Protein-activated transformation of silver nanoparticles into blue and red-emitting nanoclusters

Sahu, Dillip Kumar; Sarkar, Priyanka; Singha, Debabrata; Sahu, Kalyanasis

doi:10.1039/c9ra06774d

Cited by 17 publications

(10 citation statements)

References 46 publications

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“…Over the years, atomically precise noble metal nanoclusters (MNCs) have received immense interest due to widespread opportunities in sensing, − multimodal imaging, , targeted drug delivery, − therapeutic applications, , biolabeling, , and so on. , These MNCs feature unique molecule-like fluorescence (FL) properties due to the presence of discrete energy levels, unlike their larger plasmonic nanoparticle counterparts . Contrary to most extensively studied gold nanoclusters (AuNCs), silver nanoclusters (AgNCs) remain less explored due to their propensity to aggregate in an aqueous phase because of their higher surface energy, making the synthesis of AgNCs a bit challenging. , Particularly, proteins as stabilizers have come out as a boon to researchers presenting a bio-friendly avenue to produce water-soluble and stable AgNCs. − However, toxic reducing agents such as NaBH 4 are often used to synthesize most red-emitting protein-stabilized AgNCs, which may limit their application in a biological system. , Zhou et al synthesized red-emitting AgNCs using NaBH 4 as a reducing agent and lysozyme (LYS) as a stabilizer . However, the quantum yield (QY) was only 1.3%.…”

Section: Introductionmentioning

confidence: 99%

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

Sarkar

Saha

Nandi

et al. 2022

ACS Appl. Nano Mater.

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Biomolecule-stabilized metal nanoclusters (MNCs) exhibit enormous potential as unique luminescent materials in various applications. However, synthesis of highly stable and bio-friendly fluorescent MNCs is still a challenge. Here, we report a facile synthesis of red-emitting silver nanoclusters (LYS–AgNCs) within a dithiothreitol-reduced lysozyme (LYS) scaffold. The nanoclusters exhibit a uniform size distribution, excellent water solubility, and superior photoluminescence properties featuring a quantum yield of 6.1%, a massive (280 nm) Stokes shift, and solid-state emission, and pH stability augments their applicability in dual-mode sensing platforms for Cu2+ and vitamin B12 (VB12). Two contrasting fluorescence (FL)-quenching mechanisms are responsible for the sensing; Cu2+-induced FL quenching occurs via a multifaceted mechanism involving both static and dynamic quenching, whereas the inner filter effect and Förster resonance energy transfer are mainly responsible for VB12-induced FL quenching. An inexpensive portable paper strip is fabricated using LYS–AgNCs for on-site field application, enabling instrument-free fast and visual detection of Cu2+ and VB12. Moreover, LYS–AgNCs also possess favorable biocompatibility against human cervical cancer cells (HeLa), making them a suitable nanoprobe for cell imaging and an efficient agent for detecting Cu2+ and VB12 inside live cells as well. Finally, we demonstrate the applicability of LYS–AgNCs for real-sample analysis of VB12 with a satisfactory outcome.

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Section: Introductionmentioning

confidence: 99%

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

Sarkar

Saha

Nandi

et al. 2022

ACS Appl. Nano Mater.

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“…Jin and co-workers synthesized Ag 7 NCs with high yield by the reduction of AgNO 3 using meso-2,3-dimercaptosuccinic acid (DMSA) ligands . Blue-light-emitting AgNCs was synthesized by bovine serum albumin (BSA) as capping molecules with high quantum yield . Rotello and co-workers reported polyethylenimine-functionalized cationic AgNCs as potent antimicrobials against multidrug-resistant bacteria .…”

Section: Introductionmentioning

confidence: 99%

“…31 Blue-light-emitting AgNCs was synthesized by bovine serum albumin (BSA) as capping molecules with high quantum yield. 32 Rotello and co-workers reported polyethylenimine-functionalized cationic AgNCs as potent antimicrobials against multidrug-resistant bacteria. 33 Red-emitting AgNCs were synthesized using poly(methacrylic acid) as a template under UV irradiation.…”

