Protein and enzyme electrophoresis profiles of selected Candida species

Lehmann, Paul F.; Hsiao, Chiu-Bin; Salkin, Ira F.

doi:10.1128/jcm.27.3.400-404.1989

Cited by 31 publications

(30 citation statements)

References 15 publications

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“…Many papers has been published in last years on Candida classification using SDS-Page (Greenfield & Jones, 1981;Monod et al, 1990;Brondz & Olsen, 1991;Epstein et al, 1980;Lehmann et al, 1989;Shen et al, 1988). So, SDS-Page has been successfully used for classification and identification of many species of this group of microorganisms.…”

Section: Culture Mediamentioning

confidence: 99%

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

et al. 2001

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The application of gel electrophoresis and numerical analysis of yeast soluble proteins analysis to the investigation of 12 oral yeast strains belonging to five species is described. It involves one-dimensional electrophoresis of SDS-solubilized whole-cell proteins using different culture media for the cultivation of the cells, integration densitometries in the areas of the gels and percentages of the proteins extraction. These extracts were prepared from four isolates of Candida albicans, two of C. tropicalis, C. guilliermondii, C. parapsilosis and C. krusei. The extracts from whole-cells proteins using different culture media for the cultivation of the cells were fractionated by slab electrophoresis using a discontinuous buffer system. The corresponding patterns showed at least 36 polypeptides in the range of 14.4-200 kDa. Different isolates of each species were clearly different in each of the five species. The data obtained suggest that different nutritional compositions led to the expression of different proteins derived from alternatives metabolic pathways expressed by the electrophoretic profiles. The construction of a database of protein fingerprints and numerical analysis based on such data, may have some implications in the classification and identification of such species with epidemiological, ecological and taxonomic purposes. A well defined or synthetic culture media seems to be much properly.

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Section: Culture Mediamentioning

confidence: 99%

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

et al. 2001

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“…Electrophoretic variations in enzymes have been used only to a limited extent for differentiation of Candida spp. (3,17,18). Analysis of variations in eight enzymes among 37 isolates of C. albicans revealed 23 patterns (18).…”

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Epidemiological analysis of Candida albicans strains by multilocus enzyme electrophoresis

Caugant¹,

Sandven²

1993

J Clin Microbiol

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Genotypic diversity in a collection of 98 isolates of Candida albicans was assessed by multilocus enzyme electrophoresis. Four of the 10 enzyme loci studied were polymorphic. The electrophoretic patterns observed were compatible with those expected for a diploid organism. The 98 isolates were assigned to 14 electrophoretic types, each of which was represented by from 1 to 21 isolates. Samples from various clinical sites of seven bone marrow transplant patients treated in the same unit within a 13-month period were obtained repeatedly. Three patients were found to be colonized with more than one strain. In one patient, flucytosine-resistant strains were isolated after systemic antifungal treatment was started. These isolates had electrophoretic types different from those of the strains that colonized the patient before treatment. There was no evidence that cross-infection between these patients occurred in the hospital. The population structure of C. albicans is discussed in regard to the multilocus genotype data.

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“…Other methods used to identify yeasts include their presumptive identification upon primary isolation on differential media (7,20). Clinical strains of Catidida atbicans have been characterized using the germ-tube test (14), but a proportion of isolates were germ-tube negadve (19). A test for y5galactosaminidase activity has been Proposed as a more reliable substitute for the germ-tube test (18).…”

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Identification of oral yeasts by polyacrylamide gel electrophoresis

Maiden¹

1991

Oral Microbiology and Immunology

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Protein profiles of sonicated cells for 9 species of yeast isolated from oral samples were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and visualized with a silver stain. The profiles obtained on 12% acrylamide gels were distinct, and characteristic for 6 species of Candida, and Torulopsis glabrata, Yarrowia lipolytica, and Saccharomyces cerevisiae. Using this method, 79 fresh isolates of yeasts from saliva samples were identified; 58 as Candida albicans, 9 as Candida parapsilosis, 1 as Candida tropicalis, and 11 as T. glabrata. SDS-PAGE offers a rapid, convenient alternative or adjunct to yeast identification systems based on carbohydrate assimilation tests.

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Protein and enzyme electrophoresis profiles of selected Candida species

Cited by 31 publications

References 15 publications

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

Epidemiological analysis of Candida albicans strains by multilocus enzyme electrophoresis

Identification of oral yeasts by polyacrylamide gel electrophoresis

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