Protein and DNA Oxidation in Different Anatomic Regions of Rat Brain in a Mimetic Ageing Model

Yanar, Karolin; Aydın, Seval; Çakatay, Ufuk; Mengi, Murat; Büyükpınarbaşılı, Nur; Atukeren, Pınar; Sitar, Mustafa Erinç; Sönmez, Aslı; Uslu, Ezel

doi:10.1111/j.1742-7843.2011.00756.x

Cited by 67 publications

(42 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, understanding the causes and mechanisms associated with age‐related cardiovascular diseases is of considerable importance. Many experimental indications have shown that the characteristics of premature ageing induced by chronic d ‐galactose exposure are similar to those in natural ageing in rodents . Galactose is a monosaccharide sugar, and although it has two forms, the d and l forms, the body can metabolize only the d form of galactose.…”

Section: Introductionmentioning

confidence: 99%

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

Bo-Htay

Palee

Apaijai

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Ageing is a strong independent risk factor for disability, morbidity and mortality. Post‐mitotic cells including those in the heart are a particular risk to age‐related deterioration. As the occurrence of heart disease is increasing rapidly with an ageing population, knowledge regarding the mechanisms of age‐related cardiac susceptibility and possible therapeutic interventions needs to be acquired to prevent advancing levels of heart disease. To understand more about the ageing heart, numerous aged animal models are being used to explore the underlying mechanisms. Due to time‐consuming for investigations involving naturally aged animals, mimetic ageing models are being utilized to assess the related effects of ageing on disease occurrence. d‐galactose is one of the substances used to instigate ageing in various models, and techniques involving this have been widely used since 1991. However, the mechanism through which d‐galactose induces ageing in the heart remains unclear. The aim of this review was to comprehensively summarize the current findings from in vitro and in vivo studies on the effects of d‐galactose‐induced ageing on the heart, and possible therapeutic interventions against ageing heart models. From this review, we hope to provide invaluable information for future studies and based on the findings from experiments involving animals, we can inform possible therapeutic strategies for the prevention of age‐related heart diseases in clinical settings.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

Bo-Htay

Palee

Apaijai

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…15 Other advantages are the easy application, low incidence of tumors, and the high survival rate of the animals throughout the experimental period. 16,17 It is important to note that, more recently, some dissonant reports concerning the effectiveness of this accelerated aging paradigm have been published. [18][19][20] Indeed, Parameshwaran and collaborators have concluded that the d-galactose treatment (intraperitoneal [i.p] injection of 100 mg/kg per day) in female C57BL/61 mice was not suitable for testing antioxidant compounds in mice.…”

Section: Introductionmentioning

confidence: 99%

“…17,24 We focused the present study on the hippocampal formation (HF), a brain limbic region particularly affected by aging [25][26][27][28][29] and presenting the hallmarks of neuronal agerelated deterioration. 14,16 Furthermore, because d-galactose may interfere with neurogenesis, 9,30 we decided to analyze the effects of the high-dose d-galactose on the neurogenesis of the hippocampal dentate granule cells using two neurogenic markers, doublecortin (DCX) and Ki-67, that are easily quantified in young rodents. 11,21 The focus on the neurogenic process assumes importance because new neurons can replace those lost through pathological or physiological events in the adult rodent brain, 31 but this capacity decreases with aging.…”

Section: Introductionmentioning

confidence: 99%

d-Galactose High-Dose Administration Failed to Induce Accelerated Aging Changes in Neurogenesis, Anxiety, and Spatial Memory on Young Male Wistar Rats

