Protein Adsorption at Nanopatterned Surfaces Studied by Quartz Crystal Microbalance with Dissipation and Surface Plasmon Resonance

Kristensen, Stine H.; Pedersen, Gitte A.; Nejsum, Lene N.; Sutherland, Duncan S.

doi:10.1021/jp4038528

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…A few characterization methods such as reflectivity (Su et al, 1998b(Su et al, , 2016Raghuwanshi et al, 2017a,b), ellipsometer, atomic force microscope (AFM), surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation (QCM-D) can measure the adsorbed protein layer thickness at the nanometer scale required. In particular, QCM-D can kinetically monitor the biomolecules sorption process by measuring the adsorbed protein mass at an interface in nanograms (Kristensen et al, 2013;Luan et al, 2017). QCM-D enables the control of temperature, ionic strength and pH environment.…”

Section: Introductionmentioning

confidence: 99%

Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Raghuwanshi

Browne

et al. 2020

Front. Bioeng. Biotechnol.

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A pH dependent reversible sponge like behavior of a bovine serum albumin (BSA) nanolayer adsorbed at the gold-saline interface is revealed by quartz crystal microbalance with dissipation (QCM-D), atomic force microscope (AFM) and contact angle measurements. During the saline rinsing cycles, the BSA layer adsorbs water molecules at pH 7.0 and releases them at pH 4.5. The phenomenon remains constant and reproducible upon multiple rinsing cycles. The BSA layer thickness also increases upon rinsing with saline at pH 7.0 and reverses back to its original thickness at pH 4.5. Varying ionic strength with water further desorbs more water molecules from the BSA layer, which decreases its mass and thickness. However, upon both pH and ionic strength changes, all the BSA molecules remain adsorbed irreversibly at the gold interface and only the sorption of water molecules occurs. The study aims at engineering high efficiency pH-responsive biodiagnostics and drug delivery systems.

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Section: Introductionmentioning

confidence: 99%

Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Raghuwanshi

Browne

et al. 2020

Front. Bioeng. Biotechnol.

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“…By contrast, other popular sensor techniques such as surface plasmon resonance (SPR) typically require metal-coated surfaces that are not compatible with membrane fabrication [ 21 , 22 ]. At the same time, fabricating nanostructures on QCM-D sensing platforms requires delicate attention to how nanostructure-associated hydrodynamic effects influence measurement responses [ 23 , 24 , 25 ]. Another promising measurement option involves utilizing nanoplasmonic sensors whereby the nanostructures not only contribute to the sensor geometry but also play an active role as the transducing element [ 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Ferhan

Junren

Jackman

et al. 2017

Sensors

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The integration of supported lipid membranes with surface-based nanoplasmonic arrays provides a powerful sensing approach to investigate biointerfacial phenomena at membrane interfaces. While a growing number of lipid vesicles, protein, and nucleic acid systems have been explored with nanoplasmonic sensors, there has been only very limited investigation of the interactions between solution-phase nanomaterials and supported lipid membranes. Herein, we established a surface-based localized surface plasmon resonance (LSPR) sensing platform for probing the interaction of dielectric nanoparticles with supported lipid bilayer (SLB)-coated, plasmonic nanodisk arrays. A key emphasis was placed on controlling membrane functionality by tuning the membrane surface charge vis-à-vis lipid composition. The optical sensing properties of the bare and SLB-coated sensor surfaces were quantitatively compared, and provided an experimental approach to evaluate nanoparticle–membrane interactions across different SLB platforms. While the interaction of negatively-charged silica nanoparticles (SiNPs) with a zwitterionic SLB resulted in monotonic adsorption, a stronger interaction with a positively-charged SLB resulted in adsorption and lipid transfer from the SLB to the SiNP surface, in turn influencing the LSPR measurement responses based on the changing spatial proximity of transferred lipids relative to the sensor surface. Precoating SiNPs with bovine serum albumin (BSA) suppressed lipid transfer, resulting in monotonic adsorption onto both zwitterionic and positively-charged SLBs. Collectively, our findings contribute a quantitative understanding of how supported lipid membrane coatings influence the sensing performance of nanoplasmonic arrays, and demonstrate how the high surface sensitivity of nanoplasmonic sensors is well-suited for detecting the complex interactions between nanoparticles and lipid membranes.

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“…Toward this goal, there have been extensive efforts aimed at coupling QCM-D measurements with SPR, − ellipsometry, , and reflectometry − in order to characterize processes such as vesicle adsorption, protein binding, and amphipathic, α-helical (AH) peptide-mediated rupture of adsorbed lipid vesicles. One key advantage of combined measurement approaches is that several independent physical parameters can be measured simultaneously, thereby enabling a more detailed understanding of complex biological processes .…”

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confidence: 99%

Integration of Quartz Crystal Microbalance-Dissipation and Reflection-Mode Localized Surface Plasmon Resonance Sensors for Biomacromolecular Interaction Analysis

2016

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The combination of label-free, surface-sensitive measurement techniques based on different physical principles enables detailed characterization of biomacromolecular interactions at solid-liquid interfaces. To date, most combined measurement systems have involved experimental techniques with similar probing volumes, whereas the potential of utilizing techniques with different surface sensitivities remains largely unexplored, especially for data interpretation. Herein, we report a combined measurement approach that integrates a conventional quartz crystal microbalance-dissipation (QCM-D) setup with a reflection-mode localized surface plasmon (LSPR) sensor. Using this platform, we investigate vesicle adsorption on a titanium oxide-coated sensing substrate along with the amphipathic, α-helical (AH) peptide-induced structural transformation of surface-adsorbed lipid vesicles into a supported lipid bilayer (SLB) as a model biomacromolecular interaction. While the QCM-D and LSPR signals both detected mass uptake arising from vesicle adsorption, tracking the AH peptide-induced structural transformation revealed more complex measurement responses based on the different surface sensitivities of the two techniques. In particular, the LSPR signal recorded an increase in optical mass near the sensor surface which indicated SLB formation, whereas the QCM-D signals detected a significant loss in net acoustic mass due to excess lipid and coupled solvent leaving the probing volume. Importantly, these measurement capabilities allowed us to temporally distinguish the process of SLB formation at the sensor surface from the overall structural transformation process. Looking forward, these label-free measurement capabilities to simultaneously probe adsorbates at multiple length scales will provide new insights into complex biomacromolecular interactions.

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Protein Adsorption at Nanopatterned Surfaces Studied by Quartz Crystal Microbalance with Dissipation and Surface Plasmon Resonance

Cited by 13 publications

References 33 publications

Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Reversible pH Responsive Bovine Serum Albumin Hydrogel Sponge Nanolayer

Probing the Interaction of Dielectric Nanoparticles with Supported Lipid Membrane Coatings on Nanoplasmonic Arrays

Integration of Quartz Crystal Microbalance-Dissipation and Reflection-Mode Localized Surface Plasmon Resonance Sensors for Biomacromolecular Interaction Analysis

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