Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks

Saleh, Sohair Y.; Sawiress, F. A. R.; Tony, Merinrose; Hassanin, A. M.; Khattab, M.; Bakeer, Manal Ramadan

doi:10.5539/jas.v7n10p239

Cited by 8 publications

(6 citation statements)

References 45 publications

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“…In line with our previous results, the experiments showed that the administration of MK-801 to rat testis resulted in increased oxidative stress in rat testis [43]. Similarly, Saleh et al demonstrated that the administration of MK-801 to rabbits increased the atrophy of the tubular structure and the degeneration of germinal cells in the lumen of the seminiferous tubule in histopathological evaluation [44]. Moreover, it was reported that the level of total antioxidant status and sperm kinematics parameters have decreased following the administration of MK-801 [45,46].…”

Section: Discussionsupporting

confidence: 87%

Effect of NMDA Receptor Agonist and Antagonist on Spermatogonia Stem Cells Proliferation in 2- and 3- Dimensional Culture Systems

Noghani

Asadpour

Saberivand

et al. 2021

Preprint

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The main purpose of this study was to investigate the effect of D-serine (DS) and Dizocilpine (MK-801or Mk) on the proliferation of SSCs in two-dimensional (2D) and three-dimensional (3D) culture systems. The SSCs of male NMRI mice were isolated by enzymatic digestion and cultured for two weeks. Then, the identity of SSCs was confirmed by anti-Plzf and anti-GFR-α1 antibodies via immunocytochemistry (ICC). The proliferation capacity of SSCs was evaluated by their culture on a layer of the decellularized testicular matrix (DTM) prepared from mouse testis, as well as two-dimensional (2D) with different mediums. After two weeks of the initiation of proliferation culture on 3D and 2D medium, the pre-meiotic at the mRNA and protein levels were evaluated via qRT-PCR and flow cytometry methods, respectively. The results showed that the proliferation rate of SSCs in three-dimensional culture with 50 mM glutamic acid and 20 mM D-serine was significantly different from other groups after 14 days treatment. mRNA expression levels of Plzf in 3D-cultures supplemented by 20 mM D-serine and 50 mM glutamic acid were considerably higher than the 3D control group (p<0.001). The flow cytometry analysis revealed that the amount of Plzf in the 2D-culture groups of SSCs with 20mM MK-801 was considerably lower compared to the 2D-culture control group (p<0.001). This study indicated that decellularized testicular matrix supplemented with D-serine and glutamic acid could be considered a promising vehicle to support cells and provide an appropriate niche for the proliferation of SSCs.

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Section: Discussionsupporting

confidence: 87%

Effect of NMDA Receptor Agonist and Antagonist on Spermatogonia Stem Cells Proliferation in 2- and 3- Dimensional Culture Systems

Noghani

Asadpour

Saberivand

et al. 2021

Preprint

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“…In another study on the rabbit bucks, MK-801 injection was caused a significant decrease in sperm number, antioxidant parameters and pathological changes in testis. Dietary supplementation of vitamin E, vitamin C, and olive pomace ameliorated the related parameters in the testis (19). In the present study, the emerged oxidative stress sourced by the MK-801 decreased the testicular weight same as previous studies (14,16).…”

Section: Discussionsupporting

confidence: 86%

Protective effects of resveratrol on testicular oxidative stress induced of MK-801 in mice

Akosman¹,

Türkmen²,

Demirel³

et al. 2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Recently, it has been discovered that the doses of the MK-801 causing schizophrenia also initiate the oxidative stress in the testis. The current study investigated the protective role of the resveratrol against the MK-801 induced oxidative stress in the testis in mice. The testis weight, the total oxidant-antioxidant status, seminiferous tubules diameter, epithelial height, testicular pathology, and epididymal sperm motility were evaluated. A total of 24 male mice were equally divided into 4 groups so that each group included 6 mice. In the study, Group I (control group) was intraperitoneally received with 0.9% saline (10ml/kg). Group II was i.p. administered with MK-801 (1mg/kg), Group III was treated with i.p. MK-801 (1mg/kg) and resveratrol (40mg/kg), and the Group IV was treated i.p. with resveratrol (40mg/kg). All injections were performed for 14 days. According to the results, in the testis of mice in Group II the oxidative stress was observed. The oxidative stress affected the seminiferous tubules pathologically and decreased the weight of the testis and sperm motility. However, resveratrol protected the MK-801 induced oxidative stress in the testis. Moreover, this dose of the resveratrol increased the sperm motility compared with the controls. In conclusion, MK-801 caused oxidative stress in the testis and resveratrol had a protective effect against this damage.

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“…The control group (C) was fed the control diet. The other three groups of rabbits comprised the treatment groups: group P, supplemented with 4.5% pomegranate peel in their diet (Zeweil, & Elgindy, 2016); group O, supplemented with 10% olive pomace in their diet (Saleh et al, 2015); and group PO, supplemented with a mixture of pomegranate peel and olive pomace. After the end of the one‐month adaption period, the does were randomly selected and used for the experiments.…”

Section: Methodsmentioning

confidence: 99%

Effects of pomegranate peel and olive pomace supplementation on reproduction and oxidative status of rabbit doe

Bakeer

Abdelrahman

Khalil

2021

Animal Physiology Nutrition

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The current study aimed to investigate the effects of pomegranate peel and olive pomace supplementation on the reproductive hormones, antioxidative status, reproductive capacity and maternal behaviour of rabbit does. Forty does were used for the experiments. The animals were randomly assigned to four groups of ten does.One group was fed on the control diet and was considered the control group (C). The second group was supplemented with 4.5% pomegranate peel in their diet (P), the third group was supplemented with 10% olive pomace in their diet (O), and the fourth group was supplemented with a mixture of pomegranate peel and olive pomace (PO).Compared with the control does, group P showed significantly increased serum levels of gonadotropic hormones and oestradiol-17β two hours after mating, on the 20th day of lactation and after weaning; significantly increased progesterone levels at midpregnancy; and significantly increased in prolactin levels on the 10th day of lactation.Additionally, the results revealed significant increases in total DNA, protein concentration, litter size, milk yield and nest traits of groups P and PO. In conclusion, pomegranate peel supplementation improves the reproductive performance of does and increases their antioxidant parameters.

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Protective Role of Some Feed Additives against Dizocelpine Induced Oxidative Stress in Testes of Rabbit Bucks

Cited by 8 publications

References 45 publications

Effect of NMDA Receptor Agonist and Antagonist on Spermatogonia Stem Cells Proliferation in 2- and 3- Dimensional Culture Systems

Effect of NMDA Receptor Agonist and Antagonist on Spermatogonia Stem Cells Proliferation in 2- and 3- Dimensional Culture Systems

Protective effects of resveratrol on testicular oxidative stress induced of MK-801 in mice

Effects of pomegranate peel and olive pomace supplementation on reproduction and oxidative status of rabbit doe

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