Protein-Based Nanoparticles as Drug Delivery Systems

Hong, Seyoung; Choi, Dong Wook; Kim, Hong Nam; Park, Chun Gwon; Lee, Wonhwa; Park, Hee Ho

doi:10.3390/pharmaceutics12070604

Cited by 358 publications

(227 citation statements)

References 152 publications

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“…Moreover, recently Ran HH et al [ 28 ] developed Colistin-loaded polydopamine nanospheres decorated with silver nanodots with improved antimicrobial and antibiofilm activity. In this contest, protein-based polymers such as albumin have been considered as drug carriers due to their non-toxicity, biocompatibility, biodegradability and poor immunogenic properties [ 29 ]. Albumin NPs have been largely proposed for the delivery of anticancer drugs and approved in the first-line treatment of metastatic breast cancer [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Antimicrobial and Antibiofilm Effect of New Colistin-Loaded Human Albumin Nanoparticles

et al. 2021

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Multidrug-resistant (MDR) Gram-negative bacteria (GNB), such as Acinetobacter and Klebsiella, are responsible for severe hospital-acquired infections. Colistin, despite its toxicity and low tissue penetration, is considered the last resort antibiotic against these microorganisms. Of concern, the use of Colistin has recently been compromised by the emergence of Colistin resistance. Herein, we developed a new formulation consisting of multifunctional chitosan-coated human albumin nanoparticles for the delivery of Colistin (Col/haNPs). Col/haNPs were in vitro characterized for encapsulation efficiency, drug release, stability and cytotoxicity and were evaluated for antibacterial activity against MDR GNB (Acinetobacter baumannii and Klebsiella pneumoniae). Col/haNPs showed sizes lower than 200 nm, high encapsulation efficiency (98.65%) and prolonged in vitro release of Colistin. The safety of the nanoformulation was demonstrated by a negligible cytotoxicity on human fibroblasts and hemolytic activity. Col/haNPs evidenced a high antibacterial effect with a significant decrease in MIC values compared to free Colistin, in particular against Col-resistant strains with a pronounced decline of bacterial growth over time. Moreover, Col/haNPs exhibited an inhibitory effect on biofilm formation that was 4 and 60 fold higher compared to free Colistin, respectively for Colistin susceptible and resistant A. baumannii. Our findings suggest that Col/haNPs could represent a promising Colistin nanocarrier with high antimicrobial activity on MDR GNB.

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Section: Discussionmentioning

confidence: 99%

Enhanced Antimicrobial and Antibiofilm Effect of New Colistin-Loaded Human Albumin Nanoparticles

et al. 2021

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“…Protein-based DDS have several advantages, such as biodegradability, stability, surface modification of particles, and ease of particle size control. Hong et al [ 131 ] have reviewed the general aspects concerning the preparation and characterization of different types of NPs-based on proteins—however, without a detailed discussion of the micellar DDS based on proteins. Therefore, in this paragraph, we review the literature data concerning the protein-based DDS from the last decade.…”

Section: Micellar Drug Delivery Systems Based On Proteinsmentioning

confidence: 99%

Micellar Drug Delivery Systems Based on Natural Biopolymers

Atanase

2021

Polymers

160

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The broad diversity of structures and the presence of numerous functional groups available for chemical modifications represent an enormous advantage for the development of safe, non-toxic, and cost-effective micellar drug delivery systems (DDS) based on natural biopolymers, such as polysaccharides, proteins, and peptides. Different drug-loading methods are used for the preparation of these micellar systems, but it appeared that dialysis is generally recommended, as it avoids the formation of large micellar aggregates. Moreover, the preparation method has an important influence on micellar size, morphology, and drug loading efficiency. The small size allows the passive accumulation of these micellar systems via the permeability and retention effect. Natural biopolymer-based micellar DDS are high-value biomaterials characterized by good compatibility, biodegradability, long blood circulation time, non-toxicity, non-immunogenicity, and high drug loading, and they are biodegraded to non-toxic products that are easily assimilated by the human body. Even if some recent studies reported better antitumoral effects for the micellar DDS based on polysaccharides than for commercial formulations, their clinical use is not yet generalized. This review is focused on the studies from the last decade concerning the preparation as well as the colloidal and biological characterization of micellar DDS based on natural biopolymers.

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“…For protein-based delivery systems, positively charged proteins provide the ability to condense nucleic acids, which enhances the efficiency of gene transfection [ 96 ]. Surface modification of peptide and protein-based systems with targeting moieties can improve the delivery of genome-editing components to specific sites [ 97 ]. Representative examples of peptide/protein-based delivery systems for genome-editing components are illustrated in Figure 5 .…”

Section: Nonviral Delivery Systems For Genome Editingmentioning

confidence: 99%

Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing

Kim

et al. 2020

Pharmaceutics

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Genome-editing technology has emerged as a potential tool for treating incurable diseases for which few therapeutic modalities are available. In particular, discovery of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system together with the design of single-guide RNAs (sgRNAs) has sparked medical applications of genome editing. Despite the great promise of the CRISPR/Cas system, its clinical application is limited, in large part, by the lack of adequate delivery technology. To overcome this limitation, researchers have investigated various systems, including viral and nonviral vectors, for delivery of CRISPR/Cas and sgRNA into cells. Among nonviral delivery systems that have been studied are nanovesicles based on lipids, polymers, peptides, and extracellular vesicles. These nanovesicles have been designed to increase the delivery of CRISPR/Cas and sgRNA through endosome escape or using various stimuli such as light, pH, and environmental features. This review covers the latest research trends in nonviral, nanovesicle-based delivery systems that are being applied to genome-editing technology and suggests directions for future progress.

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Protein-Based Nanoparticles as Drug Delivery Systems

Cited by 358 publications

References 152 publications

Enhanced Antimicrobial and Antibiofilm Effect of New Colistin-Loaded Human Albumin Nanoparticles

Enhanced Antimicrobial and Antibiofilm Effect of New Colistin-Loaded Human Albumin Nanoparticles

Micellar Drug Delivery Systems Based on Natural Biopolymers

Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing

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