Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs

Bartlett, Jennifer; Albertolle, Matthew E.; Wohlford‐Lenane, Christine L.; Pezzulo, Alejandro A.; Zabner, Joseph; Niles, Richard K.; Fisher, Susan J.; McCray, Paul B.; Williams, Katherine E.

doi:10.1152/ajplung.00056.2013

Cited by 34 publications

(31 citation statements)

References 87 publications

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“…Similarly, lyophilizing BALF samples without desalting results in high concentrations of sodium chloride, which can denature proteins. The most commonly utilized BALF concentration technique is the centrifugal filter (Amicon Ultra‐15 or Centricon‐10) to a 10‐fold concentration of immunoglobulin, but, even this technique results in a 20% loss of specific IgE and IgG …”

Section: Discussionmentioning

confidence: 99%

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

White

Moore-Colyer

Marti

et al. 2019

Veterinary Internal Medicne

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Background Severe asthma in horses, known as severe equine asthma (SEA), is a prevalent, performance‐limiting disease associated with increased allergen‐specific immunoglobulin E (IgE) against a range of environmental aeroallergens. Objective To develop a protein microarray platform to profile IgE against a range of proven and novel environmental proteins in SEA‐affected horses. Animals Six SEA‐affected and 6 clinically healthy Warmblood performance horses. Methods Developed a protein microarray (n = 384) using protein extracts and purified proteins from a large number of families including pollen, bacteria, fungi, and arthropods associated with the horses, environment. Conditions were optimized and assessed for printing, incubation, immunolabeling, biological fluid source, concentration techniques, reproducibility, and specificity. Results This method identified a number of novel allergens, while also identifying an association between SEA and pollen sensitization. Immunolabeling methods confirmed the accuracy of a commercially available mouse anti‐horse IgE 3H10 source (R2 = 0.91). Biological fluid source evaluation indicated that sera and bronchoalveolar lavage fluid (BALF) yielded the same specific IgE profile (average R2 = 0.75). Amicon centrifugal filters were found to be the most efficient technique for concentrating BALF for IgE analysis at 40‐fold. Overnight incubation maintained the same sensitization profile while increasing sensitivity. Reproducibility was demonstrated (R2 = 0.97), as was specificity using protein inhibition assays. Arthropods, fungi, and pollens showed the greatest discrimination for SEA. Conclusions and Clinical Importance We have established that protein microarrays can be used for large‐scale IgE mapping of allergens associated with the environment of horses. This technology provides a sound platform for specific diagnosis, management, and treatment of SEA.

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Section: Discussionmentioning

confidence: 99%

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

White

Moore-Colyer

Marti

et al. 2019

Veterinary Internal Medicne

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“…Additionally, airway mucins are thought to contribute to antiviral defenses by interacting with viruses in ways that impede their movement and/or prevent their uptake (48)(49)(50). Proteomic analysis indicates that many of the antimicrobial factors found in human airways can also be detected in porcine ASL, such as lactoferrin, lysozyme, SP-A, SP-D, SLPI, and numerous mucins including MUC1, -2, -4, -5B, -5AC, -13, - 16, and -19 (51). While LL-37 is unique to humans, porcine ASL contains a related molecule, the cathelicidin protegrin-1 (51), which displays antiviral activity against several different viruses (52,53).…”

Section: Discussionmentioning

confidence: 99%

Airway Surface Liquid Has Innate Antiviral Activity That Is Reduced in Cystic Fibrosis

