Protein Adsorption and Platelet Adhesion on the Surface of an Oxygenator Membrane

Niimi, Yoshinari; Yamane, Shingo; Yamaji, Katsuhiko; Tayama, Eiki; Sueoka, Akinori; Nosé, Yukihiko

doi:10.1097/00002480-199709000-00076

Cited by 18 publications

(27 citation statements)

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“…33 Typical physiological concentrations of the proteins were used throughout the experiment. 34 The assay used for this protein adsorption analysis was the Amido Black assay.…”

Section: Protein Adsorptionmentioning

confidence: 99%

“…The two proteins studied were albumin, which is known to reduce platelet adhesion, 33 and fibrinogen, which is an adhesive protein known to enhance the adhesion and activation of platelets. 38 According to Absolom et al, 39 who studied protein adsorption to polymer particles with different surface energies, protein adsorption decreases as the surface energy of the polymer increases.…”

Section: Protein Adsorptionmentioning

confidence: 99%

See 1 more Smart Citation

Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications

Jones

McColl

Grant

et al. 2000

J. Biomed. Mater. Res.

284

151

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The hemocompatibility of a TiN/TiC/diamond-like carbon (DLC) multilayer structure, deposited on titanium substrates for use as coatings for a heart valve prosthesis, has been studied through the adsorption of blood proteins and the adhesion and attachment of blood platelets. All of the surfaces were characterized by stylus profilometry and water contact angles. The adsorption of albumin and fibrinogen to the surfaces was assessed using the Amido Black assay, whereas platelet attachment was studied by scanning electron microscopy and quantified using stereological techniques. The degree of platelet spreading on the surfaces was seen to correlate with differences in surface energy, indicated from contact angle measurements. The greatest spreading was seen on the more hydrophilic surfaces. When studying protein adsorption to the surfaces, no correlation could be determined between contact angle results and levels of adsorption, although the most hydrophilic surfaces did appear to promote greater amounts of fibrinogen adsorption. Thrombus formation was observed to some degree on all of the surfaces, with the exception of the DLC coating. This coating also promoted less spreading of platelets than the other surfaces. The good hemocompatibility of the DLC coating is attributed to its hydrophobicity and smooth surface, resulting in a higher ratio of albumin to fibrinogen than any of the other surfaces.

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“…33 Typical physiological concentrations of the proteins were used throughout the experiment. 34 The assay used for this protein adsorption analysis was the Amido Black assay.…”

Section: Protein Adsorptionmentioning

confidence: 99%

Section: Protein Adsorptionmentioning

confidence: 99%

Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications

Jones

McColl

Grant

et al. 2000

J. Biomed. Mater. Res.

284

151

View full text Add to dashboard Cite

show abstract

“…The adsorption of albumin (Alb) and fibrinogen (Fg) has been extensively applied for the assessment of blood compatibility of material surfaces [45]. Amido Black assay was reported to be suitable for the determination of proteins that have been adsorbed to solid surfaces [46].…”

Section: Protein Adsorptionmentioning

confidence: 99%

“…6). The results are given in terms of absorbance of the homogenized eluent solution, following removal of the bound dye which is proportional to the amount of bound protein on the surface of the materials [45,52]. The amount of fibrinogen adsorbed on the control PVC surface was significantly higher than the coated PVC surfaces (p < 0.01).…”

Section: Protein Adsorptionmentioning

confidence: 99%

Self-assembled hemocompatible coating on poly (vinyl chloride) surface

Zha

Yue

et al. 2009

Applied Surface Science

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“…193,194 Based on these earlier studies, it is still common practice that CPB circuits are primed with albumin, believing it will bind to the circuit and reduce the binding of other physiologically important proteins such as fibrinogen, and preserving platelet function. 193,194 Despite albumin priming of one group (AR), we reported similar reductions in circulating albumin from the whole blood in both the PR and AR circuits at T1 and T2. Our circuits were Trillium coated, and studies have shown minimized protein binding with this surface coating.…”

Section: -4)mentioning

confidence: 99%

Selenium and oxidative stress modulation during extracorporeal circulation

McDonald¹

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Oxidative stress occurs when there is an imbalance between reactive oxygen and nitrogen species (RNOS) and the antioxidant system. In cardiac surgery patients and the critically ill there is an association between oxidative stress and morbidity and mortality.Extracorporeal circulation (ECC) encompasses cardiopulmonary bypass (CPB) which is used for cardiac surgery; extracorporeal membrane oxygenation (ECMO) which is used for cardio-respiratory support in the critically ill and haemodialysis which is used in patients with intermittent or end-stage renal failure.During ECC, the patient's total blood volume is in direct contact with an artificial surface for extended periods. In addition, there is often pre-existing inflammation, surgical trauma and/or ischemia-reperfusion (I-R) injury, all of which are major activators for the increased production of RNOS. Concurrent decreases in the level of essential antioxidants and trace elements (such as selenium, zinc and copper) occur, which upset redox balance in favour of oxidative stress. The trace element selenium is required for the normal function of the antioxidant enzyme glutathione peroxidase (GPx). Despite in vitro and biochemical data suggesting that reinforcement of antioxidant activity by selenium supplementation might reduce excessive oxidative stress damage, there is conflicting evidence in clinical studies.The reason for failure of antioxidant supplementation in the clinical setting is currently unclear.We postulated that the ECC and its associated interventions such as transfusion, might have a larger impact on trace element loss, antioxidant function and the oxidative stress response than previously recognised. This led to the following questions:  What is the selenium level in healthy Queensland residents, and is this level similar to that reported in other parts of the world? Does a patient's pre-CPB or pre-ECMO circulating selenium level influence their outcome? Does the ECC independently alter circulating selenium levels and the oxidative stress response? Do other interventions during ECC, such as transfusion, alter circulating selenium and oxidative stress levels?iii This thesis focussed on oxidative stress and the trace element selenium, because oxidative stress is associated with poorer outcomes in cardiac and critically ill patients, and decreased selenium is independently associated with poorer outcomes. To answer the research questions outlined above, a series of experiments were designed and conducted, and they generated the following new information.The average selenium level in a Queensland blood donor population was defined at 1.09 µmol/L (Chapter 3). In addition 88.5% of participants had a selenium level lower than the critical level required for maximal glutathione peroxidase activity, which has previously been determined to be between 1.14 -1.27 µmol/L. Furthermore, this study generated novel information that packed red blood cells and buffy coat pooled platelets have very low levels of selenium (≤ 0.3 µmol/L).The effect of car...

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Protein Adsorption and Platelet Adhesion on the Surface of an Oxygenator Membrane

Cited by 18 publications

References 0 publications

Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications

Protein adsorption and platelet attachment and activation, on TiN, TiC, and DLC coatings on titanium for cardiovascular applications

Self-assembled hemocompatible coating on poly (vinyl chloride) surface

Selenium and oxidative stress modulation during extracorporeal circulation

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