Protein and DNA Polymorphisms among Three Colored Seeds of Phaseolus vulgaris L. Cultivars during Seven Subsequent Stages of their Development

Abstract

T HIS STUDY aimed to evaluate genetic variations among germplasms of three colored seeds of Phaseolus vulgaris cultivars (creamish yellow "strike", deep brown "contender" and reddish spotty pinto "wounder") during seven subsequent stages of seed development ranging from dormant seed to pollen grains. The evaluation based on the analysis of the data obtained from electrophoretic banding patterns of protein and DNA separated from each of the three studied cultivars, by using two different bioassays; sodium dodecyl sulfate polyacrylamide-gel electrophoresis (SDS-PAGE) and random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR). These analyses revealed major distinctive genetic variations among the three cultivars qualitatively and quantitatively. Besides, there were also variations in the percentages of polymorphisms in the produced protein and DNA bands due to the differences among their size, number, intensity, types, and gain or loss others. The highest number of polypeptide bands and amplified DNA products was scored at the contender cultivar, while the lowest number was scored at the strike cultivar during all stages of seed development. Meanwhile, the highest total number of polypeptide bands (64) and amplified DNA bands (108) were scored at the seedling and vegetative stages, respectively. This study concluded that data generated by the used bioassays were nearly equivalent, but not identical. Thus, it is better to be combined for reliable estimates of genetic variations among the three studied cultivars during their development to earn valuable genetic resources exploited for breeding new cultivars.