2016
DOI: 10.1111/2041-210x.12680
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Protein and RNA extraction from mucosal swabs: a minimally invasive source of ecological data for studies of natural populations

Abstract: Summary Mucosal swabs have long been used to study various physiological processes in humans. In contrast, systematic sampling of mucosae is rare for wild animals, and except for its use as a source of DNA for population genetics, its potential as a tool to study physiologically relevant processes of natural populations has not been explored fully. We collected swabs from the rectal, genital, nasal and oral mucosae of California sea lion pups and investigated differences in RNA and protein yield, quality and… Show more

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Cited by 9 publications
(1 citation statement)
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References 76 publications
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“…One hundred and nine combined oral–cloacal swabs were assayed for pathogens. 24,39,46 Samples were collected between one and three times per individual. The only pathogen detected was terrapene herpesvirus 1, occurring in 3 of the 59 turtles (5.1%; 95% CI: 1.1–14.2%) sampled in June (n = 1), July (n = 1), and August (n = 1).…”
Section: Resultsmentioning
confidence: 99%
“…One hundred and nine combined oral–cloacal swabs were assayed for pathogens. 24,39,46 Samples were collected between one and three times per individual. The only pathogen detected was terrapene herpesvirus 1, occurring in 3 of the 59 turtles (5.1%; 95% CI: 1.1–14.2%) sampled in June (n = 1), July (n = 1), and August (n = 1).…”
Section: Resultsmentioning
confidence: 99%