Protein and allergen content of various natural latex articles

Baur, Xaver; Chen, Z.; Raulf‐Heimsoth, Monika; Degens, Paul O.

doi:10.1111/j.1398-9995.1997.tb01046.x

Cited by 49 publications

(31 citation statements)

References 21 publications

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“…The manufacturing process may lead to denaturing of proteins in raw latex, and it has been reported that the allergen profiles differed between finished products and the raw materials [25, 26]. Previous studies also documented that the total protein level does not necessarily correlate with the allergen content [24, 27, 28]. There was no known ‘safety margin’ for the level of total latex protein with regard to the allergenicity of latex gloves, unless we measure the true IgE-binding allergen directly.…”

Section: Discussionmentioning

confidence: 99%

Identification of Hevamine and Hev b 1 as Major Latex Allergens in Taiwan

Lee

Chen

Lin³

et al. 2005

Int Arch Allergy Immunol

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Background: Proteins from latex gloves have been documented to trigger occupational latex allergy among health care workers. Allergen characterization of latex glove extract has never been studied in Taiwan. This study aimed to identify allergenic proteins from latex gloves. Methods: Crude extracts of latex gloves were prepared with phosphate-buffered saline and 20 medical workers with a history of latex allergy were enrolled in this study. The specific IgE antibody was determined by the Pharmacia CAP system and in-house enzyme-linked immunoassay and immunoblotting. The target proteins were excised from two-dimensional PAGE and analyzed by electrospray ionization tandem mass spectrometry. Results: Immunoblotting of glove extracts revealed three IgE-binding proteins at a molecular mass of 45, 30 and 14 kDa. Peptide mass fingerprinting revealed that the protein at 45 kDa, which was recognized by 10% (2/20) of atopic sera tested, was an allergenic lipolytic esterase from Hevea brasiliensis (Hev b 13). The 30- and 14-kDa proteins, which were recognized by 55% (11/20) and 85% (17/20) of patients’ sera, were found to be hevamine and rubber elongation factor (Hev b 1), respectively. Conclusions: Our results indicated that hevamine and Hev b 1 are the major allergens from latex gloves in Taiwan, which differs from the reports in Western countries.

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Section: Discussionmentioning

confidence: 99%

Identification of Hevamine and Hev b 1 as Major Latex Allergens in Taiwan

Lee

Chen

Lin³

et al. 2005

Int Arch Allergy Immunol

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“…Studien beschrieben eine Prävalenz der Sensibilisierung gegen Naturlatex im medizinischen Berufen von bis zu 17% [7]. Hauptallergenträger sind gepuderte Latexhandschuhe [2,6]. Die Zahl der bestätigten BK 5101-Fälle stieg von etwa 300 im Jahr 1995 auf etwa 900 im Jahr 1998.…”

Section: Hintergrund Und Fragestellungunclassified

Berufsbedingte Latexallergie

Nienhaus

Kromark

Raulf‐Heimsoth

et al. 2007

Trauma Berufskrankh

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“…Extracts from latex products were prepared according to the method of Baur et al [9]. Briefly, 5 g of NRL glove materials were cut into small pieces (approximately 1–2 cm 2 ) and extracted in PBS (pH 7.4) by shaking for 2 h at 37°C (5 g of latex glove material in 50 ml of extraction buffer).…”

Section: Methodsmentioning

confidence: 99%

Development of a Monoclonal Antibody-Based Sandwich ELISA for Detection of the Latex Allergen Hev b 1

Raulf‐Heimsoth

Sander²,

Chen³

et al. 2000

Int Arch Allergy Immunol

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Background: Natural rubber latex (NRL) products are complex mixtures consisting of different allergenic components. Among them, Hev b 1 belongs to the important and well-characterized ones. To quantify the relevant allergen Hev b 1 in NRL products, a two-site monoclonal antibody (mAb)-based assay was developed. Methods: Two Hev b 1-specific mAbs with different epitope recognition and ability to bind simultaneously to an Hev b 1 molecule were used in the study. Both mAbs (II4F9 and II4G9) were enriched by in vitro production in a modular minifermenter and affinity purified. Wells of micro-ELISA plates coated with captured mAb II4G9 were incubated with samples containing Hev b 1. Bound Hev b 1 was detected by a combination of biotinylated mAb II4F9 as detection antibody and peroxidase-labeled avidin. Results: The optimized sandwich ELISA was highly reproducible in the linear range of the standard curve and Hev b 1 concentrations ranging from 12.5 to 400 ng/100 μl could be detected. The assay was suitable for the detection of Hev b 1 concentrations in latex sap and latex products, e.g. gloves, with a detection limit of 1.25 μg of Hev b 1/g of rubber. In a preliminary study with five different brands of latex gloves, Hev b 1 concentrations were found to be in the range of 18–40 μg per gram of rubber material, corresponding to 2–4% of the total extractable protein content in latex glove extracts. Conclusions: A sensitive sandwich assay was developed to quantify the latex allergen Hev b 1. This assay can be used to standardize latex extracts with regard to the content of the major allergen Hev b 1.

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Protein and allergen content of various natural latex articles

Cited by 49 publications

References 21 publications

Identification of Hevamine and Hev b 1 as Major Latex Allergens in Taiwan

Identification of Hevamine and Hev b 1 as Major Latex Allergens in Taiwan

Berufsbedingte Latexallergie

Development of a Monoclonal Antibody-Based Sandwich ELISA for Detection of the Latex Allergen Hev b 1

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