Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

Harayama, Hiroshi; Minami, Kenta; Kishida, Kazumi; Noda, Taichi

doi:10.1002/rmb2.12021

Cited by 29 publications

(19 citation statements)

References 110 publications

(162 reference statements)

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“…Several studies have shown that the characteristics of sperm quality, such as motility, capacitation, and morphology, affect conception in cattle [4, 29,30,31,32]. However, to our knowledge, there are few studies showing that only improving bovine sperm quality could lead to beneficial effects on fertilization or embryo development.…”

Section: Discussionmentioning

confidence: 99%

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Umezu

Yajima²,

Hiradate

et al. 2019

Journal of Reproduction and Development

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Previously, we reported that neurotensin (NT), which is expressed in the uterus and oviduct, enhanced bovine sperm capacitation and acrosome reactions. As NT mRNA expression in bovine oviducts increases dramatically in the follicular phase, we hypothesized that NT modulates fertilization and subsequent conception in cattle. The objective of this study was to evaluate the effect of NT on embryo development and blastocyst quality. The rate of embryo cleavage was significantly increased by the addition of NT to the fertilization medium. Furthermore, the total number of cells and numbers of cells in the inner cell mass of blastocysts were significantly increased by NT during in vitro fertilization (IVF). These results suggested that NT enhanced the efficiency of early bovine embryo development and blastocyst quality. The expression of NT receptors (NTRs) in sperm, testes, oocytes, and cumulus cells was evaluated to determine whether NT acted via NTRs in sperm alone or in both male and female reproductive cells during IVF. Immunocytochemistry and reverse transcription polymerase chain reaction revealed that NTR1 and NTR2 were expressed in sperm and testes, but not in oocytes and cumulus cells. We propose that NT selectively acts upon sperm via NTR1 and NTR2 during IVF to improve the cleavage rate and quality of blastocysts, which are important determinants of sperm quality for successful conception. This research supports our hypothesis that NT acts as a key modulator of fertilization and conception in cattle. Further studies are necessary to apply our findings to the industrial framework of bovine reproduction.

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Section: Discussionmentioning

confidence: 99%

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Umezu

Yajima²,

Hiradate

et al. 2019

Journal of Reproduction and Development

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“…1,16,23,24 Thus, it is necessary to make detailed researches on the interspecies differences in the sperm functions in order to elucidate mechanisms for the expression of fertilizing ability in mammalian spermatozoa. According to our previous investigations of bull and boar spermatozoa, [25][26][27][28][29] and PKA are localized in the specific subcellular segments including the connecting and principal pieces, and proteins are preferentially phosphorylated in these segments of the spermatozoa treated with the cAMP analog to induce capacitation-related events leading to hyperactivation. These results are consistent with our hypothesis that connecting pieces of bull and boar spermatozoa have unique cAMP-dependent controllers for regulation of the capacitation-related events leading to hyperactivation.…”

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confidence: 99%

Flagellar hyperactivation of bull and boar spermatozoa

Harayama

2018

Reprod Medicine & Biology

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BackgroundIn mammals, flagellar hyperactivation is indispensable to sperm fertilization with oocytes in vivo, although there are species differences in regulatory mechanisms for this event. In this study, I reviewed researches regarding hyperactivation of bull and boar spermatozoa, in comparison with those of spermatozoa from other species.MethodsRecent publications regarding sperm hyperactivation were collected and summarized.Results (Main findings)In bull and boar spermatozoa, there are two types of hyperactivation “full‐type hyperactivation and nonfull‐type hyperactivation” which are equivalent to anti‐hock hyperactivation and pro‐hock hyperactivation of mouse spermatozoa, respectively, on the basis of the flagellar parts exhibiting asymmetrical beating. Full‐type hyperactivation is initiated in response to a rapid increase of cytoplasmic Ca2+ in the connecting/middle and principal pieces by the mobilization of this divalent ion from extracellular space and internal store through cation channels. Regulatory molecules for the increase of cytoplasmic Ca2+ in the connecting/middle pieces are probably different from those in the principal pieces.ConclusionI have proposed a hypothesis on the regulation of full‐type hyperactivation by the distinct signaling cascades leading to the increase in cytoplasmic Ca2+ between the connecting/middle and principal pieces of bull and boar spermatozoa.

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“…Furthermore, in the examination for the selection of lot numbers of straws of frozen spermatozoa, some of the testing straws are thawed and subjected to the evaluation of sperm motility. Thereafter, the remaining straws with the same lot numbers as the testing straws that have passed the examination of sperm motility are used for AI [ 1 , 2 ]. Motility of the spermatozoa is one of the most important characteristics associated with their fertilizing ability.…”

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confidence: 99%

Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation

Yamada

Sakase

Fukushima

et al. 2018

Journal of Reproduction and Development

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Progressive movement of spermatozoa has conventionally been regarded as a good indicator of motility. However, bull spermatozoa exhibit two types of progressive movement: progressive/planar movement without rotation and progressive/helical movement with rotation. The aim of this study was to reconsider the evaluation criteria of bull ejaculated sperm motility in the context of rotation. Here, we compared the movement patterns of ejaculated spermatozoa with relatively high and low protein kinase A (PKA)-mediated signaling activities, because sperm motility is positively regulated by PKA-mediated signaling activities. We prepared sperm samples with high and low PKA-mediated signaling activities by suspending spermatozoa in media containing either the stimulator (NaHCO3) or inhibitor (KH-7) of adenylyl cyclase 10, and we then investigated movement patterns and relative velocities using a microscopic high-speed camera and recording system. In the control medium without NaHCO3 and KH-7, most spermatozoa exhibited round/planar movement without rotation and asymmetrical bends in the principal pieces. NaHCO3 significantly promoted changes in movement patterns from round/planar movement to progressive/planar movement (without rotation) as well as symmetrization of flagellar bends and increased relative velocities. KH-7 significantly increased spermatozoa exhibiting progressive/helical movement (with rotation), decreased relative velocities, and symmetrized flagellar bends with a reduction in their size. These indicate that progressive/planar movement (without rotation) and fast movement characterize the movement patterns of bull ejaculated spermatozoa with high PKA-mediated signaling activities. A sign of reduced PKA-mediated signaling activity is not only slow movement but also helical movement (with rotation). Thus, it is beneficial to add a new parameter of “rotation” to the evaluation criteria of bull ejaculated sperm motility.

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Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

Cited by 29 publications

References 110 publications

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Improvement in blastocyst quality by neurotensin signaling via its receptors in bovine spermatozoa during <i>in vitro</i> fertilization

Flagellar hyperactivation of bull and boar spermatozoa

Reconsideration of the evaluation criteria for bull ejaculated sperm motility in the context of rotation

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