Protein Binding of Corticosteroids in Undiluted Rat Plasma1

Keller, Nancy R.; Sendelbeck, LR; Richardson, U. Ingrid; Moore, Caylin Louis; Yates, F. Eugene

doi:10.1210/endo-79-5-884

Cited by 68 publications

(16 citation statements)

References 1 publication

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“…Ethinyloestradiol did not increase the corticosterone binding capacity of the plasma; this is in agreement with Gala & Westphal (1965). Keller, Sendelbeck, Richardson, Moore & Yates (1966), however, did observe a small increase in corticosteroid binding globulin, but only after 14 days of high doses of oestrogen given to male rats. These results are surprising in view of the many reports of an increase in corticosteroid binding activity after oestrogen treatment in man (Sandberg & Slaunwhite, 1959;Daughaday, Adler, Mariz, & Rasinski, 1962).…”

Section: Discussionsupporting

confidence: 84%

The actions of ethinyloestradiol on the pituitary‐adrenal system of the rat

1970

British J Pharmacology

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Summary1. Single doses of ethinyloestradiol stimulate the pituitary adrenal axis of the female rat during quiescent conditions. 2. After a stimulus which releases endogenous ACTH, there is an inhibition of the release of corticosterone from the adrenal gland, but an increase in the corticosterone stored in the gland.3. Ethinyloestradiol (1V0 mg/kg) injected acutely or daily for 7 days does not affect the corticosterone bindinlg capacity of plasma in female rats, or the clearance rate of an injection of corticosterone. 4. Prolonged treatment with ethinyloestradiol and ACTH inhibits the pituitary-adrenal response to a stress and to an ACTH injection, indicating a block at the adrenal and pituitary level. 5. The adrenal gland recovers more rapidly than the pituitary after cessation of the oestrogen treatment. 6. The inhibition of the pituitary-adrenal response to stress after oestrogen is probably caused by inhibition of cholesterol synthesis as suggested by others. Oestrogens can increase corticoid secretion during non-stress conditions, however, so precursor lack through exhaustion cannot be excluded. 7. It is suggested that the inhibition of the stress response after prolonged ACTH treatment is due to a decrease in sensitivity of the adrenal cortex after frequent ACTH stimulation, but the possibility that it is due to a reduction in circulating cholesterol cannot be ruled out.

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Section: Discussionsupporting

confidence: 84%

The actions of ethinyloestradiol on the pituitary‐adrenal system of the rat

1970

British J Pharmacology

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“…It is possible that the decrease in CBG induced by water restriction is a result of elevations in corticosterone that occur during the first 2 days of the water restriction protocol (unpublished observations), because it has been shown that corticosterone has a negative regulatory effect on CBG levels (13,21). These data do not support the hypothesis that acute changes in CBG account for the preferential clearance of corticosterone over aldosterone after drinking.…”

Section: Discussionmentioning

confidence: 92%

Differential regulation of adrenal corticosteroids after restriction-induced drinking in rats

