Protective influence of Carica papaya L. aqueous leaf extract against hyperuricemia and acute renal injury in a murine model

Abstract

Abstract. This study was conducted as a pilot investigation on the protective influence of Carica papaya L. aqueous leaf extract (PLE) against experimental hyperuricemia and acute renal injury in an animal model. The effects of oral pre-treatment with PLE on BUA (blood uric acid) levels and renal histopathology of potassium bromate (KBrO 3 )-treated adult male albino mice were assessed. Sixty mice were randomized to six groups (n = 10): sham (sterile water) and KBrO 3 controls; three experimental groups: PLE1, PLE2 and PLE3 (1, 2 and 3 g/kg body weight [BW] + KBrO 3 ); and ascorbic acid (ascorbic acid 200 mg/kg BW + KBrO 3 ) comparator. Oral pre-treatment with PLE was given for 14 d via gastric gavage. On day 14, except on the sham control, hyperuricemia was induced by oral administration of a single dose of KBrO 3 200 mg/kg body weight. BUA levels were measured pre-and post-KBrO3 induction (at 333 h and 336 h, respectively). The kidneys were then immediately excised under anesthesia. Histopathologic evaluation of the kidneys was done using a standard numerical grading scheme. Results showed a significant increase in BUA levels in the KBrO 3 control (p < 0.001). In the PLE groups, BUA levels were not significantly increased (p > 0.05). Histologically, there was significant attenuation of acute renal tissue damage in the PLE groups. However, the BUA and histopathologic responses of the PLE groups were not dose-dependent (p = 0.80 and p = 0.66, respectively). These preliminary findings suggest that PLE may have antihyperuricemic and nephroprotective effects in this murine model of hyperuricemia and acute renal tissue injury.

Potensi Tanaman Pepaya (Carica papaya L.) sebagai Nefroprotektor

“…Selain itu, pemeriksaan histopatologi pada kelompok tikus yang diberi ekstrak air daun pepaya menunjukkan adanya kerusakan sedang pada jaringan, lalu untuk kelompok tikus yang diberi potasium bromat saja mengalami kerusakan ekstensif pada jaringan ginjal. Efek nefroprotektif ekstrak air daun pepaya tersebut diduga berasal dari kandungan alkaloid, flavonoid, dan tanin [45]. Hal tersebut sejalan dengan penelitian Francis et al (2020) yang menguji efektivitas ekstrak etanol daun pepaya pada tikus wistar yang diinduksi merkuri klorida.…”