Protective role of carnitine in breast cancer via decreasing arginase activity and increasing nitric oxide

Erbas, Hakan; Aydoğdu, Nurettin; Usta, Ufuk; Erten, Oya

doi:10.1016/j.cellbi.2007.06.007

Cited by 20 publications

(19 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with this finding, Erbas et al (2007) reported that L-carnitine increased NO through reducing the activity of arginase and elevating that of NO synthase. Another study reported by Sharifi et al (2009) indicated that L-carnitine reduced the activity of angiotensin-converting enzyme in the aorta, heart and kidney in rats, which resulted in higher NO production.…”

Section: Discussionsupporting

confidence: 67%

“…Besides, L-carnitine participates in scavenging reactive oxygen species (Liu et al, 2004) and immunomodulation (Buyse et al, 2007). Erbas et al (2007) indicated that L-carnitine could increase plasma nitric oxide (NO) in human subjects. Nitric oxide is a potent vasodilator that opposes the onset of PHS in broiler chickens subjected to hypoxia (Khajali and Wideman, 2010).…”

mentioning

confidence: 99%

See 1 more Smart Citation

L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude

Khajali

2014

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

An experiment was carried out to examine the effects of L-carnitine supplementation on growth performance and cardiopulmonary function of broiler chickens reared at high altitude (2100 m above sea level). A total of 96 day-old male chicks (Cobb 500) were randomly assigned into two dietary treatments containing 0 (control group) and 200 mg/kg L-carnitine. The experimental diets were fed for a period of 42 days consisting of the starting (days 1 to 21) and growing periods (days 21 to 42). Nutrient requirements of chickens met the NRC (1994) recommendations. The results showed that dietary L-carnitine had no significant influence on body weight gain, feed intake and feed conversion ratio. L-carnitine reduced plasma concentration of malondialdehyde, packed cell volume (PCV) and abdominal fat deposition compared to the control (P < 0.05). A significant (P < 0.05) decrease was observed in the right to total ventricular weight ratio (RV:TV) in birds receiving L-carnitine when compared to the control. Supplementation of L-carnitine increased plasma nitric oxide and immune responsiveness, which manifested in an increased toe-web thickness index measured at 24 h following the injection of phytohaemagglutinin P. In conclusion, supplementation of Lcarnitine had beneficial effects on preventing lipid peroxidation and pulmonary hypertension in broiler chickens raised at high altitude.

show abstract

Section: Discussionsupporting

confidence: 67%

mentioning

confidence: 99%

L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude

Khajali

2014

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

show abstract

“…L-carnitine enhances myocardial function by improving fatty acid transport into the mitochondria (Ueland et al, 2012). Recently, Erbas et al (2007) indicated that L-carnitine could increase plasma nitric oxide (NO) in human subjects. Nitric oxide is a potent vasodilator that opposes the onset of PHS in broiler chickens (Khajali and Wideman, 2010).…”

Section: Introductionmentioning

confidence: 99%

Dietary L-carnitine Improves Pulmonary Hypertensive Response in Broiler Chickens Subjected to Hypobaric Hypoxia

et al. 2013

View full text Add to dashboard Cite

The present study was conducted to examine the effects of L-carnitine on pulmonary hypertensive response in broiler chickens reared at high altitude and exposed to hypobaric hypoxia. A total of 192 day-old male broilers (Cobb 500) were randomly assigned to 4 treatments and 4 replicates of 12 chicks. A basal diet composed of mainly corn and soybean meal was formulated and served as a control. Three additional treatments were made by supplementing graded levels of L-carnitine (50, 100, and 150 mg/kg). Chicks received dietary treatments at free access from 1 to 42 days of age. Results indicated that feeding L-carnitine at 100 mg/kg caused a significant increase in plasma nitric oxide (NO) with concomitant decrease in plasma malonedialdehyde (MDA). The Lead II electrocardiogram indicated reductions of S wave amplitude for all three doses of L-carnitine relative to the control and the difference between the birds that received L-carnitine at 50 mg/kg and the control was significant (P＜0.05). The right ventricular weight ratio (RV/TV) tended to decline when L-carnitine supplemented to diets. In conclusion, L-carnitine reduced ascites mortality in broiler chickens by increased NO production, reduced MDA concentration, and reduced right ventricular hypertrophy.

