Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

Shirai, Tsuyoshi; Furusawa, Haruhiko; Furukawa, Asuka; Ishige, Yuki; Uchida, Keiji; Miyazaki, Yasunari; Eishi, Yoshinobu; Inase, Naohiko

doi:10.1186/s12931-017-0555-4

Cited by 16 publications

(16 citation statements)

References 29 publications

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“…IGLL1, for example, has previously been reported in the uterus in a microarray experiment [27]. This protein was found in the protein component of the deeper layers of the shell [28] and appears to be a major secreted protein responsible for pigeon fanciers lung [29], but other than the strong indication that it is an epithelial secreted protein and its function remains obscure.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the uterus of hens laying eggs differing in cuticle deposition

et al. 2020

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Background: Avian eggs have a proteinaceous cuticle. The quantity of cuticle varies and the deposition of a good cuticle in the uterus (Shell-gland) prevents transmission of bacteria to the egg contents. Results: To understand cuticle deposition, uterus transcriptomes were compared between hens with i) naturally good and poor cuticle and, ii) where manipulation of the hypothalamo-pituitary-gonadal-oviduct axis produced eggs with or without cuticle. The highest expressed genes encoded eggshell matrix and cuticle proteins, e.g. MEPE (OC-116), BPIFB3 (OVX-36), RARRES1 (OVX-32), WAP (OVX-25), and genes for mitochondrial oxidative phosphorylation, active transport and energy metabolism. Expression of a number of these genes differed between hens laying eggs with or without cuticle. There was also a high expression of clock genes. PER2, CRY2, CRY1, CLOCK and BMAL1 were differentially expressed when cuticle deposition was prevented, and they also changed throughout the egg formation cycle. This suggests an endogenous clock in the uterus may be a component of cuticle deposition control. Cuticle proteins are glycosylated and glycosaminoglycan binding genes had a lower expression when cuticle proteins were deposited on the egg. The immediate early genes, JUN and FOS, were expressed less when the cuticle had not been deposited and changed over the egg formation cycle, suggesting they are important in oviposition and cuticle deposition. The uterus transcriptome of hens with good and poor cuticle deposition did not differ. Conclusions: We have gained insights into the factors that can affect the production of the cuticle especially clock genes and immediate early genes. We have demonstrated that these genes change their expression over the period of eggshell formation supporting their importance. The lack of differences in expression between the uterus of hens laying eggs with the best and worse cuticle suggest the genetic basis of the trait may lie outside the oviduct.

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Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the uterus of hens laying eggs differing in cuticle deposition

et al. 2020

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“…6-129.6). The prediction rule derived from the six factors had a sensitivity of 86% (95% CI, [79][80][81][82][83][84][85][86][87][88][89][90][91][92] and a specificity of 86% (95% CI, [81][82][83][84][85][86][87][88][89][90] with an HP prevalence of 45%. The receiveroperating characteristic (ROC) curve was 0.93 (95% CI, 0.90-0.95).…”

Section: Summary Of the Evidencementioning

confidence: 99%

“…Presently, such preparations are not yet commercially available or representative of all potential causative antigens of individual HP cases (eg, farmer's lung). 76,89,90 Since the performance characteristics and interpretation of the serum antigen-specific antibody test are often sitespecific and influenced by the prevalence of HP in the population being tested (ie, predictive value), the clinician ordering serum antigen-specific antibody testing should understand the validity and reliability of the test and the population sample type and size used to determine its diagnostic performance characteristics.…”

