Protein content and pattern during mucus gland 
maturation and its ecdysteroid control
in honey bee drones

Colonello-Frattini, Nínive Aguiar; Hartfelder, Klaus

doi:10.1051/apido:2003019

Cited by 36 publications

(52 citation statements)

References 43 publications

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“…Since mating has an effect on vitellogenesis, or on the maturation of oocytes in the ovary of insects (Chen 1984, Coleman et al 1995, it is possible that these glands may produce substances that regulate fertility and the sexual behavior of the females (Dallacqua & Cruz-Landim 2003). According to Colonello & Hartfelder (2003), the protein content increases in males of A. mellifera from newly emerged immature drones to sixday old drone. After this period, the number of protein bands in electrophoretic gels reduce, when at eight days old drones In solitary species, such as Anthidium maculosum L., the males are territorial and the females mate multiple times (Alcock et al 1977).…”

Section: Resultsmentioning

confidence: 99%

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Ferreira¹,

Abdalla²,

Kerr³

et al. 2004

Neotrop. Entomol.

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Anatomia Comparada dos Órgãos do Aparelho Reprodutor Masculino de 51 Espécies de AbelhasRESUMO -A anatomia dos órgãos internos do aparelho reprodutor de machos (ARM) adultos e pupas foi comparada em 51 espécies de abelhas, incluindo representantes de seis famílias. Foram obtidos quatro tipos diferentes de ARM. O tipo I está presente em machos das famílias mais basais (Colletidae, Andrenidae e Halictidae) e é caracterizado por três túbulos seminíferos por testículo, o qual é quase totalmente envolvido pela membrana escrotal. O tipo II é um tipo intermediário entre os tipos I e III e está presente em Mellitidae e Megachilidae, como também em alguns Apidae estudados, sendo caracterizado por possuir dutos deferentes pós-vesiculares fora da membrana escrotal e possuir três ou quatro túbulos seminíferos por testículo, exceto Apis mellifera L., a qual possui secundariamente um número aumentado de túbulos. O tipo III foi achado somente nos Apidae estudados e é caracterizado por apresentar os testículos e dutos genitais (exceto o duto deferente pós-vesicular) encapsulados separadamente, as glândulas acessórias são bem desenvolvidas e o duto ejaculador é calibroso, apresentando fissuras em sua parede externa, as quais podem ocorrer também no tipo II. O tipo IV está presente exclusivamente na tribo Meliponini e é caracterizado pela ausência de glândulas acessórias. PALAVRAS-CHAVE: Glândula acessória, genitália, morfologiaABSTRACT -The anatomy of the internal organs of the male reproductive apparatus (MRA) of adults and pupae was compared among 51 species of bees, including representatives of six families. Four different types of MRA were found. The type I is present in males of the less derived families (Colletidae, Andrenidae, and Halictidae) and is characterized by three seminiferous tubules per testis, which are almost completely enveloped by the scrotal membrane. The type II is an intermediary between types III and I and is present in Melittidae and Megachilidae, as well as in some Apidae studied, being characterized by post-vesicular deferent ducts outside the scrotal membrane and by three or four seminiferous tubules per testis, except for Apis mellifera L., which has a secondarily increased number of tubules. Type III was only found in the Apidae studied and is characterized by separately encapsulated testes and genital ducts (except for the post-vesicular deferent duct). Accessory glands are well developed, and the ejaculatory duct is thickened with fissures in its wall, which may also occur in the type II. Type IV is present exclusively in the tribe Meliponini, and is characterized by the absence of accessory glands.

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Section: Resultsmentioning

confidence: 99%

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Ferreira¹,

Abdalla²,

Kerr³

et al. 2004

Neotrop. Entomol.