Section: ■ Introductionmentioning

confidence: 99%

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model

et al. 2022

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Red-light-emitting silver nanoclusters (AgNCs) are recently emerged as a promising nanoprobe in the field of biomedical applications, because of their attractive properties, including brightness, luminescence stability, and better biocompatibility. In this report, we have developed highly water-soluble redlight-emitting AgNCs by using N-cholyl D-penicilamine (NCPA) as a biosurfactant at above the critical micelle concentration (CMC) at room temperature. Moreover, the NCPA was initially synthesized by demonstrating the reaction between cholic acid and D-penicilamine via a simple coupling reaction strategy. The primary and secondary critical micellar concentration (CMC) of NCPA surfactant was measured using pyrene (1 × 10 −6 M) as a fluorescent probe, and the values were found to be 3.18 and 10.6 mM, respectively. Steady-state fluorescence measurements reveal that the prepared AgNCs shows the excitation and emission maxima at 365 and 672 nm, respectively, with a large Stokes shift (307 nm). The average lifetime measurements and quantum yield of the AgNCs were calculated to be 143.43 ns and 16.34%, respectively. Also, the red luminescent NCPA-templated AgNCs was synthesized in various protic and aprotic polar solvents, among which DMF and DMSO exhibit bright emission at longer wavelength as synthesized in aqueous medium. At higher concentration of AgNO 3 , bright luminescent and highly stable solid AgNCs was obtained with excitation and emission maxima at 607 and 711 nm, respectively. Furthermore, the synthesized AgNCs has been successfully utilized as a fluorescent probe for selective and sensitive detection of S 2− ions at nanomolar level in water samples, showing its potential applicability for the detection of S 2− ions in drinking, river, and tap water samples. Finally, toxicity and bioimaging studies of NCPA-templated AgNCs was demonstrated using zebrafish as in vivo model, showing no significant toxicity up to 200 μL/mL. The AgNCs-stained embryos exhibited red fluorescence with high intensity, which shows that AgNCs are stable in a living system.

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“…Previously it has been reported that the reduced-cysteine residues have strong interactions with silver nanoparticles (AgNPs), which play roles in maintaining its native conformations [12]. Biologically synthesized (B-AgNPs) interact with proteins and form a dynamic B-AgNPs-protein corona which probably influences unfolding and refolding pathway resulting in protein aggregation inhibition and chaperone-like activity [13].…”

Section: Introductionmentioning

confidence: 99%

Investigating Chaperone like Activity of Green Silver Nanoparticles: Possible Implications in Drug Development

et al. 2022

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Protein aggregation and amyloidogenesis have been associated with several neurodegenerative disorders like Alzheimer’s, Parkinson’s etc. Unfortunately, there are still no proper drugs and no effective treatment available. Due to the unique properties of noble metallic nanoparticles, they have been used in diverse fields of biomedicine like drug designing, drug delivery, tumour targeting, bio-sensing, tissue engineering etc. Small-sized silver nanoparticles have been reported to have anti-biotic, anti-cancer and anti-viral activities apart from their cytotoxic effects. The current study was carried out in a carefully designed in-vitro to observe the anti-amyloidogenic and inhibitory effects of biologically synthesized green silver nanoparticles (B-AgNPs) on human serum albumin (HSA) aggregation taken as a model protein. We have used different biophysical assays like thioflavin T (ThT), 8-Anilino-1-naphthalene-sulphonic acid (ANS), Far-UV CD etc. to analyze protein aggregation and aggregation inhibition in vitro. It has been observed that the synthesized fluorescent B-AgNPs showed inhibitory effects on protein aggregation in a concentration-dependent manner reaching a plateau, after which the effect of aggregation inhibition was significantly declined. We also observed meaningful chaperone-like aggregation-inhibition activities of as-synthesized florescent B-AgNPs in astrocytes.

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Protein-activated transformation of silver nanoparticles into blue and red-emitting nanoclusters

Abstract: Protein capping can trigger nanoparticle to nanocluster transformation at elevated pH.

Cited by 17 publications

References 46 publications

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model

Investigating Chaperone like Activity of Green Silver Nanoparticles: Possible Implications in Drug Development

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Protein-activated transformation of silver nanoparticles into blue and red-emitting nanoclusters

Abstract: Protein capping can trigger nanoparticle to nanocluster transformation at elevated pH.

Cited by 17 publications

References 46 publications

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu2+ and Vitamin B12 in Living Cells

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu2+ and Vitamin B12 in Living Cells

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S2– Ions and Bioimaging Application Using Zebrafish Model

Investigating Chaperone like Activity of Green Silver Nanoparticles: Possible Implications in Drug Development

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Product

Resources

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Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

Red-Emitting Silver Nanoclusters for Dual-Mode Detection of Cu²⁺ and Vitamin B₁₂ in Living Cells

N-Cholyl d-Penicilamine Micelles Templated Red Light-Emitting Silver Nanoclusters: Fluorometric Sensor for S^2– Ions and Bioimaging Application Using Zebrafish Model