Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus

Mohamed, Walid; Sethi, Shneh; Tchatalbachev, Svetlin; Darji, Ayub; Chakraborty, Trinad

doi:10.1371/journal.pone.0035503

Cited by 14 publications

(14 citation statements)

References 42 publications

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“…In the case of listeriosis vaccination, the emphasis has been on recombinant vaccines using live attenuated pathogens, metabolically active but non-viable bacteria or non-pathogenic bacteria harboring virulence gene clusters, although some of them do not trigger strong innate immune responses and require adjuvants (Brockstedt et al, 2005; Bruhn et al, 2007; Mohamed et al, 2012). These recombinant vaccines might also present safety concerns because live vaccines if administered to immunocompromised individuals might cause cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Cellular vaccines in listeriosis: role of the Listeria antigen GAPDH

Calderon-Gonzalez

Frande-Cabanes

Bronchalo-Vicente

et al. 2014

Front. Cell. Infect. Microbiol.

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The use of live Listeria-based vaccines carries serious difficulties when administrated to immunocompromised individuals. However, cellular carriers have the advantage of inducing multivalent innate immunity as well as cell-mediated immune responses, constituting novel and secure vaccine strategies in listeriosis. Here, we compare the protective efficacy of dendritic cells (DCs) and macrophages and their safety. We examined the immune response of these vaccine vectors using two Listeria antigens, listeriolysin O (LLO) and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), and several epitopes such as the LLO peptides, LLO189−201 and LLO91−99 and the GAPDH peptide, GAPDH1−22. We discarded macrophages as safe vaccine vectors because they show anti-Listeria protection but also high cytotoxicity. DCs loaded with GAPDH1−22 peptide conferred higher protection and security against listeriosis than the widely explored LLO91−99 peptide. Anti-Listeria protection was related to the changes in DC maturation caused by these epitopes, with high production of interleukin-12 as well as significant levels of other Th1 cytokines such as monocyte chemotactic protein-1, tumor necrosis factor-α, and interferon-γ, and with the induction of GAPDH1−22-specific CD4+ and CD8+ immune responses. This is believed to be the first study to explore the use of a novel GAPDH antigen as a potential DC-based vaccine candidate for listeriosis, whose efficiency appears to highlight the relevance of vaccine designs containing multiple CD4+ and CD8+ epitopes.

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Section: Discussionmentioning

confidence: 99%

Cellular vaccines in listeriosis: role of the Listeria antigen GAPDH

Calderon-Gonzalez

Frande-Cabanes

Bronchalo-Vicente

et al. 2014

Front. Cell. Infect. Microbiol.

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“…For development of protective immunity against L. monocytogenes, recent advances in the field of biotechnology has greatly aided (Mohamed et al 2012). As the bacterium is found intracellular, it induces a long-lasting cellular immune response and as a consequence an attenuated vaccine may be a good candidate for vaccination.…”

Section: Vaccinesmentioning

confidence: 99%

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Dhama

Karthik

Tiwari

et al. 2015

Veterinary Quarterly

135

108

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Listeriosis is an infectious and fatal disease of animals, birds, fish, crustaceans and humans. It is an important food-borne zoonosis caused by Listeria monocytogenes, an intracellular pathogen with unique potential to spread from cell to cell, thereby crossing blood-brain, intestinal and placental barriers. The organism possesses a pile of virulence factors that help to infect the host and evade from host immune machinery. Though disease occurrence is sporadic throughout the world, it can result in severe damage during an outbreak. Listeriosis is characterized by septicaemia, encephalitis, meningitis, meningoencephalitis, abortion, stillbirth, perinatal infections and gastroenteritis with the incubation period varying with the form of infection. L. monocytogenes has been isolated worldwide from humans, animals, poultry, environmental sources like soil, river, decaying plants, and food sources like milk, meat and their products, seafood and vegetables. Since appropriate vaccines are not available and infection is mainly transmitted through foods in humans and animals, hygienic practices can prevent its spread. The present review describes etiology, epidemiology, transmission, clinical signs, post-mortem lesions, pathogenesis, public health significance, and advances in diagnosis, vaccines and treatment of this disease. Special attention has been given to novel as well as prospective emerging therapies that include bacteriophage and cytokine therapy, avian egg yolk antibodies and herbal therapy. Various vaccines, including advances in recombinant and DNA vaccines and their modes of eliciting immune response, are also discussed. Due focus has also been given regarding appropriate prevention and control strategies to be adapted for better management of this zoonotic disease.

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“…While there is no doubt that P. salmonis is an intracellular pathogen (27), it has not been fully documented whether the bacterium resides in endosomes/phagosomes [such as Mycobacterium sp. (51)] or is released from the initial endosome/phagosome to the cytosol, as seen for L. monocytogenes (52, 53). As was mentioned earlier, there are studies indicating that P. salmonis induces formation of vesicular structures from actin disorganization and de novo synthesis (18), but it is not known if this is also the site of bacterial growth/division and a source of spread to neighboring cells.…”

Section: Immune Responses Induced By Dna and Replicating Vaccinesmentioning

confidence: 99%

“…The subcellular localization of the bacterium will impact on what type of immune responses would be required for induction of protective immunity. Translating the knowledge from L. monocytogenes vaccination studies, it is reasonable to assume that cell-mediated immunity, particularly cytotoxic immune responses, is needed (53) in addition to strong antibody responses.…”

Section: Immune Responses Induced By Dna and Replicating Vaccinesmentioning

confidence: 99%

Immunization Strategies against Piscirickettsia salmonis Infections: Review of Vaccination Approaches and Modalities and Their Associated Immune Response Profiles

Evensen

2016

Front. Immunol.

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Salmonid rickettsial septicemia (SRS) is a serious, infectious disease in Chilean salmon farming caused by Piscirickettsia salmonis, causing heavy losses to the salmonid industry. P. salmonis belongs to the Gammaproteobacteria, order Thiotrichales. SRS was first described in Chile in 1989, and infection with P. salmonis has since been described from a high number of fish species and in several geographic regions globally. P. salmonis infection of salmonids causes multifocal, necrotic areas of internal organs such as liver, kidney, and spleen. Histologically and immunologically, the tissue response is the formation of granulomas, often with central suppuration. The exact sequence of infection is not known, but bacteria likely gain access to internal organs through mucosal surfaces and when infected, fish carry bacteria in macrophages. It has not been fully determined if the bacterium resides in the cytosol or “hide” within vesicular structures intracellularly, although there are indications that in vitro infection results in actin reorganization and formation of actin-coated vesicle within which the bacterium resides. Protection against lethal challenge is well documented in lab scale experiments, but protection from vaccination has proven more difficult to attain long term under field conditions. Current vaccination protocols include whole cell, inactivated and adjuvanted vaccines for injection for primary immunization followed by oral boost where timing of boost delivery is followed by measuring circulating antibody levels against the pathogen. Documentation also exist that there is correlation between antibody titers and protection against mortality. Future vaccination regimes will likely also include live-attenuated vaccines or other technologies such as DNA vaccination. So far, there is no documentation available for live vaccines and, for DNA vaccines, studies have been unsuccessful under laboratory conditions.

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Protective Immunity to Listeria Monocytogenes Infection Mediated by Recombinant Listeria innocua Harboring the VGC Locus

Cited by 14 publications

References 42 publications

Cellular vaccines in listeriosis: role of the Listeria antigen GAPDH

Cellular vaccines in listeriosis: role of the Listeria antigen GAPDH

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

Immunization Strategies against Piscirickettsia salmonis Infections: Review of Vaccination Approaches and Modalities and Their Associated Immune Response Profiles

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