Protective role of melatonin supplementation against nicotine-induced liver damage in mouse

Abstract

The present study was carried out to determine histopathological effects of nicotine, one of the most significant components of tobacco, on mouse liver and ameliorative effect of melatonin on liver damage. A total of 140 mature Swiss Albino mice (Mus musculus) were divided into four experimental groups: control group, nicotine group, melatonin group and nicotine + melatonin group. Each group was further subdivided into seven groups (five mice each) according to the time of killing (12 h and days 1, 3, 5, 7, 14 and 21 after drug administration). In nicotine and nicotine + melatonin groups, 3 mg/kg of nicotine was injected intraperitoneally every day until killing. The nicotine + melatonin group was additionally injected with 10 mg/kg of melatonin after 30 min of nicotine injection. The melatonin group was injected only with 10 mg/kg of melatonin every day until killing. All the treatments were given 2 h before sunset, when melatonin receptors were active. After the last injection, five mice from each group were killed at 12th hour and on days 1, 3, 5, 7, 14 and 21; the livers were removed for histopathological processing by light microscopy. The histopathological results revealed time-dependent degeneration in the livers of mice in nicotine group. Regenerative changes in the nicotine and melatonin groups were observed when compared with nicotine groups.

Efficacy of Melatonin against Oxidative Stress, DNA Damage and Histopathological Changes Induced by Nicotine in Liver and Kidneys of Male Rats

“…Concerning the effect of melatonin on the nicotine induced liver histopathological changes, the obtained result indicated that melatonin at a dose of 10mg completely counteracted the nicotine induced hepatic damage and retuned it back to normal as in the control group. These results are matching with those of Mercan and Eren (2012) who found that melatonin at 10mg could protect liver of nicotine treated mice from histopathological changes and this may be due to its antioxidants effect, moreover, Moradpour et al (2020) in his recent study recorded that melatonin improved liver damage and histopathological changes caused by mobile phone radiation in mice model.…”

“…The result of the current study revealed that nicotine rats showed degeneration and necrosis of the hepatic cells and dilatation of the hepatic sinusoids. Adedayo et al (2011) reported that administration of male rats with nicotine induced vacuolation in the liver parenchyma, necrosis of hepatocytes and degeneration of the cells lining the bile ducts, also Mercan and Eren (2012) found that administration of mice with nicotine induced mononuclear cell infiltration, sinusoidal dilatation and hydropic degeneration. These histopathological changes may be due to increasing metabolic activity of cells for removal of toxins from the body during the toxification process (Milad and Zahra, 2019).…”

“…All treatments were given 2 hrs. before sunset, when melatonin receptors were active (Mercan and Eren 2012).…”

Melatonin attenuates thiocyanate-induced vasoconstriction in aortic rings

“…The same group also reported about the reduction of vasoconstriction and improvement of endothelial physiology by melatonin (Rodella et al, 2010b). In a recent study, the protective role of melatonin supplementation against nicotine-induced liver damage in mice has been reported (Mercan and Eren, 2013). The presented study was thus undertaken to quantify the thiocyanate-induced vasoconstriction in rat aortic segments and further to examine the potential of melatonin in attenuating the generated vasomotor response.,…”