show abstract

“…However, at physiological pH, the Vmax of each of the NOS enzymes was less than 1000-times that of arginase (21); therefore, it was concluded that both NOS and arginase could use arginine (22). Previously, we also showed that there was a negative relation between arginase enzyme activities and NO levels in breast cancer (15). Nitric oxide is a pervasive molecule that employs many biological outcomes (5).…”

Section: Serum Tissuementioning

confidence: 99%

“…Breast cancer tissue arginase activity was measured spectrophotometrically according to the tiosemicarbazide diacetylmonoxime urea (TDMU) method (15).…”

Section: Determination Of Argininase Enzyme Activity In Serummentioning

confidence: 99%

Effect of Rosuvastatin on Arginase Enzyme Activity and Polyamine Production in Experimental Breast Cancer

Erbas¹,

Bal²,

Çakır³

2015

Balkan Med J

Self Cite

View full text Add to dashboard Cite

Breast cancer is still the commonest type of cancer in women. It accounts for about 20% of all cancer-related mortality. Therefore, great medical and scientific efforts are continually invested into understanding the disease's pathology and finding new methods for its early diagnosis, prevention and treatment.In cells, L-arginine is embraced in protein synthesis. Arginine is also used by arginase, arginine decarboxylase, nitric oxide synthases (NOSs) and glycine transaminase. Arginase is a crucial enzyme that is chargeable for nitrogen metabolism. Arginine is its main substrate; from this, urea and L-ornithine are formed (1). There are two types of arginase: Arginase I is cytosolic and mostly found in the liver; it is considered to be primarily liable for the detoxification of ammonia. The other isoenzyme, arginase II, is engaged in ornithine creation. Ornithine is the precursor of some products which take place in cell growth: glutamate, proline and polyamines (spermine, Background: Breast cancer is the most common malignant tumour of women around the world. As a key enzyme of the urea cycle, arginase leads to the formation of urea and ornithine from L-arginine. In the patients with several different cancers, arginase has been found to be higher and reported to be a useful biological marker. Aims: The aim of this study was to investigate the effect of rosuvastatin on serum and cancer tissue arginase enzyme activity, and ornithine and polyamine (putrescine, spermidine, spermine) levels. Study Design: Animal experiment. Methods: In this study, 50 male Balb/c mice were used. Erchlich acid tumour cells were injected into the subcutaneous part of their left foot. The mice were divided into five groups: healthy control group, healthy treatment, tumour control, treatment 1 and treatment 2. Then, 1 mg/kg and 20 mg/kg doses of rosuvastatin were given intraperitoneally. Serum and tissue arginase enzyme activities and tissue ornithine levels were determined spectrophotometrically. HPLC measurement of polyamines were applied. Results: Increased serum arginase activity and polyamine levels were significantly decreased with rosuvastatin treatment. In the tumour tissue, arginase activity and ornithine levels were significantly decreased in treatment groups compared to the tumour group. Tissue polyamine levels also decreased with rosuvastatin treatment. Conclusion:We suggest that rosuvastatin may have some protective effects on breast cancer development as it inhibits arginase enzyme activity and ornithine levels, precursors of polyamines, and also polyamine levels. This protective effect may be through the induction of nitric oxide (NO) production via nitric oxide synthase (NOS). As a promising anticancer agent, the net effects of rosuvastatin in this mechanism should be supported with more advanced studies and new parameters. Keywords: Arginase, breast cancer, ornithine, polyamines, rosuvastatin Copyright 2015 © Trakya University Faculty of Medicine Balkan Med J 201532:89-95

show abstract

Protective role of carnitine in breast cancer via decreasing arginase activity and increasing nitric oxide

Cited by 20 publications

References 40 publications

L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude

L-carnitine supplementation decreases lipid peroxidation and improves cardiopulmonary function in broiler chickens reared at high altitude

Dietary L-carnitine Improves Pulmonary Hypertensive Response in Broiler Chickens Subjected to Hypobaric Hypoxia

Effect of Rosuvastatin on Arginase Enzyme Activity and Polyamine Production in Experimental Breast Cancer

Contact Info

Product

Resources

About