Protein Carbonyl and the Methionine Sulfoxide Reductase System

Moskovitz, Jackob; Oien, Derek B.

doi:10.1089/ars.2009.2809

Cited by 54 publications

(46 citation statements)

References 88 publications

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“…This capacity could be related to MSRs' ability to function as ROS scavengers in concert with some of their protein targets, which would not be affected by sulfoxidation (54,55). Such a general role is supported by the negative relationship between the level of MSR activity and the levels of proteins oxidized by carbonylation in numerous organisms (56). Thus, the involvement of MSRs in ROS scavenging could occur in seeds to limit the extent of damage resulting from the oxidative conditions reigning during maturation and imbibition.…”

Section: Discussionmentioning

confidence: 99%

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

102

103

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Seeds are in a natural oxidative context leading to protein oxidation. Although inevitable for proper progression from maturation to germination, protein oxidation at high levels is detrimental and associated with seed aging. Oxidation of methionine to methionine sulfoxide is a common form of damage observed during aging in all organisms. This damage is reversible through the action of methionine sulfoxide reductases (MSRs), which play key roles in lifespan control in yeast and animal cells. To investigate the relationship between MSR capacity and longevity in plant seeds, we first used two Medicago truncatula genotypes with contrasting seed quality. After characterizing the MSR family in this species, we analyzed gene expression and enzymatic activity in immature and mature seeds exhibiting distinct quality levels. We found a very strong correlation between the initial MSR capacities in different lots of mature seeds of the two genotypes and the time to a drop in viability to 50% after controlled deterioration. We then analyzed seed longevity in Arabidopsis thaliana lines, in which MSR gene expression has been genetically altered, and observed a positive correlation between MSR capacity and longevity in these seeds as well. Based on our data, we propose that the MSR repair system plays a decisive role in the establishment and preservation of longevity in plant seeds.seed deterioration | seed viability | redox systems | antioxidant enzymes S uccessful crop implantation is crucial for maximal plant yield and relies on seed physiological quality, which is acquired at the end of development, during maturation. Quality is expressed by the capacity of seeds to germinate in a fast and homogenous manner before and after storage (seed longevity) and to provide proper seedling establishment in various environments (seed vigor). Quality is controlled by genetic traits and varies greatly among species and cultivars. The establishment of quality also depends on the prevailing environmental conditions during seed development and storage (1, 2).During the maturation phase, mechanisms are put in place to prevent and repair desiccation damage, as well as to allow survival of seeds in the dry state (3-5). Nonetheless, drying of seeds on mother plants can occur prematurely in drought conditions, leading to low-quality seeds (6-8). Thus, in the context of a warming climate, producing good-quality seeds represents a global challenge to sustain current yield levels (9, 10).The mechanisms underlying seed quality are controlled in part by abscisic acid (ABA), the phytohormone involved in plant responses to dehydration that is produced during seed maturation. Of note, ABA triggers the accumulation of late-embryogenesis abundant proteins, heat-shock proteins (HSPs), and storage proteins (11). Late-embryogenesis abundant proteins and HSPs are thought to primarily protect cell structures when water is removed. Reserve proteins, through their sensitivity to reactive oxygen species (ROS), play a determinant role in limiting oxidative damage...

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Section: Discussionmentioning

confidence: 99%

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Châtelain

Satour

Laugier

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

102

103

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“…Ageing processes are related to the oxidation of methionine residues in CaM (Bigelow and Squier, 2005), and oxidized CaM is less efficient than native CaM at activating AC1 (Lübker et al, 2015a,b). Thus, one could envisage that reduction of oxidized methionine residues in CaM by increased expression of methionine sulfoxide reductase is therapeutically useful in CNS diseases associated with impaired learning and memory (Moskovitz and Oien, 2010;Lübker et al, 2015a,b).…”

Section: +mentioning

confidence: 99%

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases

Dessauer¹,

Watts²,

Ostrom³

et al. 2017

Pharmacol Rev

173

198

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“…The oxidation of Met alters its hydrophobicity, which can lead to conformational changes that affect proteinprotein interactions or enzymatic activity (266). Two distinct stereoisomers are formed around the sulfur-S-and R-epimers, and these are reversed by methionine sulfoxide reductase A (MsrA) and B (MsrB), respectively (99,(195)(196)(197). MsrA can also act independently of redox signaling as an S-epimer oxidase (164).…”

Section: Methioninementioning

confidence: 99%

“…The reducing equivalents needed for this reaction are provided by the Trx system. Protein carbonyl adducts had been used as a marker for methionine sulfoxide modifications, as the current assumption is that the structural changes associated with sulfoxidation increase the likelihood of further modification and promote protein carbonyl formation (195 (34). Another technique that has been used is collision-induced dissociation (CID) and tandem MS, which along with gas-phase fragmentation can determine the sequence and position of methionine sulfoxide.…”

Section: Methioninementioning

confidence: 99%

“…One significant difference between the two in-forming protein carbonyls is that histidine, similar to Arg and proline, forms aldehydes, while the Lys oxidation product, adipic semialdehyde leads to direct carbonylation. The reader is referred to several recent excellent reviews for further information on this topic (18,195,302). Carbonylation has been detected by antibodies, enrichment-labeling techniques, and mass spectrometry (271).…”

Section: His and Lysmentioning

confidence: 99%

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Cardiovascular Redox and Ox Stress Proteomics

Kumar

Calamaras

Haeussler

et al. 2012

Antioxidants & Redox Signaling

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Significance: Oxidative post-translational modifications (OPTMs) have been demonstrated as contributing to cardiovascular physiology and pathophysiology. These modifications have been identified using antibodies as well as advanced proteomic methods, and the functional importance of each is beginning to be understood using transgenic and gene deletion animal models. Given that OPTMs are involved in cardiovascular pathology, the use of these modifications as biomarkers and predictors of disease has significant therapeutic potential. Adequate understanding of the chemistry of the OPTMs is necessary to determine what may occur in vivo and which modifications would best serve as biomarkers. Recent Advances: By using mass spectrometry, advanced labeling techniques, and antibody identification, OPTMs have become accessible to a larger proportion of the scientific community. Advancements in instrumentation, database search algorithms, and processing speed have allowed MS to fully expand on the proteome of OPTMs. In addition, the role of enzymatically reversible OPTMs has been further clarified in preclinical models. Critical Issues: The identification of OPTMs suffers from limitations in analytic detection based on the methodology, instrumentation, sample complexity, and bioinformatics. Currently, each type of OPTM requires a specific strategy for identification, and generalized approaches result in an incomplete assessment. Future Directions: Novel types of highly sensitive MS instrumentation that allow for improved separation and detection of modified proteins and peptides have been crucial in the discovery of OPTMs and biomarkers. To further advance the identification of relevant OPTMs in advanced search algorithms, standardized methods for sample processing and depository of MS data will be required. Antioxid. Redox Signal. 17, 1528-1559.

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Protein Carbonyl and the Methionine Sulfoxide Reductase System

Cited by 54 publications

References 88 publications

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases

Cardiovascular Redox and Ox Stress Proteomics

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