Wotus

Engeland

2003

American Journal of Physiology-Regulatory, Integrative and Comp

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Wotus, Cheryl, and William C. Engeland. Differential regulation of adrenal corticosteroids after restriction-induced drinking in rats. Am J Physiol Regul Integr Comp Physiol 284: R183-R191, 2003. First published October 3, 2002 10.1152/ajpregu.00027.2002.-Water-restricted rats exhibit a rapid decrease in plasma corticosterone after drinking. The present study examined the effect of restriction-induced drinking on plasma aldosterone and plasma clearance of corticosterone. Rats were water restricted for 6-7 days and then killed before or 15 min after water administration; plasma and adrenal hormones were assayed. Plasma and adrenal corticosterone decreased after drinking without a change in plasma corticosteroid-binding globulin; plasma ACTH decreased or did not change. In contrast, plasma aldosterone did not change or increased after drinking; plasma renin activity was elevated by water restriction and increased further after drinking. In another experiment, rats were adrenalectomized, and corticosterone and aldosterone were replaced with pellets and osmotic minipumps, respectively. Rats were water restricted and killed. There was a small decrease in plasma corticosterone but no change in aldosterone after drinking in adrenalectomized animals. These data suggest that changes in plasma steroids after restriction-induced drinking result from zone-specific responses of the adrenal to known secretagogues, with minimal contribution from increased plasma clearance.corticosterone; aldosterone; corticosteroid-binding globulin; steroid clearance; dehydration THE RAT ADRENAL CORTEX is divided into two morphologically and functionally distinguishable steroidogenic zones. The inner zona fasciculata/reticularis produces corticosterone in response to its primary secretagogue, ACTH, which is released from the anterior pituitary. The outer zona glomerulosa produces the mineralocorticoid aldosterone in response to multiple factors, including ANG II, an end product of the renin-angiotensin system. Increased secretion of corticosterone and aldosterone represents a homeostatic response to stressful stimuli. In contrast to the response following stress, a marked reduction in plasma and adrenal corticosterone is observed immediately after water is presented to rats restricted to a limited amount of water each day (8,18,24,31,40). Although evidence suggests that this rapid decline in corticosterone may occur though mechanisms acting at the level of the adrenal cortex (8, 40), it is not known whether restriction-induced drinking affects aldosterone in a similar manner. Previous studies have shown increases in plasma aldosterone after deprivation-induced drinking in dogs (37) and sheep (3), whereas there were no changes in plasma aldosterone after drinking in dehydrated humans (15). However, rapid changes in corticosterone and aldosterone after drinking have not been investigated concurrently in the same animal model of dehydration. Moreover, there are inconsistencies concerning the effect of dehydration alone on aldosterone secretion ...

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“…Thus, deficits in ovarian output resulting from a delayed onset of puberty could account for the present effects. Estrogen may act to stim ulate corticosterone through increasingplasma levelsofcorticosteroid-bindingglobulin(CBG) [10], and thus a decreased corticosterone re sponse also could reflect changes at the liver site of CBG production. However, CBG lev els do not differ between steroid-treated and control males and females at 25 days of age [Erskine et a/., unpubl.…”

Section: Discussionmentioning

confidence: 99%

Effects of Neonatal Hydrocortisone Treatment on Pituitary and Adrenocortical Response to Stress in Young Rats

Erskine¹,

Geller²,

Yuwiler³

1979

Neuroendocrinology

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Administration of corticosteroids to rat pups within the first several days of life results in retardation of several behavioral, neurophysiological, and biochemical developmental patterns. At 25 days of age, animals treated neonatally with 1.0, 0.5, or 0.1 mg hydrocortisone acetate showed dose-dependent decreases in plasma ACTH following 2.5 min continuous exposure to ether. On day 20, plasma corticosterone values did not differ between these groups 15 min after ether stress, but lower values were seen 60 min after ether in animals treated at birth with 0.5 mg hydrocortisone. At 45-48 days of age, hydrocortisone-treated animals exposed to one of 2 different stressors showed decreased plasma corticosterone response to stress; females had lower corticosterone levels following both stressors, while males showed suppressed corticosterone levels following exposure to the mild (novelty) but not to the intense (ether) stress. These data demonstrate that neonatal exposure to hydrocortisone results in decreased CNS-pituitary responsivity to stress at 20–25 days of age, and that the adrenocortical response to stress is impaired at 45–48 days of age.

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Protein Binding of Corticosteroids in Undiluted Rat Plasma1

Cited by 68 publications

References 1 publication

The actions of ethinyloestradiol on the pituitary‐adrenal system of the rat

The actions of ethinyloestradiol on the pituitary‐adrenal system of the rat

Differential regulation of adrenal corticosteroids after restriction-induced drinking in rats

Effects of Neonatal Hydrocortisone Treatment on Pituitary and Adrenocortical Response to Stress in Young Rats

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