor antagonist (IL-1Ra)-deficient BALB/c mice develop spontaneous arthritis resembling human rheumatoid arthritis. We herein report that infection withToxoplasma gondii, an intracellular protozoan, is capable of ameliorating the spontaneous development of arthritis in IL-1Ra-deficient mice. The onset of arthritis development was delayed and the severity score of arthritis was significantly suppressed inT. gondii-infected mice. Expression of IL-12p40 mRNA from CD11c+cells of mesenteric lymph nodes (mLN) and spleen markedly increased at 1 week after peroral infection. While CD11c+cells also produced IL-10, IL-1β, and IL-6, CD4+T cells fromT. gondii-infected mice expressed significantly high levels of T-bet and gamma interferon (IFN-γ) mRNA in both mLN and spleen. Levels of GATA-3/IL-4 mRNA or RORγt/IL-17 mRNA decreased in the infected mice, indicating Th1 cell polarization and the reduction of Th2 and Th17 cell polarization. The severity of arthritis was related to Th1 cell polarization accompanied by Th17 cell reduction, demonstrating the protective role of theT. gondii-derived Th1 response against Th17 cell-mediated arthritis in IL-1Ra-deficient mice.

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Protective immunity against lethal anaphylactic reaction in Toxoplasma gondii-infected mice by DNA vaccination with T. gondii-derived heat shock protein 70 gene

Cited by 18 publications

References 33 publications

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

The effect of chitosan nanospheres on the immunogenicity of Toxoplasma lysate vaccine in mice

Interleukin-17A-Deficient Mice Are Highly Susceptible to Toxoplasma gondii Infection Due to Excessively Induced T. gondii HSP70 and Interferon Gamma Production

Toxoplasma gondii Infection Inhibits Th17-Mediated Spontaneous Development of Arthritis in Interleukin-1 Receptor Antagonist-Deficient Mice

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