Section: Antigen-specific Antibody Testingmentioning

confidence: 99%

Diagnosis and Evaluation of Hypersensitivity Pneumonitis

Pérez

Travis

Lynch

et al. 2021

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BACKGROUND:The purpose of this analysis is to provide evidence-based and consensusderived guidance for clinicians to improve individual diagnostic decision-making for hypersensitivity pneumonitis (HP) and decrease diagnostic practice variability.STUDY DESIGN AND METHODS: Approved panelists developed key questions regarding the diagnosis of HP using the PICO (Population, Intervention, Comparator, Outcome) format. MEDLINE (via PubMed) and the Cochrane Library were systematically searched for relevant literature, which was supplemented by manual searches. References were screened for inclusion, and vetted evaluation tools were used to assess the quality of included studies, to extract data, and to grade the level of evidence supporting each recommendation or statement. The quality of the evidence was assessed using the GRADE (Grading of Recommendations, Assessment, Development, and Evaluation) approach. Graded recommendations and ungraded consensus-based statements were drafted and voted on using a modified Delphi technique to achieve consensus. A diagnostic algorithm is provided, using supporting data from the recommendations where possible, along with expert consensus to help physicians gauge the probability of HP. RESULTS:The systematic review of the literature based on 14 PICO questions resulted in 14 key action statements: 12 evidence-based, graded recommendations and 2 ungraded consensusbased statements. All evidence was of very low quality.INTERPRETATION: Diagnosis of HP should employ a patient-centered approach and include a multidisciplinary assessment that incorporates the environmental and occupational exposure history and CT pattern to establish diagnostic confidence prior to considering BAL and/or lung biopsy. Criteria are presented to facilitate diagnosis of HP. Additional research is needed on the performance characteristics and generalizability of exposure assessment tools and traditional and new diagnostic tests in modifying clinical decision-making for HP, particularly among those with a provisional diagnosis.

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“…The molecular weight of IgA in pigeons is 200‐220 kDa, and antigen‐antibody reactions were observed at 220 kDa 23,24 . Proteins at 50 kDa, 29‐32 kDa, 26 kDa, and 21 kDa in pigeon droppings are reportedly associated with disease onset 5,25‐27 . In this study, multiple bands were found in the serum of patients with bird‐related CHP at 37‐75 kDa.…”

Section: Discussionmentioning

confidence: 50%

“…23,24 Proteins at 50 kDa, 29-32 kDa, 26 kDa, and 21 kDa in pigeon droppings are reportedly associated with disease onset. 5,[25][26][27] In this study, multiple In this study, Western blotting was performed to investigate the presence or absence of antibodies to pigeon eggs. If there were no antibodies for pigeon eggs, the changes after the inhalation challenge test may be caused by inhalation stimuli rather than an allergic reaction.…”

Section: Discussionmentioning

confidence: 99%

Inhalation challenge test using pigeon eggs for chronic hypersensitivity pneumonitis

Okuda

Takemura

Mikami

et al. 2020

Clin Experimental Allergy

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Background: Chronic hypersensitivity pneumonitis (CHP) remains a diagnostic challenge. The process of collecting and extracting serum and droppings from causative animals for the inhalation challenge test is complicated and the risk of inducing disease progression exists. Objective: To investigate the utility and safety of an inhalation challenge test using pigeon eggs. Methods: Pigeon eggs were pasteurized and mixed with a saline solution to produce an inhalation fluid. An inhalation challenge test was conducted on 19 patients with bird-related CHP and 17 patients with interstitial lung disease other than bird-related CHP. To identify antigens in pigeon eggs, the antigen-antibody responses of the pigeon eggs and serum from patients were evaluated using Western blotting. Results: The mean changes in C-reactive protein, alveolar-arterial oxygen difference, erythrocyte sedimentation rate, and lactate dehydrogenase significantly increased by 0.32 mg/dL (P = .014), 7.8 Torr (P = .002), 1.4 mm/h (P = .012), and 5.4 U/mL (P = .0019), respectively, in bird-related CHP group compared to the control 24 hours after the inhalation challenge test. Furthermore, within 24 hours of the inhalation test, the mean forced vital capacity decreased by 2.3% in the bird-related CHP group compared with a decline of 0.05% in the control group (P = .035). Serum collected from seven bird-related CHP patients who underwent the inhalation challenge test and reacted to antigens with molecular weights of 37-75 KDa, and these molecular weights were consistent with egg albumin and globulin. Conclusion: Since a mild response was observed after the inhalation challenge test using pigeon eggs, this test was an obvious candidate for diagnosing bird-related CHP.

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Protein antigen of bird-related hypersensitivity pneumonitis in pigeon serum and dropping

Cited by 16 publications

References 29 publications

Transcriptome analysis of the uterus of hens laying eggs differing in cuticle deposition

Transcriptome analysis of the uterus of hens laying eggs differing in cuticle deposition

Diagnosis and Evaluation of Hypersensitivity Pneumonitis

Inhalation challenge test using pigeon eggs for chronic hypersensitivity pneumonitis

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