Protein Bodies of the Soybean

Abstract

Suimmary. Some

Immunochemical Study of Changes in Reserve Proteins of Germinating Soybean Seeds

“…The 11S component (e) acquires higher electrophoretic mobility during the first 3 days of germination. After thi,s period, its mobility is relatively tinchanged, and iut is found to be presenit even up to 16 than that of the other componenfts. The increased mdbility acquired by the 11S and 7S components duiring germination can be explained by deamidation of the asparagine and glultamine residues of these proteins producing an increase in negative charges by expoisture of carboxyil groups.…”

“…The protein bodies were isolated from the soybean meal by density gradient centriftugation at 24,000 X g as described by Tombs (16) 4B) which may be an indication of 2 different polymeric forms. Component f near the cathodic end of the column is also antigenLiic and produces the coirrespondinig immunoprecipitin arc f. In conclusion, the proteins presenit in the protein bodies consist of at least 6 immunochemically different components 2 of which, the 11S and 7S components, have been isolated and found to be homogeneous by several criteria (3,4,5,8).…”

“…However, serious discrepancies exiist as to the nature of the proteins present in these inclusions. Tomabs (16) repor-ted that only 1 protein could be detected in them, namely, glycinin, the major soybean protein, which he found identical with the 11S component as isolated in crude form by Wolf and Briggs (17). Saio and Watanabe (14) concluded that the protein bodies consist of complex protein components which are similar to those of the whole water extract of soybeans.…”

“…Usually the reserve proteins are stored in granular cytoplasmic inclusions called protein bodies or aleurone grains. Morphological changes of protein bodies in soybeans during development (2) and germination (16) have been observed by electron microscopy. The protein bodies of the soybean have been isolated by 2 different methods (14,16).…”

“…Morphological changes of protein bodies in soybeans during development (2) and germination (16) have been observed by electron microscopy. The protein bodies of the soybean have been isolated by 2 different methods (14,16). However, serious discrepancies exiist as to the nature of the proteins present in these inclusions.…”