Protein complexes in bacterial and yeast mitochondrial membranes differ in their sensitivity towards dissociation by SDS

Gubbens, Jacob; Slijper, Monique; Kruijff, Ben de; Kroon, Anton I.P.M. de

doi:10.1016/j.bbapap.2008.08.020

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…Yeast has been previously used to demonstrate the effect of SDS on biological membranes, showing that micelles of SDS may penetrate the membrane through pores in the yeast cell wall and destroy the membrane [10]. In defense against SDS surplus, yeast cells increase the expression levels of genes involved in oxidative stress which might be caused by its effect on membrane structure, carbon metabolism, or DNA repair [2].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Cao

et al. 2020

BMC Microbiol

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Background: Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl sulfate surfactants. Toxicological information on SDS is accumulating, however, mechanisms of SDS toxicity regulation remain poorly understood. In this study, the relationship between the SDS-sensitive mutants and their intracellular ROS levels has been investigated. Results: Through a genome-scale screen, we have identified 108 yeast single-gene deletion mutants that are sensitive to 0.03% SDS. These genes were predominantly related to the cellular processes of metabolism, cell cycle and DNA processing, cellular transport, transport facilities and transport routes, transcription and the protein with binding function or cofactor requirement (structural or catalytic). Measurement of the intracellular ROS (reactive oxygen species) levels of these SDS-sensitive mutants showed that about 79% of SDS-sensitive mutants accumulated significantly higher intracellular ROS levels than the wild-type cells under SDS stress. Moreover, SDS could generate oxidative damage and up-regulate several antioxidant defenses genes, and some of the SDSsensitive genes were involved in this process. Conclusion: This study provides insight on yeast genes involved in SDS tolerance and the elevated intracellular ROS caused by SDS stress, which is a potential way to understand the detoxification mechanisms of SDS by yeast cells.

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Section: Introductionmentioning

confidence: 99%

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Cao

et al. 2020

BMC Microbiol

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“…MIC was estimated using broth dilution according to a slightly modified EUCAST protocol [ 24 ]. Reference and clinical strains were sub-cultured on SDA and were grown in laboratory at 37 °C in Sabouraud Dextrose Broth (SDB).…”

Section: Methodsmentioning

confidence: 99%

Increased Absorption and Inhibitory Activity against Candida spp. of Imidazole Derivatives in Synergistic Association with a Surface Active Agent

Aonofriesei

2023

Microorganisms

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This paper’s purpose was to evaluate the interaction between three imidazole derivatives, (2-methyl-1H-imidazol-1-yl)methanol (SAM3), 1,1′-methanediylbis(1H-benzimidazole (AM5) and (1H-benzo[d]imidazol-1-yl)methanol 1-hydroxymethylbenzimidazole (SAM5) on the one hand, and sodium dodecyl sulphate (SDS) on the other, as antifungal combinations against Candida spp. Inhibitory activity was assessed using the agar diffusion method and Minimal Inhibitory Concentration (MIC) and showed moderate inhibitory activity of single imidazole derivatives against Candida spp. The mean value of MIC ranged from 200 µg/mL (SAM3) to 312.5 µg/mL (SAM3), while for SDS the MIC was around 1000 µg/mL. When used in combination with SDS, the imidazole derivatives demonstrated an improvement in their antifungal activity. Their MIC decreased over five times for AM5 and over seven times for SAM3 and SAM5, respectively, and ranged from 26.56 µg/mL (SAM3) to 53.90 µg/mL (AM5). Most combinations displayed an additive effect while a clear synergistic effect was recorded in only a few cases. Thus, the FIC Index (FICI) with values between 0.311 and 0.375 showed a synergistic effect against Candida spp. when SDS was associated with SAM3 (three strains), SAM5 (two strains) and AM5 (one strain). The association of imidazole derivatives with SDS led to the increased release of cellular material as well as the intracellular influx of crystal violet (CV), which indicated an alteration of the membrane permeability of Candida spp. cells. This favored the synergistic effect via increasing the intracellular influx of imidazoles.

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“…The first class of lipids for which photoreactive bioorthogonal (bifunctional) probes were developed were phosphatidylcholines (PCs). 25,27 Towards synthesizing these PC probes, both of the lipid tails of this molecule were appended with a terminal azide, and the choline moiety of the head group was conjugated with benzophenone or arylazide (Fig. 3A).…”

Section: Bifunctional Lipid Probesmentioning

confidence: 99%

Photoreactive bioorthogonal lipid probes and their applications in mammalian biology

2023

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Protein complexes in bacterial and yeast mitochondrial membranes differ in their sensitivity towards dissociation by SDS

Cited by 6 publications

References 40 publications

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae

Increased Absorption and Inhibitory Activity against Candida spp. of Imidazole Derivatives in Synergistic Association with a Surface Active Agent

Photoreactive bioorthogonal lipid probes and their applications in mammalian biology

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