Protein composition of coronavirus OC 43

SUMMARYHaemagglutinating encephalomyelitis virus (HEV), a member of the coronavirus group, was adapted for growth in adult pig thyroid cell cultures and purified by ammonium sulphate precipitation and rate zonal centrifugation through sucrose gradients. Polyacrylamide gel electrophoresis of samples of purified virus revealed the presence of five polypeptides, four of which contained carbohydrate. The molecular weights of these proteins were I8OOOO (gp I8O), I25 ooo (gp t25), IOOOOO (gp IOO), 56o00 (p 56) and 26500 (gp 26"5). After treatment of the virus with the non-ionic detergent Nonidet P4o, two subviral components were isolated. As RNA-containing particle, sedimenting in sucrose gradients at the same rate as untreated virus, was analysed and found to contain two polypeptides, p 56 and gp 26"5. The second complex sedimented at a much slower rate and contained three glycoproteins gp I8O, gp t25 and gp too. Comparison of these findings with data published for other members of the coronavirus group is discussed.

Section: Discussionmentioning

confidence: 93%

The Polypeptides of Haemagglutinating Encephalomyelitis Virus and Isolated Subviral particles

Pocock

Garwes

1977

“…On the other hand, it is known that the membrane-associated glycopolypeptides of TGEV and the human coronaviruses OC 43 and z29 E are not measurably digested by bromelain (Hierholzer et al 1972;Garwes & Pocock, I975;Hierholzer, t976). This suggests that the arrangement of glycoprotein in the envelope of HEV is similar to that of MHV, but different from that in the other species mentioned.…”

Section: Isolation Of Two Surface Glycoproteinsmentioning

confidence: 99%

Characterization and Isolation of Structural Polypeptides in Haemagglutinating Encephalomyelitis Virus

Callebaut

Pensaert

1980

SUMMARYHaemagglutinating encephalomyelitis virus (HEV), a member of the coronavirus family, was purified and analysed by SDS-polyacrylamide gel electrophoresis. It was shown to contain eight polypeptides, seven of which were glycosylated. They had apparent tool. wt. of 180000 (GP T8o), I3OOOO (GP T3o), I2oooo (GP tzo) 760oo (GP 76), 64ooo (VP 64), 54ooo (GP 54), 32000 (GP 32) and 3I ooo (GP 31). Electrophoresis of virus samples dissociated under varying conditions showed that GP 54 and GP I2O could be interpreted as larger products of GP 3I and GP 32 and of GP 76, respectively. GP 76 also appeared as a dimer with a mol. wt. of 14o00o (GP t4o) in the absence of fl-mercaptoethanol. Subviral particles, obtained by treatment with bromelain, banded at a slightly lower density than the intact virus and lacked surface projections. Analysis of these particles indicated that GP I8o, GP I3o and GP 76 are associated with the virus projections. A small part of GP 31 and GP 32 also appeared to protrude from the lipid envelope, since 2o % of each molecule was sensitive to digestion. Two glycoproteins, GP i 30 and GP 76, were solubilized with the detergent Triton X-Ioo and separated by rate zonal centrifugation. According to its activity in indirect haemagglutination tests, GP 76 was considered to be a monovalent haemagglutinin subunit.

“…The polypeptide composition of several mammalian coronaviruses has been determined, namely human coronavirus (HCV) strains 229E (Hierholzer, 1976: Macnaughton, 1980) and OC43 (Hierholzer et al, 1972), transmissible gastroenteritis virus (TGEV) of pigs (Garwes & Pocock, 1975;Garwes et al, 1976), haemagglutinating encephalomyelitis virus (HEV) of pigs (Pocock & Garwes, 1977), mouse hepatitis virus (MHV) strains A59 (Sturman, 1977;Bond et al, 1979), JHM (Wege et al, 1979: Bond et al, 1979 and MHV3 (Macnaughton, 1980). In general, the polypeptides of these coronaviruses can be assigned to four size-classes (Sturman, 1977).…”

Section: Introductionmentioning

confidence: 99%

Structural Polypeptides of Coronavirus IBV

Cavanagh¹

1981

SUMMARYAvian infectious bronchitis virus (IBV) was grown and radiolabelled with 35S-methionine, 3H-leucine and 3H-glucosamine in de-embryonated chicken eggs. Approximately 12 different polypeptides were clearly detected by SDSpolyacrylamide gel electrophoresis of virus preparations. Growth of IBV in chorioallantoic membrane cells labelled with 35S-methionine indicated that most of these polypeptides, and additional ones, some of which were gtycosylated, were host components. Five polypeptides appeared to be virus-coded, with apparent tool. wt. of 94 x 103, 84 x 10 3, 54 x 10 3, 30 × 103 and 28 × 103. Four of these, p94, p84, p30 and p28, were glycosylated. The virion spikes appeared to be composed of p94 and p84, while p30 and p28 were partially embedded in the virion membrane. By analogy with other reports, p54 is the nucleocapsid polypeptide.