Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

Saravia, Nancy Gore; Hazbón, Manzour Hernando; Osorio, Yaneth; Valderrama, Liliana; Walker, Susan; Santrich, Cecilia; Cortázar, Tania M.; LeBowitz, Jonathan H.; Travi, Bruno L.

doi:10.1016/j.vaccine.2004.07.044

Cited by 29 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, there was a slight and transient exacerbation of the lesions in males immunized with the empty plasmid vector between weeks 4-11 weeks of infection, but this did not reach statistical significance. tion is in agreement with a previous report (Saravia et al, 2005). Male DBA/2 mice have also been reported to be more susceptible to L. mexicana infection (Satoskar & Alexander, 1995) and male hamsters to L. panamensis and L. guyanensis (Travi et al, 2002).…”

supporting

confidence: 93%

“…Disease development was monitored for up to 17 weeks after infection by weekly measurement of footpad size with a vernier caliper, and lesion size was expressed as the difference in size between the infected and the contralateral uninfected footpad as described before (Saravia et al, 2005). Footpad swelling is indeed well correlated with parasite load during murine infection with L. mexicana (Aguilar Torrentera et al, 2002), as well as in golden hamsters infected with L. brasiliensis or L. amazonensis (Sinagra et al, 2007).…”

Section: Mexicana Infectionmentioning

confidence: 99%

See 1 more Smart Citation

A combination DNA vaccine encoding nucleoside hydrolase 36 and glycoproteine 63 protects female but not male hamsters againstLeishmania mexicana

et al. 2009

View full text Add to dashboard Cite

Summary :Leishmaniasis is a group of diseases caused by protozoan parasites of the Leishmania genus. Previous studies have shown that a DNA vaccine encoding Leishmania donovani antigen nucleoside hydrolase 36 and L. mexicana glycoprotein 63 is protective in mice. We investigated here the efficacy of this DNA vaccine to induce protection in golden hamsters. Male hamsters were more susceptible to infection by Leishmania mexicana than females. Following immunization with two doses of the DNA vaccine, only females resulted protected while males developed normal lesions. Résumé

show abstract

supporting

confidence: 93%

Section: Mexicana Infectionmentioning

confidence: 99%

A combination DNA vaccine encoding nucleoside hydrolase 36 and glycoproteine 63 protects female but not male hamsters againstLeishmania mexicana

et al. 2009

View full text Add to dashboard Cite

show abstract

“…More over, it is interesting to note that they bear no homology to any human and livestock animal proteins . PFR proteins have been demonstrated to be immunogenic when PFR2 was used alone (Saravia et al 2004) and/or co-administered together with PFR1 and PFR2 against T. cruzi infection in mice . Immunisation with purified PFR proteins from T. cruzi epimastigote was shown to completely protect the mice from a subsequent challenge with fatal dose of this parasite (Wrightsman et al 1995).…”

Section: Role As a Vaccine Targetmentioning

confidence: 99%

“…Since antibodies from human patients suffering from Chagas's disease also recognised PFR proteins (Wrightsman et al 1995;Michailowsky et al 2003). Saravia et al (2005) identified PFR2 as a potential vaccine target against Leishmania infection. Wrightsman and Manning (2000) reported that PFR antigen co-absorbed onto alum with rIL-12 or adenovirus expressed IL-12 could elicit a cell mediated immune response of Th1 type that provides protection against lethal challenge when subcutaneously delivered.…”

Section: Role As a Vaccine Targetmentioning

confidence: 99%

“…To answer the pertinent question of immune recognition of PFR, since it is a hidden antigen, it has been theorised that the degradation of flagellum and PFR during the transition from promastigote to amastigote form, the components are made available for immune recognition (Michailowsky et al 2003;Saravia et al 2005). Another possibility of exposure of the hidden antigens to the host immune system exists following death and lysis of the parasite.…”

Section: Role As a Vaccine Targetmentioning

confidence: 99%

See 1 more Smart Citation

An overview on kinetoplastid paraflagellar rod

2014

View full text Add to dashboard Cite

Kinetoplastids, the evolutionary ancient organisms exhibit a rich and diverse biology which epitomizes many of the fascinating topics of recent interest and study. These organisms possess a multifunctional organelle, the flagellum containing a canonical 9 ? 2 axoneme which is involved in vital roles, viz. parasite cell division, morphogenesis, motility and immune evasion. Since Antony Van Leeuwenhoek's innovative explanation of 'little legs' helping the movements of microbes in 1975, this biological nanomachine has captured the thoughts of scientists. The core structure of kinetoplastid flagellum is embroidered with a range of extra-axonemal structures such as paraflagellar rod (PFR), a large lattice like structure which extends alongside the axoneme from the flagellar pocket to the flagellar tip. The coding sequences for significant components of PFR are highly conserved throughout the Kinetoplastida and Euglenida. The high order organization and restricted evolutionary distribution of the PFR components and structure makes the PFR a particularly valuable therapeutic and prophylactic target. This review focuses on the recent developments in identification of ultra structural components of PFR in order to understand the function of this intriguing organelle and devising strategies for therapeutic interventions.

show abstract

The Emergence of Defined Subunit Vaccines for the Prevention of Leishmaniasis

Duthie

Reed

2014

Curr Trop Med Rep

View full text Add to dashboard Cite

Infection with Leishmania parasites can cause disease across a range of clinical manifestations and outcomes. Given the practical difficulties in maintaining vector control programs, vaccination is likely the only way in which the sustained reduction or elimination of the leishmaniases can be achieved. In this review, we focus on the development of second-generation vaccines with emphasis on defined subunit vaccines using recombinant expression techniques. We highlight particular antigens that have provided protection in animal models, and discuss methods, such as inclusion in chimeric fusion proteins and use of appropriate adjuvants, that are likely required to transition these into clinically-relevant candidates. We also outline the alternative strategies, such as attenuated parasites, DNA vaccination, and use of sand fly proteins, that are being proposed.

show abstract

Protective immunogenicity of the paraflagellar rod protein 2 of Leishmania mexicana

Cited by 29 publications

References 42 publications

A combination DNA vaccine encoding nucleoside hydrolase 36 and glycoproteine 63 protects female but not male hamsters againstLeishmania mexicana

A combination DNA vaccine encoding nucleoside hydrolase 36 and glycoproteine 63 protects female but not male hamsters againstLeishmania mexicana

An overview on kinetoplastid paraflagellar rod

The Emergence of Defined Subunit Vaccines for the Prevention of Leishmaniasis

Contact Info

Product

Resources

About