Protein Adsorption on SiO2-CaO Bioactive Glass Nanoparticles with Controllable Ca Content

Abstract

Bioactive glass nanoparticles (BGNs) are emerging multifunctional building blocks for various biomedical applications. In this study, the primary aim was to develop monodispersed binary SiO2-CaO BGNs with controllable Ca content. We successfully synthesized such spherical BGNs (size ~110 nm) using a modified Stöber method. Our results showed that the incorporated Ca did not significantly affect particle size, specific surface area, and structure of BGNs. Concentrations of CaO in BGN compositions ranging from 0 to 10 mol% could be obtained without the gap between actual and nominal compositions. For this type of BGNs (specific surface area 30 m2/g), the maximum concentration of incorporated CaO appeared to be ~12 mol%. The influence of Ca content on protein adsorption was investigated using bovine serum albumin (BSA) and lysozyme as model proteins. The amount of adsorbed proteins increased over time at the early stage of adsorption (<2 h), regardless of glass composition and protein type. Further incubation of BGNs with protein-containing solutions seemed to induce a reduced amount of adsorbed proteins, which was more significant in BGNs with higher Ca content. The results indicate that the Ca content in BGNs is related to their protein adsorption behavior.

Combination of Selective Etching and Impregnation toward Hollow Mesoporous Bioactive Glass Nanoparticles

“…The particles were monodispersed and exhibited uniform spherical shape with a particle size of ~400 nm, as shown in SEM images ( Figure 2 a), which is a typical morphology of nanoparticles synthesized by using the Stöber method. The particle size of SiO 2 NPs can be controlled by tuning processing parameters (e.g., concentrations of catalysts and precursors, water/ethanol ratio) [ 19 ]. Here we selected SiO 2 NPs with a size of 400 nm as the hard template.…”

“…All chemicals used in this study were purchased from Sigma-Aldrich (Darmstadt, Germany) without further purification. Silica nanoparticles (SiO 2 NPs) were synthesized using a modified Stöber method as described in our previous study [ 19 ]. In a typical synthesis, solution A was prepared by adding 3 mL of tetraethyl orthosilicate (TEOS) in ethanol (25 mL), while solution B was prepared by mixing ammonium hydroxide solution (4.5 mL, 28 wt%), ethanol (8.2 mL), and deionized water (12.4 mL).…”

“…The as-synthesized SiO 2 NPs were dried at 60 °C for further experiments. The size of SiO 2 NPs could be adjusted by tuning concentrations of used water, ethanol, and ammonia [ 19 ]. However, in this study, only 400 nm SiO 2 NPs were used as the hard template.…”

Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications

“…When biomaterials are in contact with body fluids, they get surrounded by proteins. In a short time, proteins are adsorbed on the surface of biomaterials, and the subsequent biological interactions with the materials depend on the characteristics of the protein attachment [ 59 , 60 ]. Numerous parameters affect the interaction between a biomaterial and proteins, including surface chemical composition, biomaterial characteristics (particle size, surface charge, hydrophobicity, crystallinity, and surface topography), and protein properties (dimension and molecular weight) [ 59 ].…”

Synthesis, Structural, Morphological and Thermal Characterization of Five Different Silica-Polyethylene Glycol-Chlorogenic Acid Hybrid Materials

“…In recent years, silica-based materials, with particular reference to organic-inorganic hybrids [ 4 , 5 , 6 ] and calcium bioactive glasses [ 7 , 8 , 9 ], have received increasing attention due to their relevant biological properties. In particular, they commonly show high biocompatibility, thus providing positive biological effects because their reaction products are able to promote cell–material interactions and cell invasion [ 10 ].…”