Protein adsorption and human osteoblast‐like cell attachment and growth on alkylthiol on gold self‐assembled monolayers

Abstract

Protein adsorption and growth of primary human osteoblasts on self-assembled monolayers of alkylthiols on gold (SAMs) with carboxylic acid and hydroxyl and methyl termini were investigated. Single-component SAMs and SAMs patterned by photolithographic techniques were used. Cell growth on patterned SAMs demonstrated preferences for one pattern region in all combinations of alkylthiols, with the hierarchical preference COOH > OH > CH(3). Patterned SAMs and immunochemistry were used to investigate adsorption of fibronectin and albumin with respect to different alkylthiol termini. Fibronectin adsorption from both pure solution and serum containing cell culture medium (SDMEM) followed the sequence COOH > OH > CH(3). Albumin adsorption from pure solution followed the sequence OH > COOH > CH(3); from SDMEM the sequence was CH(3) > OH > COOH. Cell attachment to SAMs with the above termini, after preadsorption with fibronectin, albumin, or mixtures of fibronectin and albumin, was measured. Attachment was maximal on COOH-terminated SAMs precoated with fibronectin. Attachment to COOH was significantly reduced only when fibronectin was omitted from the protein preadsorption solution. On OH and CH(3) SAMs increasing the proportion of albumin in the solution was sufficient to significantly reduce cell attachment. The distribution vinculin and the integrins alpha(5)beta(1) and alpha(v)beta(3) indicated that focal contact formation by cells varied with alkylthiol termini in the following sequence: COOH > OH > CH(3).

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

“…Surface wettability can be altered in many ways such as plasma treatment, surface coating, introduction of hydrophilic or hydrophobic groups by self-assembled monolayers (SAMs) 11,12,14,[22][23][24][25][26] . Among these methods, plasma treatment (radio frequency glow discharge) has a long history of modifying surface wettability.…”

Nanofeatured Titanium Surfaces for Dental Implantology: Biological Effects, Biocompatibility, and Safety

“…Molecular selfassembly allows for the deposition of a self-assembled monolayer onto a substrate and subsequent exposure of an active terminal group that promotes specific biological functions such as the adhesion of protein and cells [94] or bone deposition [95]. This technique sounds promising because it allows obtaining a selective adhesion to the surface.…”

“…The adsorption of fibrinogen and platelet adhesion, for example, is inversely related to the hydrophilicity of the surface [94,131]. In a study, the authors found that cells were unable to adhere to hydrophobic surfaces previously coated with albumin but could easily adhere to hydrophilic surfaces covered with albumin [132].…”

Interaction between acrylic substrates and RAD16-I peptide in its self-assembling

“…Indeed, PEA based copolymers containing hydroxyl groups have already proven to trigger the formation of fibronectin networks on their surfaces, provided that the fraction of the latter be lower than 0.7 [17]. Other studies have also demonstrated that the presence of carboxyl groups enhanced the protein surface interaction [33], which had a straightforward impact on the biological outcome of the substrate [34].…”