Cardoso

Magano

Marrana

et al. 2015

Rejuvenation Research

View full text Add to dashboard Cite

The model of accelerated senescence with the prolonged administration of d-galactose is used in anti-aging studies because it mimics several aging-associated alterations such as increase of oxidative stress and decline of cognition. However, there is no standardized protocol for this aging model, and recently some reports have questioned its effectiveness. To clarify this issue, we used a model of high-dose d-galactose on 1-month-old male Wistar rats and studied the hippocampus, one of the most affected brain regions. In one group (n = 10), d-galactose was daily administered intraperitoneally (300 mg/kg) during 8 weeks whereas age-matched controls (n = 10) were injected intraperitoneally with saline. A third group (n = 10) was treated with the same dose of d-galactose and with oral epigallocatechin-3-gallate (EGCG) (2 grams/L), a green tea catechin with anti-oxidant and neuroprotective properties. After treatments, animals were submitted to open-field, elevated plus-maze and Morris water maze tests, and neurogenesis in the dentate gyrus subgranular layer was quantified. There were no significant alterations when the three groups were compared in the number of doublecortin-and Ki-67-immunoreactive cells, and also on anxiety levels, spatial learning, and memory. Therefore, d-galactose was not effective in the induction of accelerated aging, and EGCG administered to d-galactose-treated animals did not improve behavior and had no effects on neurogenesis. We conclude that daily 300 mg/kg of d-galactose administered intraperitoneally may not be a suitable model for inducing age-related neurobehavioral alterations in young male Wistar rats. More studies are necessary to obtain a reliable and reproducible model of accelerated senescence in rodents using d-galactose.

show abstract

“…This sugar is commonly used in our daily life as table sugar. The root mechanism of aging process is still unclarified due to difficulties in performing experimental aging studies such as ethic problems, cost, time, and age‐related additional pathologies (Yanar et al, ). In order to solve these problems, researchers directed their researches on model organisms such as Caenorhabditis elegans , Mus musculus , Saccharomyces cerevisiae , Danio rerio, and D. melanogaster (Pandey & Nichols, ).…”

Section: Introductionmentioning

confidence: 99%

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

2018

View full text Add to dashboard Cite

We evaluated the effects of type of nutrients on redox status according to age and gender in Drosophila melanogaster. Flies were raised on different dose of sucrose and fixed concentration of protein containing media and separated according to their gender and age. Redox status biomarkers such as protein carbonyl groups, dityrosine, kynurenine, lipid hydroperoxides, advanced glycation end products, and Cu,Zn superoxide dismutase were assessed. We found that all of the males had higher levels of basal oxidative stress biomarkers except protein carbonyl. On the other hand, Cu,Zn‐superoxide dismutase activity was elevated. We found that high concentration of sucrose leads to impaired redox status. We also observed that high protein content in sucrose‐based media fecundity rate was increased and also transition to adult flies and survival ratio were decreased. Further studies are needed to focus on evaluation of the relation between median life span, nutritional mechanisms, and redox status biomarkers. Practical applications Aging is characterized by functional decline in whole body system. Unfortunately, many of the life span extension strategies may lead to age‐related disorders. It is widely accepted that impaired redox homeostasis is one of the signs of age‐related pathophysiological changes. Due to difficulties in the establishment and validation of mimetic aging models in rodents, D. melanogaster is becoming/getting widely used model organism in gerontological studies. Currently, high protein and low carbohydrate‐based nutrition regimes have become one of the most popular nutritional choice in aging population. Favorable nutrition type is considered one of the important factors for preventing age‐related pathologies and promote healthy aging. In this study, we aimed to evaluate redox modulatory effects of different nutrition types as combination of various sucrose and constant protein concentration on basal redox status in D. melanogaster. Due to evolutionally conservation, we hypothesize that similar findings may be observed in aging population. Favorable nutrition type may ameliorate or postpone age‐related degenerative processes and promote healthy aging.

show abstract

Protein and DNA Oxidation in Different Anatomic Regions of Rat Brain in a Mimetic Ageing Model

Cited by 67 publications

References 58 publications

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

Effects of d‐galactose‐induced ageing on the heart and its potential interventions

d-Galactose High-Dose Administration Failed to Induce Accelerated Aging Changes in Neurogenesis, Anxiety, and Spatial Memory on Young Male Wistar Rats

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

Contact Info

Product

Resources

About