Protein‐ and peptide‐modified synthetic polymeric biomaterials

Krishna, Ohm D.; Kiick, Kristi L.

doi:10.1002/bip.21333

Cited by 186 publications

(147 citation statements)

References 156 publications

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“…Moreover, their rapid self-assembly enables cell encapsulation and irregular defect filling in both in vitro and in vivo applications. [11][12][13][14] In the present study, the histological and morphological observations at 4 weeks revealed significant changes in the number, diameter, and myelin sheath thickness of nerve fibers in the SF16 peptide hydrogel group after 4 weeks of implantation, indicating better nerve regeneration. These findings indicate that the SF16 peptide scaffold may have a positive effect in nerve regeneration, and therefore has potential as a matrix for nerve cell propagation.…”

Section: Histological Observationssupporting

confidence: 57%

“…Peptide hydrogel scaffolds not only have all of the advantages of traditional hydrogels but also do not use harmful materials to initiate the solution-gel transformation, therefore the degradation products are natural amino acids which can be metabolized. 10,13,14 Although many in vitro and in vivo experiments have demonstrated the advantages of using self-assembly peptide nanofibrous scaffolds in neural tissue engineering, lower mechanical strength and shorter degradation rates present as limitations in specific tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

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Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

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Background: Peripheral nerve injury is a common trauma, but presents a significant challenge to the clinic. Silk-based materials have recently become an important biomaterial for tissue engineering applications due to silk's biocompatibility and impressive mechanical and degradative properties. In the present study, a silk fibroin peptide (SF16) was designed and used as a component of the hydrogel scaffold for the repair of peripheral nerve injury. Methods: The SF16 peptide's structure was characterized using spectrophotometry and atomic force microscopy, and the SF16 hydrogel was analyzed using scanning electron microscopy. The effects of the SF16 hydrogel on the viability and growth of live cells was first assessed in vitro, on PC12 cells. The in vivo test model involved the repair of a nerve gap with tubular nerve guides, through which it was possible to identify if the SF16 hydrogel would have the potential to enhance nerve regeneration. In this model physiological saline was set as the negative control, and collagen as the positive control. Walking track analysis and electrophysiological methods were used to evaluate the functional recovery of the nerve at 4 and 8 weeks after surgery. Results: Analysis of the SF16 peptide's characteristics indicated that it consisted of a welldefined secondary structure and exhibited self-assembly. Results of scanning electron microscopy showed that the peptide based hydrogel may represent a porous scaffold that is viable for repair of peripheral nerve injury. Analysis of cell culture also supported that the hydrogel was an effective matrix to maintain the viability, morphology and proliferation of PC12 cells. Electrophysiology demonstrated that the use of the hydrogel scaffold (SF16 or collagen) resulted in a significant improvement in amplitude recovery in the in vivo model compared to physiological saline. Moreover, nerve cells in the SF16 hydrogel group displayed greater axon density, larger average axon diameter and thicker myelin compared to those of the group that received physiological saline. Conclusion: The SF16 hydrogel scaffold may promote excellent axonal regeneration and functional recovery after peripheral nerve injury, and the SF16 peptide may be a candidate for nerve tissue engineering applications.

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Section: Histological Observationssupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Wei¹,

Yao²,

Wang³

et al. 2013

IJN

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“…Moreover, bioactive peptides and functional groups, such as Arg-Gly-Asp (RGD), a cell adhesion peptide sequence, can be incorporated into synthetic hydrogels to mimic the extracellular matrix and direct cell-matrix interactions [65]. Thus, a promising future of synthetic hydrogels is the research of a system that can incorporate the features of natural hydrogels into synthetic hydrogels and possesses the advantages of each [66,67]. For example, RGD peptides were covalently grafted to poly(ethylene glycol) diacrylate (PEG-DA, MW 8000) and human foreskin fibroblasts could attach to and spread on the crosslinked RGD-PEGDA gel.…”

Section: Synthetic Hydrogelmentioning

confidence: 99%

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

2012

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Encapsulation of cells in hydrogel particles has been demonstrated as an effective approach to deliver therapeutic agents. The properties of hydrogel particles, such as the chemical composition, size, porosity, and number of cells per particle, affect cellular functions and consequently play important roles for the cell-based drug delivery. Microfluidics has shown unparalleled advantages for the synthesis of polymer particles and been utilized to produce hydrogel particles with a well-defined size, shape and morphology. Most importantly, during the encapsulation process, microfluidics can control the number of cells per particle and the overall encapsulation efficiency. Therefore, microfluidics is becoming the powerful approach for cell microencapsulation and construction of cell-based drug delivery systems. In this article, I summarize and discuss microfluidic approaches that have been developed recently for the synthesis of hydrogel particles and encapsulation of cells. I will start by classifying different types of hydrogel material, including natural biopolymers and synthetic polymers that are used for cell encapsulation, and then focus on the current status and challenges of microfluidic-based approaches. Finally, applications of cell-containing hydrogel particles for cell-based drug delivery, particularly for cancer therapy, are discussed.

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“…However, the advantages of using polymeric membranes to achieve, for example, higher stability or possible chemical functionalization represent a firm base on which to develop these new hybrid materials, as we will explain in our review. Here, we will not present the means to obtain protein-and peptide-polymer conjugates, as these have been reviewed recently [7].…”

Section: Introductionmentioning

confidence: 99%

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

et al. 2011

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Today, demand exists for new systems that can meet the challenges of identifying biological entities rapidly and specifically in diagnostics, developing stable and multifunctional membranes, and engineering devices at the nanometer scale. In this respect, bio-decorated membranes combine the specificity and efficacy of biological entities, such as peptides, proteins, and DNA, with stability and the opportunity to chemically tailor the properties of polymeric membranes. A smart strategy that serves to fulfill biological criteria is required, whereby polymer membranes come to mimic biological membranes and do not disturb but rather enhance the functioning and activity of a biological entity. Different approaches have been developed, exemplified by either planar or vesicular membranes, allowing insertion inside the polymer membrane or anchoring via functionalization of the membrane surface. Inspired by nature, but incorporating the strength provided by chemical design, bio-decorated polymer membranes represent a novel concept with great potential in diagnostics and therapeutics.

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Protein‐ and peptide‐modified synthetic polymeric biomaterials

Cited by 186 publications

References 156 publications

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Promotion of peripheral nerve regeneration of a peptide compound hydrogel scaffold

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

Bio-Decorated Polymer Membranes: A New Approach in Diagnostics and Therapeutics

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