Protein adsorption behaviour of ionogenic poly(HEMA) membranes: a fluorescence study

Kiremitçi, Menemşe; Gök, Elmas; Ateş, Serdar

doi:10.1163/156856294x00419

Cited by 13 publications

(7 citation statements)

References 21 publications

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“…P(HEMA-acrylic acid, AA) [11]. In addition, the same correlation was found for the adsorption of plasma proteins to these PHEMA surfaces [27]. Similar results were obtained by several research groups.…”

Section: Resultssupporting

confidence: 86%

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Karakeçili¹,

Gümüşderelı́oğlu²

2002

Journal of Biomaterials Science, Polymer Edition

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In this study, interactions of widely-used polymeric biomaterials, i.e. poly(hydroxyethyl methacrylate) (PHEMA) and its copolymer with dimethylaminoethyl methacrylate (PHEMA-20% DMAEMA), polyurethane (PU), polypropylene (PP), poly(vinyl chloride) (PVC), and poly(lactide-glycolide) (PLGA), with three pathogenic bacteria and one nonpathogen were investigated comparatively with the adhesion of two tissue cells in different morphologies, i.e. fibroblast-like baby hamster kidney (BHK 21) cells and epithelial Madine Darby kidney (MDBK) cells. Biomaterials were prepared in the membrane form by bulk polymerization or solvent casting. Surface characterization studies showed that these polymers have different surface free energies in the range of 26.9-63.1 erg cm(-2) and they have smooth surfaces. The bacteria used were; Escherichia coli ATCC 25922, Staphylococcus epidermidis ATCC 12228, Staphylococcus aureus, and Lactobacillus acidophilus B-13. Initial adhesion of bacteria to the polymeric surfaces was examined under static conditions and in a laminar flow cell. The adhesion behaviour of S. aureus and S. epidermidis was found independent of the polymeric surface hydrophobicity. However, the percentage of attached E. coli decreased when increasing the surface free energy of the polymer, while L. acidophilus showed just the opposite behaviour. The comparative results indicated that the adhesion of BHK and MDBK cell was lowest on the most hydrophilic PHEMA surface and highest on the most hydrophobic PP surface. In contrast to the case of bacterial adhesion, no relationship was found between polymer hydrophobicity and mammalian cell adherence.

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Section: Resultssupporting

confidence: 86%

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Karakeçili¹,

Gümüşderelı́oğlu²

2002

Journal of Biomaterials Science, Polymer Edition

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“…22 This finding suggests that some of the proteins reveal their own specific affinity to charged surfaces. Therefore, in order to investigate the patterns of adsorption to the charged surface revealed by individual proteins, individual bands were identified by immunoblotting.…”

Section: Resultsmentioning

confidence: 98%

“…In accord with a number of reports, introducing the charge groups to materials may provide changes in the protein uptake from plasma due to electrostatic interactions. [20][21][22] As listed in Table I, all samples generated negative zeta potentials, and the amount of potentials increased in the order of PVAú Cuprophane C-PVA S-PVA. Basically, non-heat treated PVA gives very small zeta potentials, because it has a diffuse surface due to the absent of crystallinites.…”

Section: Sds-polyacrylamide Gel Electrophoresis (Sds-page)mentioning

confidence: 99%

Plasma protein adsorption to anion substituted poly(vinyl alcohol) membranes

et al. 2003

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Anion-substituted poly(vinyl alcohol) (PVA) membranes, carboxymethylated PVA (C-PVA), and sulfonated PVA (S-PVA) were prepared and the effects of these substitutions on the plasma protein adsorption were studied by one-and two-dimensional gel electrophoresis and immunoblotting. When Cuprophane was used as a negative control, the amount of total proteins bound to samples decreased in the order Cuprophane * PVA * C-PVA * S-PVA, which we attribute to the effects of the surface characteristics of the samples, such as their surface tensions and electrostatic properties, on the adsorption of proteins to the surfaces of the materials. The results revealed that albumin was the most abundant protein in all the samples. The proportion of adsorbed fibrinogen to S-PVA exceeded those of PVA and C-PVA, whereas S-PVA exhibited the lowest IgG adsorption affinity among the samples we studied.

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“…Many polymers in clinical use, however, are not originally designed so that they activate platelets and the complement system and are relatively unstable to enzymatic, hydrolytic, and oxidative stress such as superoxide or peroxide [3,4]. In biomedical polymer research, it is believed that the initial adsorption of protein from blood onto a polymer surface has a great influence on several biological responses such as homeostasis, complement activation, and platelet adhesion [5,6]. That is, adsorption of proteins such as albumin, immunoglobulin, and fibrinogen on the polymers plays a very important role, because the type and conformation of the adsorbed proteins influence the activation of platelets, mediating all the events leading to thrombus formation.…”

mentioning

confidence: 99%

Albumin adsorption to surface of annealed fluorinated polyimide

Kawakami

Takahashi

Nagaoka

et al. 2001

Polymers for Advanced Techs

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Albumin adsorption to a surface of fluorinated polyimide which was essentially created only by annealing treatment was investigated. After the polyimide surfaces were annealed, the morphology change of the surface was determined using atomic force microscopy and the surface properties were analyzed by contact angle measurements and X-ray photoelectron spectroscopy. It was found that albumin adsorption on the surface strongly depended on the annealing temperature and time and decreased with annealing. The amount of albumin adsorption to the polyimide annealed at 250°C for 100 hr was six-fold smaller than that of polystyrene and polydimethylsiloxane. A decrease of surface free energy due to a decline in the polar and hydrogen bonding components and an increase in hydrophobicity on the polyimide surface by annealing explain the albumin adsorption results. Annealed Fluorinated Polyimide / 245 FIGURE 2. Amount of adsorbed BSA on polyimide surface at 36.5°C (ionic strength: 0.17, BSA: 1.0 mg/ ml): (a) 6FDA-DDS; (b) 6FDA-6FAP.

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Protein adsorption behaviour of ionogenic poly(HEMA) membranes: a fluorescence study

Cited by 13 publications

References 21 publications

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Comparison of bacterial and tissue cell initial adhesion on hydrophilic/hydrophobic biomaterials

Plasma protein adsorption to anion substituted poly(vinyl alcohol) membranes

Albumin adsorption to surface of annealed fluorinated polyimide

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