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show abstract

“…The juvenile titer gradually increases (Giray and Robinson, 1996) due to an enhanced hormone synthesis by the corpora allata (Tozetto et al, 1995). Concomitantly, the ecdysteroid titer decreases markedly within the first day after emergence before it goes through a small peak around day 8, and then, for the rest of the drone's life cycle, remains at basal levels (Colonello and Hartfelder, 2003). Based on these findings, we addressed the question as to whether and how these key hormones in insect development and reproduction may affect the maturation program of the mucus glands.…”

Section: Multiple Mating and Mucus Gland Proteins In Honey Beesmentioning

confidence: 98%

“…Not surprisingly, therefore, hormones, especially JH and ecdysteroids, have been shown to play a role in sexual maturation and accessory gland activity in a number of insect species (Happ, 1992;Ismail and Gillot, 1995;Gillot, 1996), including the honey bee For the honey bee, we could recently show that especially ecdysteroids are potent modulators of protein synthesis in the mucus glands during sexual maturation (Colonello and Hartfelder, 2003).…”

Section: Male Accessory Gland (Mag) Products and Their Function In Inmentioning

confidence: 99%

“…First, we performed a qualitative and quantitative analysis on proteins secreted by the glandular epithelium into the lumen of the mucus glands (Colonello and Hartfelder, 2003). The protein content in this secretion increases almost fifteen fold during the first five days of the adult life cycle of a honey bee drone.…”

Section: Multiple Mating and Mucus Gland Proteins In Honey Beesmentioning

confidence: 99%

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She’s my girl - male accessory gland products and their function in the reproductive biology of social bees

Colonello-Frattini

Hartfelder

2005

Apidologie

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-Male accessory gland products have become a major issue in insect reproduction. They are a means of transport for sperm and can form a mating plug, and also have specific compounds that can modify the behavior and physiology of mated females. We briefly review the structure and function of accessory gland products in insects, especially in the fruitfly and some orthopterans and lepidopterans, and draw parallels to what is currently known in social bees. The structure of the mating sign differs considerably in this group. In bumble bees it consists of a viscous mass of a dipeptide and fatty acids, with linoleic acid affecting female behavior. The honey bees show considerable species-specific variation in their mating signs. In the cavity-dwelling species it is the mucus gland which provides the mass of the mating sign, and this glands undergoes a hormonally controlled sexual maturation in its program of protein synthesis. The stingless bees lack mucus glands altogether, and their mating sign consists of the ruptured genital capsule of the male. We discuss the structure of the mating sign and of its components in relation to the mating and reproductive biology of these groups of highly eusocial bees.honeybee / bumblebee / Meliponini / reproductive tract / mating sign / Apis / Bombus

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“…In several insects, mating is the primary factor that triggers oocyte ripening in the ovary, indicating the presence of factors in seminal fluid that trigger oogenesis (Ando et al 1996;Coffelt and Vick 1987;Fan et al 1999;Foster 1993;Giebultowicz et al 1991;Rafaeli and Gileadi 1995;Raina et al 1994;Shorey et al 1968;Webster and Carde 1984). Nonetheless, when analyzing mucus gland secretions, which are introduced with semen into the genital tract of honey bee queens, Colonello and Hartfelder (2003) did not find evidence that drone accessory gland proteins are necessary for ovarian development in A. mellifera .…”

Section: Discussionmentioning

confidence: 95%

Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

2015

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-Newly emerged virgin queens are frequently imprisoned in cages either inside or outside of colonies before delivery to a new hive. Ovary integrity and proper functioning is the primary factor in a queen's success as the colony mother. In this work, histological studies on ovaries are used to evaluate the effect of virgin queen imprisonment both out and inside the colony. The results show that the ovarian follicles of virgin queens maintained out of the colony advance only until the beginning of differentiation of oocytes and nurse cells, the vitellarium does not differentiate, and cell death is observed. For virgin queens caged inside a colony, oogenesis progresses until nurse cell and oocyte differentiation is completed, and the vitellarium shows initial differentiation. The results suggest that the best method for a head start of a queen's fertility is to maintain her inside the colony until introduction into a new hive.apiculture / caged queen / cell death / egg production / histology

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Protein content and pattern during mucus gland maturation and its ecdysteroid control in honey bee drones

Cited by 36 publications

References 43 publications

Comparative anatomy of the male reproductive internal organs of 51 species of bees

Comparative anatomy of the male reproductive internal organs of 51 species of bees

She’s my girl - male accessory gland products and their function in the reproductive biology of social bees

Beekeeping practice: effects of Apis mellifera virgin queen management on ovary development

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