Protein carbonylation associated with nickel liberation in orthodontic gingival overgrowth

Páez, Jennifer Orozco; Méndez-Rodríguez, María A.; Rodríguez-Cavallo, Erika; Díaz-Caballero, Antonio; Méndez-Cuadro, Darío

doi:10.1016/j.archoralbio.2021.105103

Cited by 8 publications

(3 citation statements)

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“…reported that azithromycin had a significant effect on the fibroblastic part of pathological gingival enlargement, and that azithromycin increased reduced matrix-metalloproteinase (MMP)-1 activities (20) .…”

Section: Results Of Clinical Examinationmentioning

confidence: 99%

Impact of Azithromycin on Gingival Enlargement

mustafs¹,

saffar²,

mahmood³

2022

RDENTJ

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Aims: This study was aimed to evaluate the effect of azithromycin in reducing pathological gingival enlargement. Materials and methods: twelve adult New Zealand male rabbits weighing (900gm-1250mg) and almost the same in age and circumstance were chosen in this study. All animals with pathological gingival enlargement were clinically examined by using a periodontal probe and measuring their gingival sulcus depth. Then, six of them were slaughtered and considered the control group and the other six were experimental group, and at the end of study a specimens of oral mucosa were taken for immunohistochemical examinations. Results: Clinical examination shows there is no reduction in gingival enlargement in the control group while this enlargement decreased in the experimental group after use of azithromycin. Immunohistochemical analysis shows mild matrix metalloproteinase-1(MMP1) expression in the control group and moderate to strong MMP1 expression in sub-epithelial of gingiva in the experimental group. Conclusion: This study establishes that azithromycin is clinically and immunohistochemically effective in the reduction of pathological gingival enlargement.

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Section: Results Of Clinical Examinationmentioning

confidence: 99%

Impact of Azithromycin on Gingival Enlargement

mustafs¹,

saffar²,

mahmood³

2022

RDENTJ

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“…In this study we explored OS and genotoxicity before and after starting xed orthodontic appliance, and clinical evaluation of patients showed gingival overgrowth which is a condition that commonly develops during orthodontic treatment, because oral hygiene can be easily altered due to additive and irritative effects of orthodontics appliances [19]. However, a recent study demonstrated that the gum of patients with gingival overgrowth by orthodontic appliances contains higher nickel residues and carbonylation of its proteins [20]. In this study overgrowth was detected, suggesting that it could be a signal of oxidative stress associated with metal release in orthodontic treatment.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress and genotoxicity in oral epithelial cells of subjects undergoing Fixed Orthodontic Appliances

Angeles-Estrada

Pérez-Soto

Pérez-Vielma

et al. 2023

Preprint

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Objectives The aim of this study was to analyze changes in oxidative stress (OS) and the genotoxic damage in oral epithelial cells caused by fixed orthodontic appliances. Materials and methods Samples of oral epithelial cells were obtained from fifty-one healthy voluntary subjects who had an indication for orthodontic treatment. The samples were obtained before and after 6- and 9-months starting treatment. OS was evaluated by quantitation of 8-hydroxy-2’deoxyguanosine (8-OHdG) and by relative gene expression of antioxidant enzymes Superoxide Dismutase (SOD) and Catalase (CAT). DNA degradation and instability were evaluated by multiplex PCR and fragment analysis for human identification. Results Quantitation of 8-OHdG evidenced increase during treatment, although not statistically significant. SOD showed increase of 2.5 and 2.6-fold after 6 and 9 moths of treatment. CAT shows an increase of 3-fold, after six months of treatment, while after 9 months of treatment the level expression decreased to level similar to that before treatment. DNA degradation was found in 8% and 12% of DNA samples after 6 and 9 months of treatment,respectively, while DNA instability was detected in only 2% and 8 % of DNA samples after 6 and 9 months of treatment, respectively. Conclusions Results show light changes in OS and genotoxicity after fixed orthodontic appliances, but also suggesting a biological adaptation response to treatment after 6 months. Clinical relevance OS in buccal cavity represents risk to oral and systemic diseases. Implementation of antioxidant supplementation, use of thermoplastic materials, and time reduction during orthodontic therapy may reduce the risk.

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“…Recent studies have shown that gingival overgrowth can also be induced by orthodontic therapy [13]. Gingival overgrowth due to orthodontics has several possible causes: the accumulation of bacterial plaque and tartar caused by improper oral hygiene [13,14]; the material from which the orthodontic appliance is made [15]; overloading the periodontium by exercising excessive forces [16].…”

Section: Discussionmentioning

confidence: 99%