Protein adsorption on tailored substrates: long-range forces and conformational changes

Bellion, M.; Santen, Ludger; Mantz, Hubert; Hähl, Hendrik; Quinn, Anthony S.; Nagel, Armin M.; Gilow, Christoph; Weitenberg, Christof; Schmitt, Yvonne; Jacobs, Karin

doi:10.1088/0953-8984/20/40/404226

Cited by 55 publications

(82 citation statements)

References 43 publications

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“…This result is consistent with what has been observed in the experiments from single protein absorption when conformational changes occur 36 . Moreover, our analysis for competitive adsorption shows, as for T = 300 K, that the linear regime of Fib adsorption coincides with the end of the desorption of the Alb and the beginning of desorption of IgG.…”

Section: Effect Of Energy Depletionsupporting

confidence: 93%

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

2013

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It is now well accepted that cellular responses to materials in a biological medium reflect greatly the adsorbed biomolecular layer, rather than the material itself. Here, we study by molecular dynamic simulations the competitive protein adsorption on a surface (Vroman effect), i.e. the non-monotonic behavior of the amount of protein adsorbed on a surface in contact with plasma as a function of contact time and plasma concentration. We find a complex behavior, with regimes during which small and large proteins are not necessarily competing between them, but are both competing with others in solution. We show how the effect can be understood, controlled and inverted.

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Section: Effect Of Energy Depletionsupporting

confidence: 93%

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

2013

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“…41,42 Based on Monte-Carlo simulations featuring surface processes such as surface mobility and conformational changes, the distinctions were invoked by the influence of the vdW-interactions on the time scale of these processes. 43 X-ray reflectivity experiments corroborated these findings. 44 Recent studies discovered that the influence of the subsurface material on adhesion is sensed by larger biological objects, too: The unspecific adhesion of bacteria from the Staphylococcus genus is affected by the properties of the subsurface material, as could be shown by AFM force spectroscopy measurements.…”

Section: Interactions In Biological Systemssupporting

confidence: 56%

Is adhesion superficial? Silicon wafers as a model system to study van der Waals interactions

Loskill¹,

Hähl²,

Faidt³

et al. 2012

Advances in Colloid and Interface Science

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Adhesion is a key issue for researchers of various fields, it is therefore of uppermost importance to understand the parameters that are involved. Commonly, only surface parameters are employed to determine the adhesive forces between materials. Yet, van der Waals forces act not only between atoms in the vicinity of the surface, but also between atoms in the bulk material. In this review, we describe the principles of van der Waals interactions and outline experimental and theoretical studies investigating the influence of the subsurface material on adhesion. In addition, we present a collection of data indicating that silicon wafers with native oxide layers are a good model substrate to study van der Waals interactions with coated materials.

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“…Recently, a similar influence was detected on the adsorption kinetics of proteins [11][12][13] and the adhesion of bacteria [14]. These experiments used materials whose contribution to the potential had been tuned by means of surface stratification.…”

Section: Introductionmentioning

confidence: 66%

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

Loskill

Puthoff

Wilkinson

et al. 2013

J. R. Soc. Interface.

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Surface energies are commonly used to determine the adhesion forces between materials. However, the component of surface energy derived from long-range forces, such as van der Waals forces, depends on the material's structure below the outermost atomic layers. Previous theoretical results and indirect experimental evidence suggest that the van der Waals energies of subsurface layers will influence interfacial adhesion forces. We discovered that nanometre-scale differences in the oxide layer thickness of silicon wafers result in significant macroscale differences in the adhesion of isolated gecko setal arrays. Si/SiO 2 bilayer materials exhibited stronger adhesion when the SiO 2 layer is thin (approx. 2 nm). To further explore how layered materials influence adhesion, we functionalized similar substrates with an octadecyltrichlorosilane monolayer and again identified a significant influence of the SiO 2 layer thickness on adhesion. Our theoretical calculations describe how variation in the SiO 2 layer thickness produces differences in the van der Waals interaction potential, and these differences are reflected in the adhesion mechanics. Setal arrays used as tribological probes provide the first empirical evidence that the 'subsurface energy' of inhomogeneous materials influences the macroscopic surface forces.

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Protein adsorption on tailored substrates: long-range forces and conformational changes

Cited by 55 publications

References 43 publications

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

Understanding and modulating the competitive surface-adsorption of proteins through coarse-grained molecular dynamics simulations

Is adhesion superficial? Silicon wafers as a model system to study van der Waals interactions

Macroscale adhesion of gecko setae reflects nanoscale differences in subsurface composition

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