Berkebile¹,

Bartlett²,

Alaiwa³

et al. 2020

Am J Respir Cell Mol Biol

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While chronic bacterial infections and inflammation are associated with progressive lung disease in cystic fibrosis (CF) patients, much less is known regarding the contributions of respiratory viral infections to this process. Clinical studies suggest that antiviral host defenses may be compromised in individuals with CF, and CF airway epithelia exhibit impaired antiviral responses in vitro. Here, we used the CF pig model to test the hypothesis that the antiviral activity of respiratory secretions is reduced in CF. We developed an in vitro assay to measure the innate antiviral activity present in airway surface liquid (ASL) from CF and non-CF pigs. We found that tracheal and nasal ASL from newborn non-CF pigs exhibited dose-dependent inhibitory activity against several enveloped and encapsidated viruses including Sendai virus (SeV), respiratory syncytial virus (RSV), influenza A, and adenovirus. Importantly, we found that the anti-SeV activity of nasal ASL from newborn CF pigs was significantly diminished relative to non-CF littermate controls. This diminution of extracellular antiviral defenses appears to be driven, at least in part, by the differences in pH between CF and non-CF ASL. These data highlight the novel antiviral properties of native airway secretions, and suggest the possibility that defects in extracellular antiviral defenses contribute to CF pathogenesis.

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“…One of the key inducers of inflammatory responses is NFK-B (26). Activation of NFK-B results from interactions between polyaromatic hydrocarbons found in DENPs with the intracellular Aryl-hydrocarbon receptors [29,30]. Also, increase of nuclear factor kappa B by diesel exhaust particles in mouse epidermal cells through phosphatidylinositol 3-kinase/Akt signaling pathway [29].…”

Section: Discussionmentioning

confidence: 99%

Effects of Mesenchymal Stem Cells and Their Derived Microvesicles on Pulmonary Toxicity Induced by Petrol Exhaust Nanoparticle; Histological and Immuno-Histochemical Study

Salam¹,

Faruk²,

Fouad³

et al. 2019

ARRB

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Background: Diesel vehicles exhaust contains toxic nanoparticles that drastically affect lung tissue due to their direct cytotoxic effects, induction of oxidative stress, inflammatory signaling pathways and DNA damage. Mesenchymal stem cells (MSCs) exhibit anti-inflammatory effects and efficient regenerative capacity in chronic lung diseases. Objectives: Evaluation of the effects of MSCs and MSCs-derived micro vesicles (MSCs-MVs) on pulmonary toxicity induced by diesel exhaust nanoparticles (DENPs). Materials and Methods: Sixty male rats were equally divided into: Group I (Control rats), Group II (DENPs group) received repeated doses of DENPs (180μg/rat) intratracheally every other day for 6 days, Group III (MSCs group) received MSCs intravenously (3×106 cells) after the last dose of DENPs and Group IV (MSCs-MVs group) received MSCs-MVs (0.5 mg/mL) intravenously after the last dose of DENPs. Lung tissue were subjected to histological and immunohistochemical assessment. Inflammatory cytokines and bronchoalveolar lavage fluid (BALF) contents of inflammatory cells, albumin, LDH and total proteins were evaluated. Results: Histological picture of lung tissue in DENPs group showed numerous collapsed alveoli, thick interalveolar septa and marked cellular infiltration. Elastic fibers were markedly decreased by DENPs. Increased optical density of NF-κB/p65 immunoreactivity. Bronchoalveolar lavage fluid showed significant elevation of inflammatory cytokines (TNF-a, IL-6), polymorphonuclear leukocytes (PMN), neutrophils, macrophages, LDH, total proteins and albumin. Treatment with either MSCs or MSCs-MVs led to a significant amelioration of all of the aforementioned studied parameters. Conclusion: MSCs-MVs and MSCs showed significant therapeutic effects against DENPs damaging effects on the lung tissues via their regenerative capacity and anti-inflammatory effects.

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Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs

Cited by 34 publications

References 87 publications

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

Development of a comprehensive protein microarray for immunoglobulin E profiling in horses with severe asthma

Airway Surface Liquid Has Innate Antiviral Activity That Is Reduced in Cystic Fibrosis

Effects of Mesenchymal Stem Cells and Their Derived Microvesicles on Pulmonary Toxicity Induced by Petrol Exhaust Nanoparticle; Histological and Immuno-Histochemical Study

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