Protein adsorption kinetics in charged agarose gels: Effect of agarose content and modeling

Schirmer, Emily B.; Carta, Giorgio

doi:10.1002/aic.11668

Cited by 15 publications

(14 citation statements)

References 31 publications

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“…[176][177][178][179] In gels with low ϕ, drag is predominantly caused by viscous flow of solvent against the pore walls, so this is typically the only contribution included in models of diffusion. 180 It is clear, however, that adsorption, 181,182 electrostatic forces 181,183 and direct collisions with the pore wall 184,185 may further impede diffusion, particularly if the pore is very narrow, or the solute is charged 186,187 or bulky. [188][189][190] In addition, drag may be amplified if the gel fibres are themselves ionised, 187,191 or present a rough or corrugated surface to the diffusing fluid.…”

Section: Swelling Effectsmentioning

confidence: 99%

Gels with sense: supramolecular materials that respond to heat, light and sound

2016

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. (2016) 'Gels with sense : supramolecular materials that respond to heat, light and sound.', Chemical society reviews., 45 (23). pp. 6546-6596. Further information on publisher's website:https://doi.org/10.1039/C6CS00435KPublisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. Advances in the field of supramolecular chemistry have made it possible, in many situations, to reliably engineer soft materials to address a specific technological problem. Particularly exciting are "smart" gels that undergo reversible physical changes on exposure to remote, non-invasive environmental stimuli. This review explores the development of gels which are transformed by heat, light and ultrasound, as well as other mechanical inputs, applied voltages and magnetic fields. Focusing on small-molecule gelators, but with reference to organic polymers and metal-organic systems, we examine how the structures of gelator assemblies influence the physical and chemical mechanisms leading to thermo-, photo-and mechano-switchable behaviour. In addition, we evaluate how the unique and versatile properties of smart materials may be exploited in a wide range of applications, including catalysis, crystal growth, ion sensing, drug delivery, data storage and biomaterial replacement.

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Section: Swelling Effectsmentioning

confidence: 99%

Gels with sense: supramolecular materials that respond to heat, light and sound

2016

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“…h -1 in order to avoid any temperature fluctuations due to sample heating during the magnetic field pulse gradients. Adsorption kinetics IP adsorption isotherm in AC gel was determined following literature methods 25 . Small hydrogel samples were suspended in excess of IP solution with different concentrations and stirred for 8 h at 37°C.…”

Section: Self-diffusion Coefficients Were Measured By Diffusion Ordermentioning

confidence: 99%

“…At this stage, we propose a mathematical model accounting for the steps described above starting from chromatographic studies 32,64 . The adsorbed IP amount is given by q, as determined from the adsorption Langmuir isotherm (Figure 4) 25 . The isotherm is highly favorable at low drug concentration.…”

Section: Modeling Ip Diffusion Coefficients In Gelmentioning

confidence: 99%

“…This is mainly due to the fact that theoretical studies on drug delivery systems are centered on pure-Fickian diffusion with degradation and swelling of polymers, not considering any other contribution 23,24 . In order to put order in this matter we decided to change the common point of view and consider the large amount of theories and models already developed for hydrogels used in chromatography 17,25,26 .…”

Section: Introductionmentioning

confidence: 99%

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On the parallelism between the mechanisms behind chromatography and drug delivery: the role of interactions with a stationary phase

Rossi

Castiglione

Salvalaglio

et al. 2017

Phys. Chem. Chem. Phys.

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A huge number of studies and works in drug delivery literature are focused on understanding and modeling transport phenomena, the pivotal point for a good device design. The rationalization of all phenomena involved is fundamental, but several concerns arise leaving many issues unsolved. In order to change point of view we decided to focus our attention on parallelisms between two fields that seem to be very far each other: chromatography and drug release. Taking advantages on the studies conducted by many researchers with chromatographic columns we decided to explain all the phenomena involved in drug delivery considering sodium ibuprofen molecules (IP) as analytes and hydrogel as stationary phase. In particular we considered not only diffusion, but also drug-polymer interactions as adsorption on the stationary phase and drug-drug interactions as analytes aggregation. Hydrogel investigated is a promising formulation made of agarose and carbomer 974p (AC) loaded with IP, a non-steroidal common anti-inflammatory drugs. The self-diffusion coefficient of IP in AC formulations was measured by using an innovative method based on Magic Angle Spinning NMR spectroscopic technique to produce High Resolution (liquid-like) spectra.This method (HR-MAS NMR) is used in combination with pulsed field gradient spin echo (PGSE) liquid-state techniques. The model predictions satisfactorily match with experimental data obtained in water and the gel environment, indicating that the model presented here, despite its simplicity, is able to describe the key phenomena governing the device behavior and could be used to rationalize the experimental activity.!

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“…As shown, for example, by Schirmer and Carta (2008), at the macroscopic level the mechanisms of pore and solid diffusion are not directly distinguishable because both lead to uptake curves (amount of protein adsorbed as a function of time) that have nearly the same shape…”

Section: Intraparticle Transportmentioning

confidence: 99%

Protein Adsorption in Tentacle-Type Anion Exchangers and the Influence of Process Related Fouling

Corbett¹

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i AbstractThe physical and protein adsorption properties of Fractogel TMAE, a tentacle-type anion exchange resin, were investigated for both virgin and used samples to determine the influence of process related fouling. The resin average particle diameter was found to vary between 70 and 72 µm for different resin lots. Inverse size exclusion chromatography (iSEC) indicated a bimodal distribution of pore sizes, with some large pores, 40 nm in radius. occupying about 9% of the particle volume and much smaller pores, with radius between 4 and 5 nm, occupying 74% of the particle volume. Similar results were obtained by iSEC for resin samples fouled by process use, indicating that the core structure of these particles is unchanged. Transmission electron micrographs (TEM) showed that the resin backbone has a microgranular structure and that the same structure persists. In this case, however, a dense layer, approximately 0.5 µm thick, was also seen at the particle exterior surface of the fouled particles. The equilibrium binding capacity of BSA was determined to be 178±1 mg/mL for both virgin and fouled samples, respectively, while the corresponding values for Thyroglobulin were 95±4 and 25±2 mg/mL. The BSA adsorption kinetics was also 2-3 fold slower for the fouled resin, but much larger differences between virgin and fouled resin were seen for the much larger Thyroglobulin. Based on the shape of intraparticle protein concentration profiles determined by confocal laser scanning microscopy (CLSM), the protein transport mechanism was determined to be "solid diffusion" for both virgin and fouled resin samples and proteins. However, transport of Thyroglobulin was much slower for the fouled sample as a result of its larger size and increased diffusional hindrance in the fouled surface layer. The key conclusion is that fouling occurs through the formation of a think but very dense skin layer that greatly affects the transport kinetics of large proteins.ii Acknowledgements

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Protein adsorption kinetics in charged agarose gels: Effect of agarose content and modeling

Cited by 15 publications

References 31 publications

Gels with sense: supramolecular materials that respond to heat, light and sound

Gels with sense: supramolecular materials that respond to heat, light and sound

On the parallelism between the mechanisms behind chromatography and drug delivery: the role of interactions with a stationary phase

Protein Adsorption in Tentacle-Type Anion Exchangers and the Influence of Process Related Fouling

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