Protein Adsorption on Nanocrystalline TiO₂ Films:  An Immobilization Strategy for Bioanalytical Devices

Abstract

We have investigated the use of optically transparent, nanoporous TiO(2) films as substrates for protein immobilization. Immobilization on such films may be readily achieved from aqueous solutions at 4 °C. The nanoporous structure of the film greatly enhances the active surface area available for protein binding (by a factor of 150 for a 4-μm-thick film). We demonstrate that the redox state of immobilized cytochrome c may be modulated by the application of an electrical bias potential to the TiO(2) film and that the fluorescence yield of immobilized fluorophore-labeled maltose-binding protein may be used to monitor specifically maltose concentration. We conclude that nanoporous TiO(2) films may be useful both for basic studies of protein/electrode interactions and for the development of array-based bioanalytical devices employing both optical and electrochemical signal transduction methodologies.

Analytical strategies for phosphoproteomics

“…For some years, TiO 2 was used in bioelectrochemical studies of protein functions using the adsorption of proteins to TiO 2 films [63]. More interestingly for phosphoproteomic studies, TiO 2 was shown to have affinity for phosphate ions from aqueous solutions [64][65][66][67][68][69][70][71].…”

Engineered Derivatives of Maltose-Binding Protein

“…Topoglidis et al used a nanocrystallin TiO 2 surface to immobilize a fluorophore-labeled MBP and showed a change in fluorescence in response to maltose (Topoglidis et al, 1998). Dattelbaum et al incorporated a fluorophore-labeled MBP into a sol-gel silica matrix, and demonstrated fluorescence change in response to maltose at close to the same molar sensitivity as MBP in solution (Dattelbaum et al, 2009).…”

Characterization of Titanium Oxide Nanoparticles Obtained by Hydrolysis Reaction of Ethylene Glycol Solution of Alkoxide

“…Several various applications of TiO 2 particles have been studied in recent years, as photocatalysts and to solar cells, UV-shielding materials and electric devices [3][4][5][6]. For these applications, development of a simple synthesis method to obtain TiO 2 nanoparticles with highly homogeneous dispersion has been required [7,8].…”