Protein bioengineering

Jungbauer, Alois

doi:10.1002/biot.200690007

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…Other promising potential clinical uses of exosomes include exosome‐based cancer vaccines tested in different clinical trials and exosome‐based vaccines for emerging infectious diseases such as Ebola and influenza virus among others. [ 185,186 ] Good manufacturing practice (GMP) guidelines place limitations on the manufacture and handling of exosomes for use in clinical trials. In most of these clinical trials, the source of exosomes was mesenchymal stem cells (MSCs), dendritic cells (DCs) or plant derived exosomes.…”

Section: Perspectives On Clinical Applications Of Exosomesmentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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Exosomes, a subgroup of extracellular vesicles, are important mediators of long‐distance intercellular communication and are involved in a diverse range of biological processes such as the transport of lipids, proteins, and nucleic acids. Researchers, seeing the problems caused by the toxic effects and clearance of synthetic nanoparticles, consider exosomes as an interesting alternative to such nanoparticles in the specific and controlled transport of drugs. In recent years, there have been remarkable advances in the use of exosomes in cancer therapeutics or for treating neurological diseases, among other applications. The objective of this work is to analyze studies focused on exosomes used in drug delivery system, present and future applications in this field of research are discussed based on the results obtained.

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Section: Perspectives On Clinical Applications Of Exosomesmentioning

confidence: 99%

Advances in Exosomes‐Based Drug Delivery Systems

Gutiérrez-Millán

Díaz

Lanao

et al. 2020

Macromolecular Bioscience

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“…[16] Catalytic biofilms present one interesting possibility to realize continuous reactionf ormats.B iofilms are sessile microbial, matrix-enclosed communities,a ttaching to different materials at the interfacet oa na queous phase. [17,18] Due to theiri ntrinsic properties such as natural immobilization, physical robustness, resistance against toxic chemicals and long-term stability they have been evaluated as an alternative to planktonically growing whole cell biocatalysts,e nabling continuous processesu nder biologically challenging conditions.…”

Section: Introductionmentioning

confidence: 99%

Multistep Synthesis of (S)‐3‐Hydroxyisobutyric Acid from Glucose usingPseudomonas taiwanensisVLB120 B83 T7 Catalytic Biofilms

2015

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Thec hiral building block (S)-3-hydroxyisobutyric acid [(S)-3-HIBA] was produced either by conversion of isobutyric acid or directly from glucose utilizing cells of Pseudomonas taiwanensis VLB120 B83 T7 as catalysts.T his strain carriesapoint mutation in the gene encoding 3-hydroxyisobutyrate dehydrogenase (mmsB), leading to its inactivation.T he maximal specific activity in resting-cell biotransformations using isobutyric acid as substrate was 4.9 AE 0.4 Ug cdw À1 .O verexpression of the 2-ketoisovalerate pathwayg enes alsS,i lvC, and ilvD,a nd the introduction of kivd encoding 2-ketoacid decarboxylase resulted in the efficient fermentatives ynthesiso f( S )-3-HIBAd irectly from glucose.U pt o2 2mM( 2.3 g L À1 ) (S)-3-HIBAwere produceda t3 .7 AE 0.3 Ug cdw À1 in repeated batch experiments without observable product degradation. Utilizing ab iofilm reactor it was possible to continuously produce up to.O verall, the conversion of isobutyric acid to (S)-3-HIBAw as found to be the rate-limiting step,l eading to the accumulation of am ixture of (S)-3-hydroxyisobutyric acid andi sobutyric acid. This study demonstratesf or the first time the production of (S)-3-HIBAf rom renewable carbon in shake flasks and biofilm reactors and sets the stage for furthero ptimizations towards the efficient production of 3-HIBAa nd its derivatives in continuous fermentations.

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Protein bioengineering

Cited by 2 publications

References 29 publications

Advances in Exosomes‐Based Drug Delivery Systems

Advances in Exosomes‐Based Drug Delivery Systems

Multistep Synthesis of (S)‐3‐Hydroxyisobutyric Acid from Glucose usingPseudomonas taiwanensisVLB120 B83 T7 Catalytic Biofilms

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