Protein corona: Opportunities and challenges

Zanganeh, Saeid; Spitler, Ryan; Erfanzadeh, Mohsen; Alkilany, Alaaldin M.; Mahmoudi, Morteza

doi:10.1016/j.biocel.2016.01.005

Cited by 154 publications

(98 citation statements)

References 46 publications

(59 reference statements)

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“…Protein structural modification and approachability of adsorbed proteins should be carefully considered in the tailoring of preformed protein NPs for effective therapeutic activities mediated through binding with relevant cell receptors. 114 Protein directionality decides the flexibility and availability of optimal binding sites and protein function. Therefore, the directionality of the protein as well as the degree of conjugation/adsorption is essential for NP construction and design in drug delivery.…”

Section: Future Perspectivesmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

534

343

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After administration of nanoparticle (NP) into biological fluids, an NP–protein complex is formed, which represents the “true identity” of NP in our body. Hence, protein–NP interaction should be carefully investigated to predict and control the fate of NPs or drug-loaded NPs, including systemic circulation, biodistribution, and bioavailability. In this review, we mainly focus on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect. Validated correlative models for NP biological responses mainly based on protein corona fingerprints of NPs are more highly accurate than the models solely set up from NP properties. Based on these models, effectiveness as well as the toxicity of NPs can be predicted without in vivo tests, while novel cell receptors could be identified from prominent proteins which play important key roles in the models. The ungoverned protein adsorption onto NPs may have generally negative effects such as rapid clearance from the bloodstream, hindrance of targeting capacity, and induction of toxicity. In contrast, controlling protein adsorption by modifying NPs with diverse functional proteins or tailoring appropriate NPs which favor selective endogenous peptides and proteins will bring promising therapeutic benefits in drug delivery and targeted cancer treatment.

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Section: Future Perspectivesmentioning

confidence: 99%

Protein corona: a new approach for nanomedicine design

Nguyen¹,

Lee²

2017

IJN

534

343

View full text Add to dashboard Cite

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“…The protein corona formation depends on several physicochemical properties of NPs such as size, shape, surface charge, or hydrophobicity and can influence cellular uptake in vitro. [16][17][18][19][20][21][22][23] Therefore, the protein corona can also influence NP functionality and their biological effects. 24,25 For example, transferrin coating was shown to enhance the endocytosis of lipid-coated NPs in A549 cells in vitro and furthermore to have the best inhibitory effect on tumor growth in vivo compared to the other used NPs.…”

mentioning

confidence: 99%

The influence of surface charge on serum protein interaction and cellular uptake: studies with dendritic polyglycerols and dendritic polyglycerol-coated gold nanoparticles

Bewersdorff

Vonnemann

Kanik

et al. 2017

IJN

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Nanoparticles (NPs) have gained huge interest in the medical field, in particular for drug delivery purposes. However, binding of proteins often leads to fast NP uptake and rapid clearance, thereby hampering medical applications. Thus, it is essential to determine and control the bio–nano interface. This study investigated the serum protein interactions of dendritic polyglycerols (dPGs), which are promising drug delivery candidates by means of two dimensional gel electrophoresis (2DE) in combination with mass spectrometry. In order to investigate the influence of surface charge, sulfated (sulfated dendritic polyglycerol [dPGS]) and non-sulfated (dPGOH) surfaces were applied, which were synthesized on a gold core allowing for easier separation from unbound biomolecules through centrifugation. Furthermore, two different sizes for dPGS were included. Although size had only a minor influence, considerable differences were detected in protein affinity for dPGS versus dPGOH surfaces, with dPGOH binding much less proteins. Cellular uptake into human CD14 + monocytes was analyzed by flow cytometry, and dPGOH was taken up to a much lower extent compared to dPGS. By using a pull-down approach, possible cellular interaction partners of serum pre-incubated dPGS-Au20 NPs from the membrane fraction of THP-1 cells could be identified such as for instance the transferrin receptor or an integrin. Clathrin-mediated endocytosis was further investigated using chlorpromazine as an inhibitor, which resulted in a 50% decrease of the cellular uptake of dPGS. This study could confirm the influence of surface charge on protein interactions and cellular uptake of dPGS. Furthermore, the approach allowed for the identification of possible uptake receptors and insights into the uptake mechanism.

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“…However, there is no conclusive evidence for the mechanism of SiO 2 NPs inhibition of activated sludge total respiration although indirect evidence from fatty acid methyl ester (FAME) analysis of activated sludge suggests that SiO 2 NPs altered the composition of the microbial membrane lipids [24]. The potential biological effects of SiO 2 NPs on activated sludge warrants further investigations, especially because in nanotoxicity the key role of the interaction of nanoparticles (including SiO 2 NPs) with proteins, which are also a component of activated sludge extracellular polymeric substances (EPS), has been recognized recently [28][29][30].…”

Section: Potential Biological Effect Of Sio 2 Nps On Activated Sludgementioning

confidence: 99%

Retention of Silica Nanoparticles in a Lab-Scale Membrane Bioreactor: Implications for Process Performance and Membrane Fouling

Sibag

Lee

Kim³

et al. 2016

Water

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Abstract:In conventional activated sludge (CAS) involving aerobic biological processes, the retention of silica nanoparticles (SiO 2 NPs) has no detrimental effect on chemical oxygen demand (COD) and ammonia nitrogen (NH 3 -N) removal. However, for the membrane bioreactor (MBR) system, which is also based on the activated sludge process in addition to the membrane separation process, it has implications not only on the process performance but also on membrane fouling. To investigate these two implications in lab-scale experiments, we continuously operated a control MBR and two experimental MBRs, in which the 28 nm SiO 2 NPs and 144 nm SiO 2 NPs were added separately to the influent at a final concentration of 100 mg/L. Although the retention of SiO 2 NPs in the MBR, as confirmed by dynamic light scattering (DLS) analysis, did not compromise the COD and NH 3 -N removal, it resulted in substantial increases in the transmembrane pressure (TMP) suggesting the onset of membrane fouling. Analyses by batch-dead end filtration revealed the same fouling trend as observed during the continuous MBR experiments; membrane fouling is aggravated in the presence of SiO 2 NPs. This was evident from permeate flux decline of between 30% and 74% at very low TMP (5 kPa) and the further increases in the total resistance.

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Protein corona: Opportunities and challenges

Cited by 154 publications

References 46 publications

Protein corona: a new approach for nanomedicine design

Protein corona: a new approach for nanomedicine design

The influence of surface charge on serum protein interaction and cellular uptake: studies with dendritic polyglycerols and dendritic polyglycerol-coated gold nanoparticles

Retention of Silica Nanoparticles in a Lab-Scale Membrane Bioreactor: Implications for Process Performance and Membrane Fouling

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